HACE1 (NM_020771) Human Tagged ORF Clone

CAT#: RC205602

HACE1 (Myc-DDK-tagged)-Human HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (HACE1)

Product Images

Circular map for RC205602

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HACE1 (Cat# RC205602, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HACE1 (Cat# TA810060)(1:500). Positive lysates LY412326 (100ug) and LC412326 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY412326) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205602 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified HACE1 protein (Cat# TP305602). The protein was produced from HEK293T cells transfected with HACE1 cDNA clone (Cat# RC205602) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HACE1
• Synonyms: SPPRS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205602 representing NM_020771
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGAGCGATGGAGCAACTCAACCGCCTGACGCGCTCGCTGCGCCGCGCGCGCACCGTGGAGTTGC
  CCGAGGATAATGAAACTGCTGTTTATACATTAATGCCAATGGTTATGGCTGATCAACACAGGTCTGTTTC
  TGAACTACTATCAAATTCAAAATTTGATGTCAATTATGCATTCGGACGTGTGAAAAGAAGCTTGCTTCAC
  ATTGCAGCAAATTGTGGATCGGTGGAATGCTTGGTTTTGCTGTTAAAGAAAGGAGCAAATCCTAACTATC
  AAGATATTTCAGGCTGTACACCCCTTCATTTGGCAGCAAGAAATGGGCAGAAGAAATGTATGAGTAAATT
  ATTAGAATATAGCGCTGATGTCAACATTTGTAATAATGAAGGCCTTACAGCAATACATTGGCTGGCTGTG
  AATGGGCGGACAGAACTACTCCATGACCTTGTGCAGCATGTCAGTGATGTTGATGTTGAGGATGCCATGG
  GGCAGACAGCACTGCATGTTGCCTGCCAGAACGGTCACAAGACGACAGTGCAGTGCTTGCTAGACAGTGG
  TGCTGATATTAACAGGCCAAATGTATCAGGAGCAACTCCATTGTACTTTGCTTGCAGTCATGGTCAGAGA
  GATACAGCACAGATCCTACTATTACGAGGAGCCAAATATCTGCCAGATAAAAATGGAGTAACTCCTCTGG
  ATTTATGTGTACAGGGTGGATATGGAGAGACTTGTGAAGTATTAATTCAATATCACCCGAGGCTTTTTCA
  GACTATTATTCAAATGACACAGAATGAAGACCTCCGAGAAAACATGTTACGGCAAGTTCTGGAGCATTTG
  TCTCAGCAAAGTGAAAGCCAGTACCTAAAGATTCTAACAAGCCTTGCTGAAGTTGCTACAACAAATGGTC
  ATAAACTGCTTAGCCTCTCTAGCAATTATGATGCTCAAATGAAGAGCCTTTTAAGGATTGTGAGAATGTT
  TTGTCACGTCTTTCGAATTGGTCCATCCTCCCCCAGTAATGGAATTGATATGGGCTACAATGGGAATAAA
  ACTCCAAGAAGCCAGGTGTTCAAGCCTCTGGAATTGCTTTGGCACTCGTTAGATGAATGGCTAGTTTTAA
  TAGCCACAGAATTGATGAAAAACAAAAGAGACTCAACAGAGATCACTTCTATTTTACTGAAACAAAAAGG
  CCAAGATCAAGATGCTGCTTCCATTCCTCCATTTGAACCTCCAGGACCTGGGAGCTATGAAAATCTGTCC
  ACTGGCACAAGGGAATCTAAACCAGATGCTCTTGCAGGGAGACAGGAAGCCAGTGCAGATTGTCAGGATG
  TTATTTCTATGACAGCTAACCGGCTAAGTGCTGTCATTCAAGCTTTTTACATGTGCTGTTCTTGTCAGAT
  GCCTCCGGGAATGACTTCACCTCGTTTCATTGAATTTGTCTGCAAACATGATGAAGTTTTAAAATGCTTT
  GTTAATAGAAATCCCAAAATTATATTTGACCACTTTCACTTTCTCCTTGAATGTCCTGAGTTGATGTCAA
  GATTCATGCATATCATAAAAGCACAGCCTTTTAAAGATCGCTGTGAATGGTTCTATGAACATTTGCATTC
  AGGACAGCCAGATTCAGATATGGTGCACAGGCCAGTGAATGAAAATGATATCCTGCTGGTTCACAGAGAT
  TCTATTTTTAGGAGTAGCTGTGAAGTTGTGTCAAAAGCAAATTGTGCAAAGCTAAAGCAAGGGATTGCTG
  TACGGTTCCATGGAGAAGAAGGCATGGGTCAAGGTGTTGTGCGTGAGTGGTTTGATATTCTGTCCAATGA
  GATAGTCAATCCTGATTATGCATTGTTTACCCAGTCAGCTGATGGAACAACTTTTCAGCCTAATAGCAAC
  TCTTATGTAAATCCTGATCACTTGAACTATTTTCGGTTTGCTGGGCAGATCTTGGGATTAGCGTTGAACC
  ACAGGCAGCTGGTCAATATTTACTTCACACGATCCTTCTACAAGCACATTCTTGGTATTCCTGTAAATTA
  CCAAGATGTGGCATCCATTGATCCAGAATATGCGAAAAATTTGCAATGGATTTTAGATAATGATATAAGT
  GATCTGGGTCTAGAACTAACTTTTTCTGTTGAGACTGATGTGTTTGGAGCAATGGAAGAGGTGCCTTTGA
  AACCTGGGGGTGGGAGTATTCTTGTGACACAAAATAATAAAGCGGAGTACGTCCAGCTTGTTACTGAACT
  TCGAATGACAAGAGCCATTCAGCCTCAGATCAATGCTTTTTTACAGGGCTTTCATATGTTCATTCCACCC
  TCCCTCATACAGCTTTTTGATGAATATGAATTGGAGCTACTGCTTTCTGGCATGCCAGAAATTGATGTGA
  GTGATTGGATAAAAAATACAGAATACACAAGTGGCTATGAAAGAGAAGATCCAGTTATTCAGTGGTTCTG
  GGAAGTTGTAGAAGACATTACTCAAGAGGAGAGAGTTCTTCTCTTACAGTTTGTTACGGGCAGTTCCAGG
  GTCCCACATGGTGGGTTTGCTAATATCATGGGTGGAAGTGGATTGCAAAACTTTACAATCGCTGCTGTGC
  CATATACTCCAAATCTTTTACCAACTTCAAGCACATGCATCAACATGCTCAAGTTACCTGAATACCCAAG
  TAAAGAAATACTCAAGGACAGACTTCTTGTGGCACTACATTGTGGCAGCTATGGTTACACAATGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205602 representing NM_020771
  Red=Cloning site Green=Tags(s)
  
  MERAMEQLNRLTRSLRRARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLH
  IAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAV
  NGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQR
  DTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHL
  SQQSESQYLKILTSLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRMFCHVFRIGPSSPSNGIDMGYNGNK
  TPRSQVFKPLELLWHSLDEWLVLIATELMKNKRDSTEITSILLKQKGQDQDAASIPPFEPPGPGSYENLS
  TGTRESKPDALAGRQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCF
  VNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVNENDILLVHRD
  SIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSN
  SYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDIS
  DLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPP
  SLIQLFDEYELELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSR
  VPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020771
• ORF Size: 2727 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020771.4
• RefSeq Size: 4576 bp
• RefSeq ORF: 2730 bp
• Locus ID: 57531
• UniProt ID: Q8IYU2
• Cytogenetics: 6q16.3
• Protein Families: Druggable Genome
• MW: 102.2 kDa
• Gene Summary: This gene encodes a HECT domain and ankyrin repeat-containing ubiquitin ligase. The encoded protein is involved in specific tagging of target proteins, leading to their subcellular localization or proteasomal degradation. The protein is a potential tumor suppressor and is involved in the pathophysiology of several tumors, including Wilm's tumor. [provided by RefSeq, Mar 2016]