MED28 (NM_025205) Human Tagged ORF Clone

CAT#: RC204304

MED28 (Myc-DDK-tagged)-Human mediator complex subunit 28 (MED28)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MED28 (Cat# RC204304, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MED28 (Cat# TA810134)(1:2000). Positive lysates LY410842 (100ug) and LC410842 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY410842) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204304 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MED28
• Synonyms: 1500003D12Rik; EG1; magicin
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204304 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTCCACTAGGGGGTATGTTTTCTGGGCAGCCACCCGGTCCCCCTCAGGCCCCGCCGGGCCTTC
  CGGGCCAAGCTTCGCTTCTTCAGGCAGCTCCAGGCGCTCCTAGACCTTCCAGCAGTACTTTGGTGGACGA
  GTTGGAGTCATCTTTCGAGGCTTGCTTTGCATCTCTGGTGAGTCAGGACTATGTCAATGGCACCGATCAG
  GAAGAAATTCGAACCGGTGTTGATCAGTGTATCCAGAAGTTTCTGGATATTGCAAGACAGACAGAATGTT
  TTTTCTTACAAAAAAGATTGCAGTTATCTGTCCAGAAACCAGAGCAAGTTATCAAAGAGGATGTGTCAGA
  ACTAAGGAATGAATTACAGCGGAAAGATGCACTAGTCCAGAAGCACTTGACAAAGCTGAGGCATTGGCAG
  CAGGTGCTGGAGGACATCAACGTGCAGCACAAAAAGCCCGCCGACATCCCTCAGGGCTCCTTGGCCTACC
  TGGAGCAGGCATCTGCCAACATCCCTGCACCTCTGAAGCCAACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204304 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAPLGGMFSGQPPGPPQAPPGLPGQASLLQAAPGAPRPSSSTLVDELESSFEACFASLVSQDYVNGTDQ
  EEIRTGVDQCIQKFLDIARQTECFFLQKRLQLSVQKPEQVIKEDVSELRNELQRKDALVQKHLTKLRHWQ
  QVLEDINVQHKKPADIPQGSLAYLEQASANIPAPLKPT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025205
• ORF Size: 534 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025205.5
• RefSeq Size: 1363 bp
• RefSeq ORF: 537 bp
• Locus ID: 80306
• UniProt ID: Q9H204
• Cytogenetics: 4p15.32
• Protein Families: Transcription Factors
• MW: 19.5 kDa
• Gene Summary: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.[UniProtKB/Swiss-Prot Function]