ACRBP (NM_032489) Human Tagged ORF Clone

CAT#: RC205415

ACRBP (Myc-DDK-tagged)-Human acrosin binding protein (ACRBP)

Product Images

Circular map for RC205415

• 

  Western blot validation of overexpression lysate (Cat# LY403164) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205415 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACRBP
• Synonyms: CT23; OY-TES-1; SP32
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205415 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAAGCCAGCCGCTGGCTTCCTTCCCTCACTCCTGAAGGTGCTGCTCCTGCCTCTGGCACCTGCCG
  CAGCCCAGGATTCGACTCAGGCCTCCACTCCAGGCAGCCCTCTCTCTCCTACCGAATACGAACGCTTCTT
  CGCACTGCTGACTCCAACCTGGAAGGCAGAGACTACCTGCCGTCTCCGTGCAACCCACGGCTGCCGGAAT
  CCCACACTCGTCCAGCTGGACCAATATGAAAACCACGGCTTAGTGCCCGATGGTGCTGTCTGCTCCAACC
  TCCCTTATGCCTCCTGGTTTGAGTCTTTCTGCCAGTTCACTCACTACCGTTGCTCCAACCACGTCTACTA
  TGCCAAGAGAGTCCTGTGTTCCCAGCCAGTCTCTATTCTCTCACCTAACACTCTCAAGGAGATAGAAGCT
  TCAGCTGAAGTCTCACCCACCACGATGACCTCCCCCATCTCACCCCACTTCACAGTGACAGAACGCCAGA
  CCTTCCAGCCCTGGCCTGAGAGGCTCAGCAACAACGTGGAAGAGCTCCTACAATCCTCCTTGTCCCTGGG
  AGGCCAGGAGCAAGCGCCAGAGCACAAGCAGGAGCAAGGAGTGGAGCACAGGCAGGAGCCGACACAAGAA
  CACAAGCAGGAAGAGGGGCAGAAACAGGAAGAGCAAGAAGAGGAACAGGAAGAGGAGGGAAAGCAGGAAG
  AAGGACAGGGGACTAAGGAGGGACGGGAGGCTGTGTCTCAGCTGCAGACAGACTCAGAGCCCAAGTTTCA
  CTCTGAATCTCTATCTTCTAACCCTTCCTCTTTTGCTCCCCGGGTACGAGAAGTAGAGTCTACTCCTATG
  ATAATGGAGAACATCCAGGAGCTCATTCGATCAGCCCAGGAAATAGATGAAATGAATGAAATATATGATG
  AGAACTCCTACTGGAGAAACCAAAACCCTGGCAGCCTCCTGCAGCTGCCCCACACAGAGGCCTTGCTGGT
  GCTGTGCTATTCGATCGTGGAGAATACCTGCATCATAACCCCCACAGCCAAGGCCTGGAAGTACATGGAG
  GAGGAGATCCTTGGTTTCGGGAAGTCGGTCTGTGACAGCCTTGGGCGGCGACACATGTCTACCTGTGCCC
  TCTGTGACTTCTGCTCCTTGAAGCTGGAGCAGTGCCACTCAGAGGCCAGCCTGCAGCGGCAACAATGCGA
  CACCTCCCACAAGACTCCCTTTGTCAGCCCCTTGCTTGCCTCCCAGAGCCTGTCCATCGGCAACCAGGTA
  GGGTCCCCAGAATCAGGCCGCTTTTACGGGCTGGATTTGTACGGTGGGCTCCACATGGACTTCTGGTGTG
  CCCGGCTTGCCACGAAAGGCTGTGAAGATGTCCGAGTCTCTGGGTGGCTCCAGACTGAGTTCCTTAGCTT
  CCAGGATGGGGATTTCCCTACCAAGATTTGTGACACAGACTATATCCAGTACCCAAACTACTGTTCCTTC
  AAAAGCCAGCAGTGTCTGATGAGAAACCGCAATCGGAAGGTGTCCCGCATGAGATGTCTGCAGAATGAGA
  CTTACAGTGCGCTGAGCCCTGGCAAAAGTGAGGACGTTGTGCTTCGATGGAGCCAGGAGTTCAGCACCTT
  GACTCTAGGCCAGTTCGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205415 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRKPAAGFLPSLLKVLLLPLAPAAAQDSTQASTPGSPLSPTEYERFFALLTPTWKAETTCRLRATHGCRN
  PTLVQLDQYENHGLVPDGAVCSNLPYASWFESFCQFTHYRCSNHVYYAKRVLCSQPVSILSPNTLKEIEA
  SAEVSPTTMTSPISPHFTVTERQTFQPWPERLSNNVEELLQSSLSLGGQEQAPEHKQEQGVEHRQEPTQE
  HKQEEGQKQEEQEEEQEEEGKQEEGQGTKEGREAVSQLQTDSEPKFHSESLSSNPSSFAPRVREVESTPM
  IMENIQELIRSAQEIDEMNEIYDENSYWRNQNPGSLLQLPHTEALLVLCYSIVENTCIITPTAKAWKYME
  EEILGFGKSVCDSLGRRHMSTCALCDFCSLKLEQCHSEASLQRQQCDTSHKTPFVSPLLASQSLSIGNQV
  GSPESGRFYGLDLYGGLHMDFWCARLATKGCEDVRVSGWLQTEFLSFQDGDFPTKICDTDYIQYPNYCSF
  KSQQCLMRNRNRKVSRMRCLQNETYSALSPGKSEDVVLRWSQEFSTLTLGQFG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032489
• ORF Size: 1629 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032489.3
• RefSeq Size: 1886 bp
• RefSeq ORF: 1632 bp
• Locus ID: 84519
• UniProt ID: Q8NEB7
• Cytogenetics: 12p13.31
• Protein Families: Secreted Protein
• MW: 61.4 kDa
• Gene Summary: The protein encoded by this gene is similar to proacrosin binding protein sp32 precursor found in mouse, guinea pig, and pig. This protein is located in the sperm acrosome and is thought to function as a binding protein to proacrosin for packaging and condensation of the acrosin zymogen in the acrosomal matrix. This protein is a member of the cancer/testis family of antigens and it is found to be immunogenic. In normal tissues, this mRNA is expressed only in testis, whereas it is detected in a range of different tumor types such as bladder, breast, lung, liver, and colon. [provided by RefSeq, Jul 2008]