STAC (NM_003149) Human Tagged ORF Clone

CAT#: RC205851

STAC (Myc-DDK-tagged)-Human SH3 and cysteine rich domain (STAC)

Product Images

Circular map for RC205851

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  Western blot validation of overexpression lysate (Cat# LY401094) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205851 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified STAC protein (Cat# TP305851). The protein was produced from HEK293T cells transfected with STAC cDNA clone (Cat# RC205851) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STAC
• Synonyms: STAC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205851 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCCTCCGAGCAGCCCCCGCGAGGACGGCGTGGACGGGCTGCCCAAGGAGGCGGTGGGCGCCGAGC
  AACCGCCCTCTCCTGCATCCACCAGCAGCCAGGAATCCAAGCTCCAGAAACTAAAACGATCACTTTCTTT
  CAAGACCAAGAGTTTACGGAGCAAAAGTGCTGACAACTTCTTCCAGCGAACCAACAGCGAAGACATGAAA
  CTGCAAGCACACATGGTGGCTGAGATCAGCCCCAGCTCCAGCCCACTCCCTGCTCCAGGAAGCCTGACGT
  CCACACCCGCCAGGGCTGGTCTGCATCCAGGTGGCAAGGCTCATGCCTTTCATGAATACATCTTCAAGAA
  GCCCACTTTCTGTGATGTCTGCAACCACATGATAGTGGGAACAAATGCTAAGCATGGACTGCGCTGCAAA
  GCCTGTAAGATGAGCATCCACCACAAGTGCACAGATGGCCTGGCACCCCAGCGGTGCATGGGCAAGCTGC
  CAAAGGGGTTTCGGCGTTACTACAGCTCCCCCTTGCTCATTCATGAACAGTTTGGCTGCATTAAAGAAGT
  TATGCCCATTGCCTGTGGCAATAAGGTGGACCCTGTCTACGAGACCCTCCGCTTCGGCACCTCCCTGGCC
  CAGAGGACAAAGAAGGGCAGCTCCGGCAGTGGCTCTGACTCACCTCACAGAACCTCTACTTCAGATCTTG
  TGGAGGTTCCTGAGGAAGCCAATGGGCCAGGAGGCGGGTATGACCTAAGGAAACGCAGCAACAGCGTGTT
  TACATATCCAGAAAATGGCACTGATGATTTCAGAGATCCAGCGAAGAACATAAACCACCAGGGATCTCTT
  TCCAAAGACCCATTACAGATGAACACCTATGTTGCCTTGTACAAATTTGTACCACAGGAGAATGAAGATT
  TGGAAATGAGGCCAGGAGACATAATTACTCTTTTAGAGGATTCCAATGAAGACTGGTGGAAAGGGAAAAT
  TCAAGACAGAATTGGCTTCTTTCCAGCCAACTTTGTTCAGAGACTACAACAAAATGAGAAGATTTTTAGA
  TGTGTTAGAACCTTCATTGGGTGTAAGGAACAGGGGCAGATAACACTGAAAGAGAATCAGATCTGCGTGA
  GTTCTGAAGAAGAACAAGATGGTTTTATCAGAGTCCTCAGTGGAAAAAAGAAAGGCCTCATCCCCCTTGA
  TGTACTAGAAAACATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205851 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MIPPSSPREDGVDGLPKEAVGAEQPPSPASTSSQESKLQKLKRSLSFKTKSLRSKSADNFFQRTNSEDMK
  LQAHMVAEISPSSSPLPAPGSLTSTPARAGLHPGGKAHAFHEYIFKKPTFCDVCNHMIVGTNAKHGLRCK
  ACKMSIHHKCTDGLAPQRCMGKLPKGFRRYYSSPLLIHEQFGCIKEVMPIACGNKVDPVYETLRFGTSLA
  QRTKKGSSGSGSDSPHRTSTSDLVEVPEEANGPGGGYDLRKRSNSVFTYPENGTDDFRDPAKNINHQGSL
  SKDPLQMNTYVALYKFVPQENEDLEMRPGDIITLLEDSNEDWWKGKIQDRIGFFPANFVQRLQQNEKIFR
  CVRTFIGCKEQGQITLKENQICVSSEEEQDGFIRVLSGKKKGLIPLDVLENI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003149
• ORF Size: 1206 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003149.2
• RefSeq Size: 3098 bp
• RefSeq ORF: 1209 bp
• Locus ID: 6769
• UniProt ID: Q99469
• Cytogenetics: 3p22.3-p22.2
• Domains: SH3, DAG_PE-bind
• MW: 44.6 kDa
• Gene Summary: Promotes expression of the ion channel CACNA1H at the cell membrane, and thereby contributes to the regulation of channel activity. Plays a minor and redundant role in promoting the expression of calcium channel CACNA1S at the cell membrane, and thereby contributes to increased channel activity. Slows down the inactivation rate of the calcium channel CACNA1C.[UniProtKB/Swiss-Prot Function]