DBPA (YBX3) (NM_003651) Human Tagged ORF Clone

CAT#: RC205856

CSDA (Myc-DDK-tagged)-Human cold shock domain protein A (CSDA), transcript variant 1

Product Images

Circular map for RC205856

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  Western blot validation of overexpression lysate (Cat# LY401208) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205856 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified YBX3 protein (Cat# TP305856). The protein was produced from HEK293T cells transfected with YBX3 cDNA clone (Cat# RC205856) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DBPA
• Synonyms: CSDA; CSDA1; DBPA; ZONAB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205856 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGAGGCGGGCGAGGCCACCACCACCACCACCACCACCCTCCCGCAGGCTCCGACGGAGGCGGCCG
  CCGCGGCTCCCCAGGACCCCGCGCCCAAGAGCCCGGTGGGCAGCGGTGCGCCCCAGGCCGCGGCCCCGGC
  GCCCGCCGCCCACGTCGCAGGAAACCCCGGTGGGGACGCGGCCCCCGCAGCCACGGGCACCGCGGCCGCC
  GCCTCTTTAGCCGCCGCCGCCGGCAGCGAAGACGCGGAGAAAAAAGTTCTCGCCACCAAAGTCCTTGGCA
  CTGTCAAATGGTTCAACGTCAGAAATGGATATGGATTTATAAATCGAAATGACACCAAAGAAGATGTATT
  TGTACATCAGACTGCCATCAAGAAGAATAACCCACGGAAATATCTGCGCAGTGTAGGAGATGGAGAAACT
  GTAGAGTTTGATGTGGTTGAAGGAGAGAAGGGTGCAGAAGCTGCCAATGTGACTGGCCCGGATGGAGTTC
  CTGTGGAAGGGAGTCGTTACGCTGCAGATCGGCGCCGTTACAGACGTGGCTACTATGGAAGGCGCCGTGG
  CCCTCCCCGGAATTACGCTGGGGAGGAGGAGGAGGAAGGGAGCGGCAGCAGTGAAGGATTTGACCCCCCT
  GCCACTGATAGGCAGTTCTCTGGGGCCCGGAATCAGCTGCGCCGCCCCCAGTATCGCCCTCAGTACCGGC
  AGCGGCGGTTCCCGCCTTACCACGTGGGACAGACCTTTGACCGTCGCTCACGGGTCTTACCCCATCCCAA
  CAGAATACAGGCTGGTGAGATTGGAGAGATGAAGGATGGAGTCCCAGAGGGAGCACAACTTCAGGGACCG
  GTTCATCGAAATCCAACTTACCGCCCAAGGTACCGTAGCAGGGGACCTCCTCGCCCACGACCTGCCCCAG
  CAGTTGGAGAGGCTGAAGATAAAGAAAATCAGCAAGCCACCAGTGGTCCAAACCAGCCGTCTGTTCGCCG
  TGGATATCGGCGTCCCTACAATTACCGGCGTCGCCCGCGTCCTCCTAACGCTCCTTCACAAGATGGCAAA
  GAGGCCAAGGCAGGTGAAGCACCAACTGAGAACCCTGCTCCACCCACCCAGCAGAGCAGTGCTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205856 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSEAGEATTTTTTTLPQAPTEAAAAAPQDPAPKSPVGSGAPQAAAPAPAAHVAGNPGGDAAPAATGTAAA
  ASLAAAAGSEDAEKKVLATKVLGTVKWFNVRNGYGFINRNDTKEDVFVHQTAIKKNNPRKYLRSVGDGET
  VEFDVVEGEKGAEAANVTGPDGVPVEGSRYAADRRRYRRGYYGRRRGPPRNYAGEEEEEGSGSSEGFDPP
  ATDRQFSGARNQLRRPQYRPQYRQRRFPPYHVGQTFDRRSRVLPHPNRIQAGEIGEMKDGVPEGAQLQGP
  VHRNPTYRPRYRSRGPPRPRPAPAVGEAEDKENQQATSGPNQPSVRRGYRRPYNYRRRPRPPNAPSQDGK
  EAKAGEAPTENPAPPTQQSSAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003651
• ORF Size: 1116 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003651.3, NP_003642.2
• RefSeq Size: 1976 bp
• RefSeq ORF: 1119 bp
• Locus ID: 8531
• UniProt ID: P16989
• Cytogenetics: 12p13.2
• Domains: CSD
• Protein Families: Transcription Factors
• Protein Pathways: Tight junction
• MW: 40.1 kDa
• Gene Summary: Binds to the GM-CSF promoter. Seems to act as a repressor. Binds also to full-length mRNA and to short RNA sequences containing the consensus site 5'-UCCAUCA-3'. May have a role in translation repression (By similarity).[UniProtKB/Swiss-Prot Function]