FUCA1 (NM_000147) Human Tagged ORF Clone

CAT#: RC205683

FUCA1 (Myc-DDK-tagged)-Human fucosidase, alpha-L- 1, tissue (FUCA1)

Product Images

Circular map for RC205683

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  Western blot validation of overexpression lysate (Cat# LY424897) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205683 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FUCA1 protein (Cat# TP305683). The protein was produced from HEK293T cells transfected with FUCA1 cDNA clone (Cat# RC205683) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FUCA1
• Synonyms: FUCA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205683 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTCGCGGCCGGCGGGTCCCGCGCTGTTGCTGCTGCTGCTCTTCCTCGGAGCGGCCGAGTCGGTGC
  GTCGGGCCCAGCCTCCGCGCCGCTACACCCCAGACTGGCCGAGCCTGGATTCTCGGCCGCTGCCGGCCTG
  GTTCGACGAAGCCAAGTTCGGGGTGTTCATCCACTGGGGCGTGTTCTCGGTGCCCGCCTGGGGCAGCGAG
  TGGTTCTGGTGGCACTGGCAGGGCGAGGGGCGGCCGCAGTACCAGCGCTTCATGCGCGACAACTACCCGC
  CCGGCTTCAGCTACGCCGACTTCGGACCGCAGTTCACTGCGCGCTTCTTCCACCCGGAGGAGTGGGCCGA
  CCTCTTCCAGGCCGCGGGCGCCAAGTATGTAGTTTTGACGACAAAGCATCACGAAGGCTTCACAAACTGG
  CCGAGTCCTGTGTCTTGGAACTGGAACTCCAAAGACGTGGGGCCTCATCGGGATTTGGTTGGTGAATTGG
  GAACAGCTCTCCGGAAGAGGAACATCCGCTATGGACTATACCACTCACTCTTAGAGTGGTTCCATCCACT
  CTATCTACTTGATAAGAAAAATGGCTTCAAAACACAGCATTTTGTCAGTGCAAAAACAATGCCAGAGCTG
  TACGACCTTGTTAACAGCTATAAACCTGATCTGATCTGGTCTGATGGGGAGTGGGAATGTCCTGATACTT
  ACTGGAACTCCACAAATTTTCTTTCATGGCTCTACAATGACAGCCCTGTCAAGGATGAGGTGGTAGTAAA
  TGACCGATGGGGTCAGAACTGTTCCTGTCACCATGGAGGATACTATAACTGTGAAGATAAATTCAAGCCA
  CAGAGCTTGCCAGATCACAAGTGGGAGATGTGCACCAGCATTGACAAGTTTTCCTGGGGCTATCGTCGTG
  ACATGGCATTGTCTGATGTTACAGAAGAATCTGAAATCATTTCGGAACTGGTTCAGACAGTAAGTTTGGG
  AGGCAACTATCTTCTGAACATTGGACCAACTAAAGATGGACTGATTGTTCCCATCTTCCAAGAAAGGCTT
  CTTGCTGTTGGGAAATGGCTGAGCATCAATGGGGAGGCTATCTATGCCTCCAAACCATGGCGGGTGCAAT
  GGGAAAAGAACACAACATCTGTATGGTATACCTCAAAGGGATCGGCTGTTTATGCCATTTTTCTGCACTG
  GCCAGAAAATGGAGTCTTAAACCTTGAATCCCCCATAACTACCTCAACTACAAAGATAACAATGCTGGGA
  ATTCAAGGAGATCTGAAGTGGTCCACAGATCCAGATAAAGGTCTCTTCATCTCTCTACCCCAGTTGCCAC
  CCTCTGCTGTCCCCGCAGAGTTTGCTTGGACTATAAAGCTGACAGGAGTGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205683 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRSRPAGPALLLLLLFLGAAESVRRAQPPRRYTPDWPSLDSRPLPAWFDEAKFGVFIHWGVFSVPAWGSE
  WFWWHWQGEGRPQYQRFMRDNYPPGFSYADFGPQFTARFFHPEEWADLFQAAGAKYVVLTTKHHEGFTNW
  PSPVSWNWNSKDVGPHRDLVGELGTALRKRNIRYGLYHSLLEWFHPLYLLDKKNGFKTQHFVSAKTMPEL
  YDLVNSYKPDLIWSDGEWECPDTYWNSTNFLSWLYNDSPVKDEVVVNDRWGQNCSCHHGGYYNCEDKFKP
  QSLPDHKWEMCTSIDKFSWGYRRDMALSDVTEESEIISELVQTVSLGGNYLLNIGPTKDGLIVPIFQERL
  LAVGKWLSINGEAIYASKPWRVQWEKNTTSVWYTSKGSAVYAIFLHWPENGVLNLESPITTSTTKITMLG
  IQGDLKWSTDPDKGLFISLPQLPPSAVPAEFAWTIKLTGVK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000147
• ORF Size: 1383 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000147.5
• RefSeq Size: 2133 bp
• RefSeq ORF: 1401 bp
• Locus ID: 2517
• UniProt ID: P04066
• Cytogenetics: 1p36.11
• Domains: Alpha_L_fucos
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome, Other glycan degradation
• MW: 53.2 kDa
• Gene Summary: The protein encoded by this gene is a lysosomal enzyme involved in the degradation of fucose-containing glycoproteins and glycolipids. Mutations in this gene are associated with fucosidosis (FUCA1D), which is an autosomal recessive lysosomal storage disease. A pseudogene of this locus is present on chr 2.[provided by RefSeq, Oct 2009]