CH25H (NM_003956) Human Tagged ORF Clone

CAT#: RC204716

  • TrueORF®

CH25H (Myc-DDK-tagged)-Human cholesterol 25-hydroxylase (CH25H)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro

AAV Particle: DDK


  "NM_003956" in other vectors (6)

Reconstitution Protocol

USD 300.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

USD 198.00


Rabbit Polyclonal Anti-CH25H Antibody
    • 100 ul

USD 539.00

Other products for "CH25H"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CH25H
Synonyms C25H
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC204716 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTGCCACAACTGCTCCGACCCCCAGGTCCTTTGCAGCTCCGGGCAGCTGTTCCTGCAGCCCCTCT
GGGACCACCTGAGGAGCTGGGAGGCCCTCCTACAGTCGCCCTTCTTCCCGGTCATCTTCTCCATCACCAC
ATACGTGGGCTTTTGCCTGCCCTTCGTGGTCCTGGATATCCTGTGCTCCTGGGTGCCCGCCCTGCGGCGC
TACAAGATCCACCCTGACTTCTCGCCATCCGCGCAGCAGCTGCTACCTTGCCTGGGGCAGACCCTCTACC
AGCATGTGATGTTTGTGTTCCCCGTGACGCTGCTGCATTGGGCCCGCAGCCCGGCCCTCCTGCCCCACGA
AGCTCCCGAGCTGCTCCTGCTGCTGCACCACATCCTGTTCTGCCTGCTACTCTTCGACATGGAGTTCTTC
GTGTGGCACCTGCTGCACCACAAGGTGCCCTGGCTGTACCGCACCTTCCACAAGGTGCACCACCAGAACT
CGTCCTCGTTCGCGCTGGCAACGCAGTATATGAGCGTCTGGGAACTGTTTTCTTTGGGCTTCTTCGACAT
GATGAACGTCACACTGCTCGGGTGCCACCCGCTCACCACCCTGACCTTCCACGTGGTCAACATCTGGCTT
TCCGTGGAGGACCACTCCGGCTACAACTTCCCTTGGTCCACTCACAGACTGGTGCCCTTCGGGTGGTACG
GGGGTGTGGTGCACCACGACCTGCATCACTCTCACTTTAACTGCAACTTCGCTCCGTACTTTACACACTG
GGACAAAATACTGGGAACGCTGCGGACTGCATCTGTCCCAGCGCGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204716 protein sequence
Red=Cloning site Green=Tags(s)

MSCHNCSDPQVLCSSGQLFLQPLWDHLRSWEALLQSPFFPVIFSITTYVGFCLPFVVLDILCSWVPALRR
YKIHPDFSPSAQQLLPCLGQTLYQHVMFVFPVTLLHWARSPALLPHEAPELLLLLHHILFCLLLFDMEFF
VWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELFSLGFFDMMNVTLLGCHPLTTLTFHVVNIWL
SVEDHSGYNFPWSTHRLVPFGWYGGVVHHDLHHSHFNCNFAPYFTHWDKILGTLRTASVPAR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003956
ORF Size 816 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003956.4
RefSeq Size 1378 bp
RefSeq ORF 819 bp
Locus ID 9023
UniProt ID O95992
Cytogenetics 10q23.31
Protein Families Transmembrane
Protein Pathways Primary bile acid biosynthesis
MW 31.7 kDa
Gene Summary This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. [provided by RefSeq, Jul 2008]

Other Versions

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.