gamma Catenin (JUP) (NM_002230) Human Tagged ORF Clone

CAT#: RC204209

JUP (Myc-DDK-tagged)-Human junction plakoglobin (JUP), transcript variant 1

Product Images

Circular map for RC204209

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  Western blot validation of overexpression lysate (Cat# LY400807) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204209 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified JUP protein (Cat# TP304209). The protein was produced from HEK293T cells transfected with JUP cDNA clone (Cat# RC204209) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: gamma Catenin
• Synonyms: CTNNG; DP3; DPIII; PDGB; PG; PKGB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204209 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTGATGAACCTGATGGAGCAGCCTATCAAGGTGACTGAGTGGCAGCAGACATACACCTACGACT
  CGGGTATCCACTCGGGCGCCAACACCTGCGTGCCCTCCGTCAGCAGCAAGGGCATCATGGAGGAGGATGA
  GGCCTGCGGGCGCCAGTACACGCTCAAGAAAACCACCACTTACACCCAGGGGGTGCCCCCCAGCCAAGGT
  GACCTGGAGTACCAGATGTCCACAACAGCCAGGGCCAAACGGGTGCGGGAGGCCATGTGCCCTGGTGTGT
  CAGGCGAGGACAGCTCGCTTCTGCTGGCCACCCAGGTGGAGGGGCAGGCCACCAACCTGCAGCGACTGGC
  CGAGCCGTCCCAGCTGCTCAAGTCGGCCATTGTGCATCTCATCAACTACCAGGATGATGCCGAGCTGGCC
  ACTCGCGCCCTGCCCGAGCTCACCAAACTGCTCAACGACGAGGACCCGGTGGTGGTGACCAAGGCGGCCA
  TGATTGTGAACCAGCTGTCGAAGAAGGAGGCGTCGCGGCGGGCCCTGATGGGCTCGCCCCAGCTGGTGGC
  CGCTGTCGTGCGTACCATGCAGAATACCAGCGACCTGGACACAGCCCGCTGCACCACCAGCATCCTGCAC
  AACCTCTCCCACCACCGGGAGGGGCTGCTCGCCATCTTCAAGTCGGGTGGCATCCCTGCTCTGGTCCGCA
  TGCTCAGCTCCCCTGTGGAGTCGGTCCTGTTCTATGCCATCACCACGCTGCACAACCTGCTCCTGTACCA
  GGAGGGCGCCAAGATGGCCGTGCGCCTGGCCGACGGGCTGCAAAAGATGGTGCCCCTGCTCAACAAGAAC
  AACCCCAAGTTCCTGGCCATCACCACCGACTGCCTGCAGCTCCTGGCCTACGGCAACCAGGAGAGCAAGC
  TGATCATCCTGGCCAATGGTGGGCCCCAGGCCCTCGTGCAGATCATGCGTAACTACAGTTATGAAAAGCT
  GCTCTGGACCACCAGTCGTGTGCTCAAGGTGCTATCCGTGTGTCCCAGCAATAAGCCTGCCATTGTGGAG
  GCTGGTGGGATGCAGGCCCTGGGCAAGCACCTGACCAGCAACAGCCCCCGCCTGGTGCAGAACTGCCTGT
  GGACCCTGCGCAACCTCTCAGATGTGGCCACCAAGCAGGAGGGCCTGGAGAGTGTGCTGAAGATTCTGGT
  GAATCAGCTGAGTGTGGATGACGTCAACGTCCTCACCTGTGCCACGGGCACACTCTCCAACCTGACATGC
  AACAACAGCAAGAACAAGACGCTGGTGACACAGAACAGCGGTGTGGAGGCTCTCATCCATGCCATCCTGC
  GTGCTGGTGACAAGGACGACATCACGGAGCCTGCCGTCTGCGCTCTGCGCCACCTCACTAGCCGCCACCC
  TGAGGCCGAGATGGCCCAGAACTCTGTGCGTCTCAACTATGGCATCCCAGCCATCGTGAAGCTGCTCAAC
  CAGCCCAACCAGTGGCCACTGGTCAAGGCAACCATCGGCTTGATCAGGAATCTGGCCCTGTGCCCAGCCA
  ACCATGCCCCGCTGCAGGAGGCAGCGGTCATCCCCCGCCTCGTCCAACTGCTGGTGAAGGCCCACCAGGA
  TGCCCAGCGCCACGTAGCTGCAGGCACACAGCAGCCCTACACGGATGGTGTGAGGATGGAGGAGATTGTG
  GAGGGCTGCACCGGAGCACTGCACATCCTCGCCCGGGACCCCATGAACCGCATGGAGATCTTCCGGCTCA
  ACACCATTCCCCTGTTTGTGCAGCTCCTGTACTCGTCGGTGGAGAACATCCAGCGCGTGGCTGCCGGGGT
  GCTGTGTGAGCTGGCCCAGGACAAGGAGGCGGCCGACGCCATTGATGCAGAGGGGGCCTCGGCCCCACTC
  ATGGAGTTGCTGCACTCCCGCAACGAGGGCACTGCCACCTACGCTGCTGCCGTCCTGTTCCGCATCTCCG
  AGGACAAGAACCCAGACTACCGGAAGCGCGTGTCCGTGGAGCTCACCAACTCCCTCTTCAAGCATGACCC
  GGCTGCCTGGGAGGCTGCCCAGAGCATGATTCCCATCAATGAGCCCTATGGAGATGACTTGGATGCCACC
  TACCGCCCCATGTACTCCAGCGATGTGCCCCTTGACCCGCTGGAGATGCACATGGACATGGATGGAGACT
  ACCCCATCGACACCTACAGCGACGGCCTCAGGCCCCCGTACCCCACTGCAGACCACATGCTGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204209 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEVMNLMEQPIKVTEWQQTYTYDSGIHSGANTCVPSVSSKGIMEEDEACGRQYTLKKTTTYTQGVPPSQG
  DLEYQMSTTARAKRVREAMCPGVSGEDSSLLLATQVEGQATNLQRLAEPSQLLKSAIVHLINYQDDAELA
  TRALPELTKLLNDEDPVVVTKAAMIVNQLSKKEASRRALMGSPQLVAAVVRTMQNTSDLDTARCTTSILH
  NLSHHREGLLAIFKSGGIPALVRMLSSPVESVLFYAITTLHNLLLYQEGAKMAVRLADGLQKMVPLLNKN
  NPKFLAITTDCLQLLAYGNQESKLIILANGGPQALVQIMRNYSYEKLLWTTSRVLKVLSVCPSNKPAIVE
  AGGMQALGKHLTSNSPRLVQNCLWTLRNLSDVATKQEGLESVLKILVNQLSVDDVNVLTCATGTLSNLTC
  NNSKNKTLVTQNSGVEALIHAILRAGDKDDITEPAVCALRHLTSRHPEAEMAQNSVRLNYGIPAIVKLLN
  QPNQWPLVKATIGLIRNLALCPANHAPLQEAAVIPRLVQLLVKAHQDAQRHVAAGTQQPYTDGVRMEEIV
  EGCTGALHILARDPMNRMEIFRLNTIPLFVQLLYSSVENIQRVAAGVLCELAQDKEAADAIDAEGASAPL
  MELLHSRNEGTATYAAAVLFRISEDKNPDYRKRVSVELTNSLFKHDPAAWEAAQSMIPINEPYGDDLDAT
  YRPMYSSDVPLDPLEMHMDMDGDYPIDTYSDGLRPPYPTADHMLA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002230
• ORF Size: 2235 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002230.4
• RefSeq Size: 3508 bp
• RefSeq ORF: 2238 bp
• Locus ID: 3728
• UniProt ID: P14923
• Cytogenetics: 17q21.2
• Domains: Armadillo_seg
• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Pathways in cancer
• MW: 81.7 kDa
• Gene Summary: This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described. [provided by RefSeq, Jul 2008]