ASF1B (NM_018154) Human Tagged ORF Clone

CAT#: RC206114

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ASF1B (Myc-DDK-tagged)-Human ASF1 anti-silencing function 1 homolog B (S. cerevisiae) (ASF1B)



  "NM_018154" in other vectors (4)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 300.00

USD 450.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ASF1B
Synonyms CIA-II
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206114 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAAGGTGTCGGTGCTGAACGTGGCGGTCCTGGAGAACCCGAGCCCTTTCCACAGCCCCTTCCGGT
TCGAGATCAGCTTCGAGTGCAGTGAAGCCCTGGCGGACGACCTGGAGTGGAAGATCATTTATGTTGGCTC
GGCTGAGAGTGAGGAATTTGATCAGATCCTAGACTCGGTGCTGGTGGGCCCTGTGCCAGCAGGGAGACAC
ATGTTTGTCTTTCAGGCCGACGCCCCCAACCCATCCCTCATCCCAGAGACTGATGCCGTGGGTGTGACTG
TGGTCCTCATCACCTGCACCTACCATGGACAGGAGTTCATCCGAGTGGGCTACTACGTCAACAACGAGTA
CCTCAACCCTGAGCTGCGTGAGAACCCGCCCATGAAGCCAGATTTCTCCCAGCTCCAGCGGAACATCTTG
GCCTCGAACCCCCGGGTGACCCGCTTCCATATCAACTGGGACAACAACATGGACAGGCTGGAGGCCATAG
AGACCCAGGACCCCTCCCTGGGCTGCGGCCTCCCACTCAACTGCACTCCTATCAAGGGCTTGGGGCTCCC
TGGCTGCATCCCTGGCCTCCTCCCTGAGAACTCCATGGACTGCATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206114 protein sequence
Red=Cloning site Green=Tags(s)

MAKVSVLNVAVLENPSPFHSPFRFEISFECSEALADDLEWKIIYVGSAESEEFDQILDSVLVGPVPAGRH
MFVFQADAPNPSLIPETDAVGVTVVLITCTYHGQEFIRVGYYVNNEYLNPELRENPPMKPDFSQLQRNIL
ASNPRVTRFHINWDNNMDRLEAIETQDPSLGCGLPLNCTPIKGLGLPGCIPGLLPENSMDCI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_018154
ORF Size 606 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_018154.3
RefSeq Size 1746 bp
RefSeq ORF 609 bp
Locus ID 55723
UniProt ID Q9NVP2
Cytogenetics 19p13.12
Domains Anti-silence
MW 22.4 kDa
Gene Summary This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. [provided by RefSeq, Jul 2008]

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