HACE1 (NM_020771) Human Tagged ORF Clone

CAT#: RC205602

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HACE1 (Myc-DDK-tagged)-Human HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (HACE1)



  "NM_020771" in other vectors (6)

Reconstitution Protocol

USD 832.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol HACE1
Synonyms SPPRS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205602 representing NM_020771
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAGAGCGATGGAGCAACTCAACCGCCTGACGCGCTCGCTGCGCCGCGCGCGCACCGTGGAGTTGC
CCGAGGATAATGAAACTGCTGTTTATACATTAATGCCAATGGTTATGGCTGATCAACACAGGTCTGTTTC
TGAACTACTATCAAATTCAAAATTTGATGTCAATTATGCATTCGGACGTGTGAAAAGAAGCTTGCTTCAC
ATTGCAGCAAATTGTGGATCGGTGGAATGCTTGGTTTTGCTGTTAAAGAAAGGAGCAAATCCTAACTATC
AAGATATTTCAGGCTGTACACCCCTTCATTTGGCAGCAAGAAATGGGCAGAAGAAATGTATGAGTAAATT
ATTAGAATATAGCGCTGATGTCAACATTTGTAATAATGAAGGCCTTACAGCAATACATTGGCTGGCTGTG
AATGGGCGGACAGAACTACTCCATGACCTTGTGCAGCATGTCAGTGATGTTGATGTTGAGGATGCCATGG
GGCAGACAGCACTGCATGTTGCCTGCCAGAACGGTCACAAGACGACAGTGCAGTGCTTGCTAGACAGTGG
TGCTGATATTAACAGGCCAAATGTATCAGGAGCAACTCCATTGTACTTTGCTTGCAGTCATGGTCAGAGA
GATACAGCACAGATCCTACTATTACGAGGAGCCAAATATCTGCCAGATAAAAATGGAGTAACTCCTCTGG
ATTTATGTGTACAGGGTGGATATGGAGAGACTTGTGAAGTATTAATTCAATATCACCCGAGGCTTTTTCA
GACTATTATTCAAATGACACAGAATGAAGACCTCCGAGAAAACATGTTACGGCAAGTTCTGGAGCATTTG
TCTCAGCAAAGTGAAAGCCAGTACCTAAAGATTCTAACAAGCCTTGCTGAAGTTGCTACAACAAATGGTC
ATAAACTGCTTAGCCTCTCTAGCAATTATGATGCTCAAATGAAGAGCCTTTTAAGGATTGTGAGAATGTT
TTGTCACGTCTTTCGAATTGGTCCATCCTCCCCCAGTAATGGAATTGATATGGGCTACAATGGGAATAAA
ACTCCAAGAAGCCAGGTGTTCAAGCCTCTGGAATTGCTTTGGCACTCGTTAGATGAATGGCTAGTTTTAA
TAGCCACAGAATTGATGAAAAACAAAAGAGACTCAACAGAGATCACTTCTATTTTACTGAAACAAAAAGG
CCAAGATCAAGATGCTGCTTCCATTCCTCCATTTGAACCTCCAGGACCTGGGAGCTATGAAAATCTGTCC
ACTGGCACAAGGGAATCTAAACCAGATGCTCTTGCAGGGAGACAGGAAGCCAGTGCAGATTGTCAGGATG
TTATTTCTATGACAGCTAACCGGCTAAGTGCTGTCATTCAAGCTTTTTACATGTGCTGTTCTTGTCAGAT
GCCTCCGGGAATGACTTCACCTCGTTTCATTGAATTTGTCTGCAAACATGATGAAGTTTTAAAATGCTTT
GTTAATAGAAATCCCAAAATTATATTTGACCACTTTCACTTTCTCCTTGAATGTCCTGAGTTGATGTCAA
GATTCATGCATATCATAAAAGCACAGCCTTTTAAAGATCGCTGTGAATGGTTCTATGAACATTTGCATTC
AGGACAGCCAGATTCAGATATGGTGCACAGGCCAGTGAATGAAAATGATATCCTGCTGGTTCACAGAGAT
TCTATTTTTAGGAGTAGCTGTGAAGTTGTGTCAAAAGCAAATTGTGCAAAGCTAAAGCAAGGGATTGCTG
TACGGTTCCATGGAGAAGAAGGCATGGGTCAAGGTGTTGTGCGTGAGTGGTTTGATATTCTGTCCAATGA
GATAGTCAATCCTGATTATGCATTGTTTACCCAGTCAGCTGATGGAACAACTTTTCAGCCTAATAGCAAC
TCTTATGTAAATCCTGATCACTTGAACTATTTTCGGTTTGCTGGGCAGATCTTGGGATTAGCGTTGAACC
ACAGGCAGCTGGTCAATATTTACTTCACACGATCCTTCTACAAGCACATTCTTGGTATTCCTGTAAATTA
CCAAGATGTGGCATCCATTGATCCAGAATATGCGAAAAATTTGCAATGGATTTTAGATAATGATATAAGT
GATCTGGGTCTAGAACTAACTTTTTCTGTTGAGACTGATGTGTTTGGAGCAATGGAAGAGGTGCCTTTGA
AACCTGGGGGTGGGAGTATTCTTGTGACACAAAATAATAAAGCGGAGTACGTCCAGCTTGTTACTGAACT
TCGAATGACAAGAGCCATTCAGCCTCAGATCAATGCTTTTTTACAGGGCTTTCATATGTTCATTCCACCC
TCCCTCATACAGCTTTTTGATGAATATGAATTGGAGCTACTGCTTTCTGGCATGCCAGAAATTGATGTGA
GTGATTGGATAAAAAATACAGAATACACAAGTGGCTATGAAAGAGAAGATCCAGTTATTCAGTGGTTCTG
GGAAGTTGTAGAAGACATTACTCAAGAGGAGAGAGTTCTTCTCTTACAGTTTGTTACGGGCAGTTCCAGG
GTCCCACATGGTGGGTTTGCTAATATCATGGGTGGAAGTGGATTGCAAAACTTTACAATCGCTGCTGTGC
CATATACTCCAAATCTTTTACCAACTTCAAGCACATGCATCAACATGCTCAAGTTACCTGAATACCCAAG
TAAAGAAATACTCAAGGACAGACTTCTTGTGGCACTACATTGTGGCAGCTATGGTTACACAATGGCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205602 representing NM_020771
Red=Cloning site Green=Tags(s)

MERAMEQLNRLTRSLRRARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLH
IAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAV
NGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQR
DTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHL
SQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNK
TPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLS
TGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCF
VNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRD
SIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSN
SYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDIS
DLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPP
SLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSR
VPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_020771
ORF Size 2727 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_020771.4
RefSeq Size 4576 bp
RefSeq ORF 2730 bp
Locus ID 57531
UniProt ID Q8IYU2
Cytogenetics 6q16.3
Protein Families Druggable Genome
MW 102.2 kDa
Gene Summary This gene encodes a HECT domain and ankyrin repeat-containing ubiquitin ligase. The encoded protein is involved in specific tagging of target proteins, leading to their subcellular localization or proteasomal degradation. The protein is a potential tumor suppressor and is involved in the pathophysiology of several tumors, including Wilm's tumor. [provided by RefSeq, Mar 2016]

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