MCM4 (NM_182746) Human Untagged Clone

CAT#: SC107549

MCM4 (untagged)-Human minichromosome maintenance complex component 4 (MCM4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MCM4
• Synonyms: CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD; P1-CDC21
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_182746, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCGTCCCCGGCGTCGACCCCGAGCCGCCGCGGCAGCCGGCGTGGAAGGGCCACCCCCGCCCAGACGC
  CTCGGAGTGAGGATGCCAGGTCATCTCCCTCTCAGAGACGTAGAGGCGAGGATTCCACCTCCACGGGGGA
  GTTGCAGCCGATGCCAACCTCGCCTGGAGTGGACCTGCAGAGCCCTGCTGCGCAGGACGTGCTGTTTTCC
  AGCCCTCCCCAAATGCATTCTTCAGCTATCCCTCTTGACTTTGATGTTAGTTCACCACTGACATACGGCA
  CTCCCAGCTCTCGGGTAGAGGGAACCCCAAGAAGTGGTGTTAGGGGCACACCTGTGAGACAGAGGCCTGA
  CCTGGGCTCTGCACAGAAGGGCCTGCAAGTGGATCTGCAGTCTGACGGGGCAGCAGCAGAAGATATAGTG
  GCAAGTGAGCAGTCTCTAGGCCAAAAACTTGTGATCTGGGGAACAGATGTAAATGTGGCAGCATGCAAAG
  AAAACTTTCAGAGATTTCTTCAGCGTTTTATTGACCCTCTGGCTAAAGAAGAAGAAAATGTTGGCATAGA
  TATTACTGAACCTCTATACATGCAACGACTTGGGGAGATTAATGTTATTGGTGAGCCATTTTTAAATGTG
  AACTGTGAACACATCAAATCATTTGACAAAAATTTGTACAGACAACTCATCTCTTACCCACAGGAAGTTA
  TTCCAACTTTTGACATGGCTGTCAATGAAATCTTCTTTGACCGTTACCCTGACTCAATCTTAGAACATCA
  GATTCAAGTAAGACCATTCAACGCATTGAAGACTAAGAATATGAGAAACCTGAATCCAGAAGACATTGAC
  CAGCTCATCACCATCAGCGGCATGGTGATCAGGACATCCCAGCTGATTCCCGAGATGCAGGAGGCCTTCT
  TCCAGTGCCAAGTGTGTGCCCACACGACCCGGGTGGAGATGGACCGCGGCCGCATTGCAGAGCCCAGTGT
  GTGCGGGCGCTGCCACACCACCCACAGCATGGCACTCATCCACAACCGCTCCCTCTTCTCTGACAAGCAG
  ATGATCAAGCTTCAGGAGTCTCCGGAAGACATGCCTGCAGGGCAGACACCACACACAGTTATCCTGTTTG
  CTCACAATGATCTCGTTGACAAGGTCCAGCCTGGGGACAGAGTGAATGTTACAGGCATCTATCGAGCTGT
  GCCTATTCGAGTCAATCCAAGAGTGAGTAATGTGAAGTCTGTCTACAAAACCCACATTGATGTCATTCAT
  TATCGGAAAACGGATGCAAAACGTCTGCATGGCCTTGATGAAGAAGCAGAACAGAAACTTTTTTCAGAGA
  AACGTGTGGAATTGCTTAAGGAACTTTCCAGGAAACCAGACATTTATGAGAGGCTTGCTTCAGCCTTGGC
  TCCAAGCATTTATGAACATGAAGATATAAAGAAGGGAATTTTGCTTCAGCTCTTTGGCGGGACAAGGAAG
  GATTTTAGTCACACTGGAAGGGGCAAATTTCGGGCTGAGATCAACATCTTGCTGTGTGGCGACCCTGGTA
  CCAGCAAGTCCCAGCTGCTGCAGTACGTGTACAACCTCGTCCCCAGGGGCCAGTACACGTCTGGGAAGGG
  CTCCAGTGCAGTTGGCCTCACTGCGTACGTAATGAAAGACCCTGAGACAAGGCAGCTGGTCCTGCAGACA
  GGTGCTCTTGTCCTGAGTGACAACGGCATCTGCTGTATCGATGAGTTCGACAAGATGAATGAAAGTACAA
  GATCGGTATTGCATGAAGTCATGGAACAGCAGACTCTGTCCATTGCAAAGGCTGGGATCATCTGTCAGCT
  CAATGCGCGCACCTCTGTCCTGGCAGCAGCAAATCCCATTGAGTCTCAGTGGAATCCTAAAAAAACAACC
  ATTGAAAACATCCAGCTGCCTCATACTTTATTATCAAGGTTTGATTTGATCTTCCTCTTGCTGGACCCTC
  AGGACGAAGCCTATGACAGGCGTCTGGCTCACCACCTGGTCGCACTGTACTACCAGAGCGAGGAGCAGGC
  AGAGGAGGAGCTCCTGGACATGGCGGTGCTAAAGGACTACATTGCCTACGCGCACAGCACCATCATGCCG
  CGGCTAAGTGAGGAAGCCAGCCAGGCTCTCATCGAGGCTTATGTAGACATGAGGAAGATTGGCAGTAGCC
  GGGGAATGGTTTCTGCATACCCTCGACAGCTAGAGTCATTAATCCGCTTAGCAGAAGCCCATGCTAAAGT
  AAGATTGTCTAACAAAGTTGAAGCCATTGATGTGGAAGAGGCCAAACGCCTCCATCGGGAAGCTCTGAAG
  CAGTCTGCAACTGATCCCCGGACTGGCATCGTGGACATATCTATTCTTACTACGGGGATGAGTGCCACCT
  CTCGTAAACGGAAAGAAGAATTAGCTGAAGCATTGAAAAAGCTTATTTTATCTAAGGGCAAAACACCAGC
  TCTAAAATACCAGCAACTTTTTGAAGATATTCGGGGACAATCTGACATAGCAATTACTAAAGATATGTTT
  GAAGAAGCACTGCGTGCCCTGGCAGATGATGATTTCCTGACAGTGACTGGGAAGACCGTGCGCTTGCTCT
  GA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_182746 unedited
  ACGAGGGCGCTACTCGCCAGGTGGACTCGGNAGTCCGCGAGCGTCGTCGGCAAGCGGCCG
  CCTTTCCACGGTACTCCGAGCACTATGTCGTCCCCGGCGTCGACCCCGAGCCGCCGCGGC
  AGCCGGCGTGGAAGGGCCACCCCCGCCCAGACGCCTCGGAGTGAGGATGCCAGGTCATCT
  CCCTCTCAGAGACGTAGAGGCGAGGATTCCACCTCCACGGGGGAGTTGCAGCCGATGCCA
  ACCTCGCCTGGAGTGGACCTGCAGAGCCCTGCTGCGCAGGACGTGCTGTTTTCCAGCCCT
  CCCCAAATGCATTCTTCAGCTATCCCTCTTGACTTTGATGTTAGTTCACCACTGACATAC
  GGCACTCCCAGCTCTCGGGTAGAGGGAACCCCAAGAAGTGGTGTTAGGGGCACACCTGTG
  AGACAGAGGCCTGACCTGGGCTCTGCACAGAAGGGCCTGCAAGTGGATCTGCAGTCTGAC
  GGGGCAGCAGCAGAAGATATAGTGGCAAGTGAGCAGTCTCTAGGCCAAAAACTTGTGATC
  TGGGGAACAGATGTAAATGTGGCAGCATGCAAAGAAAACTTTCAGAGATTTCTTCAGCGT
  TTTATTGACCCTCTGGCTAAAGAAGAAGAAAATGTTGGCATAGATATTACTGAACCTCTA
  TACATGCAACGACTTGGGGGAGATTAATGGTATTGGTGAGCCATTTTTAAATGTGAACTG
  NGAAACACATCAAATCATTTGACAAAAATTTGTACAGACAACTTATCTTTTACCCACAGG
  AAGNTATTCCAACTTTTGACATGGCTGTCAATGAAATCTTNTTTGANCGGTACCCTGACT
  CAATCTTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_182746
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182746.1, NP_877423.1
• RefSeq Size: 3417 bp
• RefSeq ORF: 2592 bp
• Locus ID: 4173
• UniProt ID: P33991
• Cytogenetics: 8q11.21
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication
• Gene Summary: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks a segment in the 5' UTR, when compared to variant 1. Both variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.