PARS2 (NM_152268) Human Tagged ORF Clone

CAT#: RC203961

PARS2 (Myc-DDK-tagged)-Human prolyl-tRNA synthetase 2, mitochondrial (putative) (PARS2), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC203961

• 

  Western blot validation of overexpression lysate (Cat# LY407690) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203961 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PARS2
• Synonyms: DEE75; EIEE75; MT-PRORS; proRS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203961 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGGCTGCTGACAAGATGCAGAGCATTGCCCGCCCTGGCCACCTGCAGCCGCCAGCTCTCTGGGT
  ATGTTCCTTGCAGGTTTCACCACTGTGCCCCAAGAAGAGGGCGGCGCCTGCTGCTGTCTCGTGTGTTCCA
  GCCACAGAACCTTCGGGAAGACCGGGTGCTCTCCCTGCAGGACAAATCTGATGACCTGACCTGTAAGAGC
  CAGCGGCTGATGCTGCAGGTGGGCCTGATCTACCCAGCAAGCCCCGGCTGTTACCACCTCCTGCCATATA
  CCGTCCGTGCCATGGAGAAGCTCGTGCGAGTGATAGACCAGGAGATGCAGGCCATCGGGGGCCAGAAAGT
  CAACATGCCCAGCCTCAGCCCGGCAGAGCTCTGGCAAGCCACCAACCGGTGGGACTTGATGGGCAAAGAG
  CTGCTAAGACTTAGAGACAGGCATGGCAAGGAATACTGCTTAGGACCAACTCACGAGGAAGCCATTACGG
  CCTTAATTGCCTCCCAGAAGAAACTGTCCTACAAGCAGCTTCCCTTCCTGCTGTACCAAGTGACAAGGAA
  GTTTCGGGATGAGCCCAGGCCCCGCTTTGGTCTTCTCCGTGGCCGAGAGTTTTACATGAAGGATATGTAC
  ACCTTTGACTCCTCCCCAGAGGCTGCCCAGCAGACCTACAGCCTGGTGTGTGATGCCTACTGCAGCCTGT
  TCAACAAGCTAGGGCTGCCATTTGTCAAGGTCCAGGCCGATGTGGGCACCATCGGGGGCACAGTGTCTCA
  TGAGTTCCAGCTCCCAGTGGATATTGGAGAGGACCGGCTTGCCATCTGTCCCCGCTGCAGCTTCTCAGCC
  AACATGGAGACACTAGACTTGTCACAAATGAACTGCCCTGCTTGCCAGGGCCCATTGACTAAAACCAAAG
  GCATTGAGGTGGGGCACACATTTTACCTGGGTACCAAGTACTCATCCATTTTCAATGCCCAGTTTACCAA
  TGTCTGTGGCAAACCAACCCTGGCTGAAATGGGGTGCTATGGCTTGGGTGTGACACGGATCTTGGCTGCT
  GCCATTGAAGTCCTCTCTACAGAAGACTGTGTCCGCTGGCCCAGCCTACTGGCCCCTTACCAAGCCTGCC
  TCATCCCCCCTAAGAAGGGCAGTAAGGAGCAGGCGGCCTCCGAGCTCATAGGGCAGCTGTACGACCACAT
  CACAGAGGCAGTGCCTCAGCTTCACGGGGAGGTGCTCCTGGACGACAGGACCCATCTGACCATCGGAAAC
  AGACTGAAAGATGCCAACAAGTTTGGCTACCCCTTTGTGATAATCGCTGGCAAGAGGGCCCTGGAGGACC
  CTGCACATTTTGAGGTTTGGTGTCAGAACACTGGTGAGGTGGCCTTCCTCACCAAAGATGGAGTCATGGA
  TTTACTGACCCCAGTGCAGACTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203961 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEGLLTRCRALPALATCSRQLSGYVPCRFHHCAPRRGRRLLLSRVFQPQNLREDRVLSLQDKSDDLTCKS
  QRLMLQVGLIYPASPGCYHLLPYTVRAMEKLVRVIDQEMQAIGGQKVNMPSLSPAELWQATNRWDLMGKE
  LLRLRDRHGKEYCLGPTHEEAITALIASQKKLSYKQLPFLLYQVTRKFRDEPRPRFGLLRGREFYMKDMY
  TFDSSPEAAQQTYSLVCDAYCSLFNKLGLPFVKVQADVGTIGGTVSHEFQLPVDIGEDRLAICPRCSFSA
  NMETLDLSQMNCPACQGPLTKTKGIEVGHTFYLGTKYSSIFNAQFTNVCGKPTLAEMGCYGLGVTRILAA
  AIEVLSTEDCVRWPSLLAPYQACLIPPKKGSKEQAASELIGQLYDHITEAVPQLHGEVLLDDRTHLTIGN
  RLKDANKFGYPFVIIAGKRALEDPAHFEVWCQNTGEVAFLTKDGVMDLLTPVQTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152268
• ORF Size: 1425 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152268.4
• RefSeq Size: 2403 bp
• RefSeq ORF: 1428 bp
• Locus ID: 25973
• UniProt ID: Q7L3T8
• Cytogenetics: 1p32.3
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• MW: 53.3 kDa
• Gene Summary: This gene encodes a putative member of the class II family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of proline to tRNA molecules. Mutations have been found in this gene in some patients with Alpers syndrome. [provided by RefSeq, Mar 2015]