MTREX (NM_015360) Human Tagged ORF Clone

CAT#: RC206080

SKIV2L2 (Myc-DDK-tagged)-Human superkiller viralicidic activity 2-like 2 (S. cerevisiae) (SKIV2L2)

Product Images

Circular map for RC206080

• 

  Western blot validation of overexpression lysate (Cat# LY414597) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206080 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MTREX
• Synonyms: Dob1; fSAP118; KIAA0052; Mtr4; SKIV2L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206080 representing NM_015360
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGCATTCGGAGATGAGCTGTTCAGCGTGTTCGAGGGCGACTCGACCACTGCGGCGGGAACCA
  AAAAAGACAAGGAAAAGGACAAGGGGAAATGGAAGGGGCCTCCAGGGTCTGCAGACAAGGCAGGGAAACG
  TTTTGATGGTAAATTACAATCAGAATCAACTAATAATGGAAAAAATAAGAGAGATGTAGATTTCGAAGGT
  ACAGATGAACCCATTTTTGGAAAGAAGCCCAGGATAGAAGAGTCAATAACTGAAGACTTAAGTTTGGCAG
  ACCTGATGCCCAGAGTCAAGGTACAATCAGTTGAAACTGTTGAAGGGTGTACACATGAGGTTGCACTTCC
  TGCAGAAGAGGATTATTTACCACTTAAACCACGAGTTGGAAAAGCTGCTAAGGAATACCCGTTCATTCTT
  GATGCTTTTCAAAGAGAGGCCATTCAGTGTGTTGACAATAATCAGTCTGTTCTAGTATCTGCACATACCT
  CAGCGGGAAAAACAGTATGCGCCGAGTATGCCATTGCATTGGCCTTAAGGGAAAAGCAGCGTGTAATATT
  TACCAGCCCAATTAAGGCTCTGAGTAACCAAAAATACCGTGAAATGTATGAAGAATTTCAAGATGTTGGT
  TTGATGACTGGTGATGTTACTATTAATCCTACGGCATCTTGTCTTGTTATGACCACAGAGATTTTGAGAA
  GTATGCTTTACAGAGGTTCCGAAGTTATGAGAGAAGTTGCTTGGGTTATATTTGATGAAATTCATTATAT
  GAGAGATTCAGAACGTGGTGTAGTATGGGAAGAAACTATTATTTTGCTTCCTGATAACGTCCACTATGTC
  TTTCTTTCGGCTACTATTCCAAATGCCCGACAGTTTGCTGAATGGATTTGCCATTTACATAAACAGCCTT
  GTCATGTTATTTACACAGATTATCGGCCCACTCCATTGCAACACTACATTTTTCCAGCAGGGGGAGATGG
  CCTGCATCTTGTGGTTGATGAAAATGGTGACTTCAGAGAAGATAATTTTAATACTGCAATGCAAGTGCTT
  CGAGATGCAGGTGATTTGGCCAAAGGAGACCAGAAAGGGCGGAAAGGAGGAACAAAAGGACCATCAAATG
  TTTTCAAAATTGTGAAGATGATTATGGAAAGAAATTTCCAACCTGTGATTATTTTCAGTTTTAGTAAGAA
  AGATTGTGAAGCCTATGCACTTCAAATGACCAAATTAGATTTCAACACAGATGAAGAAAAGAAGATGGTT
  GAAGAAGTATTCAGTAATGCAATTGATTGCTTATCCGATGAAGATAAAAAACTCCCTCAGGTAGAACATG
  TACTTCCTCTTTTGAAGAGGGGAATTGGTATTCACCATGGTGGTTTACTTCCTATTTTGAAAGAAACTAT
  AGAAATTCTCTTTTCTGAAGGATTGATAAAGGCCTTATTTGCCACGGAGACCTTTGCTATGGGAATTAAC
  ATGCCAGCTAGAACTGTTTTATTTACAAATGCCCGCAAATTTGATGGGAAGGATTTCCGATGGATTTCTT
  CTGGTGAATACATTCAGATGTCTGGTCGTGCTGGAAGGAGAGGAATGGATGATAGAGGAATTGTAATTCT
  TATGGTAGATGAAAAGATGAGCCCAACAATTGGAAAACAATTACTTAAGGGCTCCGCTGATCCTCTAAAT
  AGTGCTTTCCATTTGACCTACAACATGGTTTTGAACTTACTACGTGTAGAAGAAATTAATCCTGAGTACA
  TGTTGGAAAAATCCTTCTACCAGTTTCAGCATTATAGAGCAATTCCAGGAGTAGTAGAGAAGGTAAAGAA
  TTCAGAAGAACAGTATAATAAAATAGTAATTCCCAATGAAGAAAGTGTGGTTATCTATTATAAGATTAGA
  CAGCAGCTTGCCAAATTGGGTAAAGAAATTGAAGAATATATTCACAAACCAAAATACTGCTTACCTTTTC
  TACAACCAGGTCGTTTGGTAAAGGTAAAGAATGAAGGAGATGACTTTGGCTGGGGAGTAGTGGTGAATTT
  CTCAAAAAAGTCAAATGTTAAGCCTAACTCTGGTGAACTGGATCCTTTGTATGTAGTAGAAGTACTTCTG
  CGCTGTAGCAAAGAGAGCTTGAAAAATTCAGCTACAGAAGCTGCAAAACCAGCTAAACCTGATGAGAAAG
  GAGAGATGCAGGTTGTCCCAGTTTTGGTGCATCTCCTGTCTGCTATCAGCAGTGTTAGGCTTTACATTCC
  TAAAGACCTTCGGCCGGTGGACAATAGACAGAGTGTTTTAAAATCAATACAGGAAGTTCAGAAACGTTTT
  CCTGACGGCATCCCCTTATTAGACCCTATTGATGATATGGGCATTCAAGATCAAGGGCTGAAAAAAGTCA
  TTCAGAAAGTAGAAGCTTTTGAGCATCGAATGTATTCTCATCCACTTCACAATGATCCAAATTTGGAAAC
  TGTGTATACGCTTTGTGAAAAAAAAGCACAGATTGCAATAGATATTAAATCTGCAAAGCGAGAACTGAAG
  AAAGCAAGAACAGTCCTACAAATGGATGAACTCAAATGTCGCAAACGTGTTTTAAGAAGGTTGGGATTTG
  CTACTTCTTCTGATGTAATAGAGATGAAAGGACGAGTGGCTTGTGAGATAAGCAGTGCTGATGAGCTCCT
  TCTAACTGAGATGATGTTTAATGGCCTTTTCAATGACCTTTCTGCAGAACAGGCAACAGCATTATTAAGC
  TGCTTTGTGTTTCAAGAGAATTCTAGTGAGATGCCCAAATTAACAGAACAATTAGCAGGACCACTTCGTC
  AAATGCAGGAATGTGCTAAAAGAATTGCAAAAGTTTCAGCAGAAGCCAAATTGGAAATTGATGAGGAAAC
  TTATCTAAGCTCATTTAAACCTCACTTAATGGATGTAGTATATACCTGGGCAACTGGAGCTACATTTGCC
  CATATCTGCAAAATGACAGATGTCTTTGAAGGCAGCATAATTCGTTGTATGAGGCGCCTGGAAGAATTGC
  TTCGACAAATGTGTCAAGCAGCAAAAGCCATTGGAAACACTGAGCTGGAAAATAAATTTGCAGAAGGAAT
  CACCAAAATCAAGAGAGATATTGTGTTTGCTGCCAGCCTCTACTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206080 representing NM_015360
  Red=Cloning site Green=Tags(s)
  
  MADAFGDELFSVFEGDSTTAAGTKKDKEKDKGKWKGPPGSADKAGKRFDGKLQSESTNNGKNKRDVDFEG
  TDEPIFGKKPRIEESITEDLSLADLMPRVKVQSVETVEGCTHEVALPAEEDYLPLKPRVGKAAKEYPFIL
  DAFQREAIQCVDNNQSVLVSAHTSAGKTVCAEYAIALALREKQRVIFTSPIKALSNQKYREMYEEFQDVG
  LMTGDVTINPTASCLVMTTEILRSMLYRGSEVMREVAWVIFDEIHYMRDSERGVVWEETIILLPDNVHYV
  FLSATIPNARQFAEWICHLHKQPCHVIYTDYRPTPLQHYIFPAGGDGLHLVVDENGDFREDNFNTAMQVL
  RDAGDLAKGDQKGRKGGTKGPSNVFKIVKMIMERNFQPVIIFSFSKKDCEAYALQMTKLDFNTDEEKKMV
  EEVFSNAIDCLSDEDKKLPQVEHVLPLLKRGIGIHHGGLLPILKETIEILFSEGLIKALFATETFAMGIN
  MPARTVLFTNARKFDGKDFRWISSGEYIQMSGRAGRRGMDDRGIVILMVDEKMSPTIGKQLLKGSADPLN
  SAFHLTYNMVLNLLRVEEINPEYMLEKSFYQFQHYRAIPGVVEKVKNSEEQYNKIVIPNEESVVIYYKIR
  QQLAKLGKEIEEYIHKPKYCLPFLQPGRLVKVKNEGDDFGWGVVVNFSKKSNVKPNSGELDPLYVVEVLL
  RCSKESLKNSATEAAKPAKPDEKGEMQVVPVLVHLLSAISSVRLYIPKDLRPVDNRQSVLKSIQEVQKRF
  PDGIPLLDPIDDMGIQDQGLKKVIQKVEAFEHRMYSHPLHNDPNLETVYTLCEKKAQIAIDIKSAKRELK
  KARTVLQMDELKCRKRVLRRLGFATSSDVIEMKGRVACEISSADELLLTEMMFNGLFNDLSAEQATALLS
  CFVFQENSSEMPKLTEQLAGPLRQMQECAKRIAKVSAEAKLEIDEETYLSSFKPHLMDVVYTWATGATFA
  HICKMTDVFEGSIIRCMRRLEELLRQMCQAAKAIGNTELENKFAEGITKIKRDIVFAASLYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015360
• ORF Size: 3126 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015360.5
• RefSeq Size: 4222 bp
• RefSeq ORF: 3129 bp
• Locus ID: 23517
• UniProt ID: P42285
• Cytogenetics: 5q11.2
• Domains: DEAD, helicase_C
• Protein Pathways: RNA degradation
• MW: 117.8 kDa
• Gene Summary: Component of exosome targeting complexes. Subunit of the trimeric nuclear exosome targeting (NEXT) complex, a complex that directs a subset of non-coding short-lived RNAs for exosomal degradation. Subunit of the trimeric poly(A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters (PubMed:27871484). Associated with the RNA exosome complex and involved in the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA (PubMed:17412707, PubMed:29107693). May be involved in pre-mRNA splicing.[UniProtKB/Swiss-Prot Function]