PARS2 (NM_152268) Human Untagged Clone

CAT#: SC123237

PARS2 (untagged)-Human prolyl-tRNA synthetase 2, mitochondrial (putative) (PARS2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PARS2
• Synonyms: DEE75; EIEE75; MT-PRORS; proRS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_152268 edited
  GCGCGCTGGCCCGGCACGGCGGTGGTCTTGCGGGAGGCGTGGGCTGGGATTGCGGTGCCT
  GTGCTTCCCGGTGCCAGGGTGTCATGGAAGGGCTGCTGACAAGATGCAGAGCATTGCCCG
  CCCTGGCCACCTGCAGCCGCCAGCTCTCTGGGTATGTTCCTTGCAGGTTTCACCACTGTG
  CCCCAAGAAGAGGGCGGCGCCTGCTGCTGTCTCGTGTGTTCCAGCCACAGAACCTTCGGG
  AAGACCGGGTGCTCTCCCTGCAGGACAAATCTGATGACCTGACCTGTAAGAGCCAGCGGC
  TGATGCTGCAGGTGGGCCTGATCTACCCAGCAAGCCCCGGCTGTTACCACCTCCTGCCAT
  ATACCGTCCGTGCCATGGAGAAGCTCGTGCGAGTGATAGACCAGGAGATGCAGGCCATCG
  GGGGCCAGAAAGTCAACATGCCCAGCCTCAGCCCGGCAGAGCTCTGGCAAGCCACCAACC
  GGTGGGACTTGATGGGCAAAGAGCTGCTAAGACTTAGAGACAGGCATGGCAAGGAATACT
  GCTTAGGACCAACTCACGAGGAAGCCATTACGGCCTTAATTGCCTCCCAGAAGAAACTGT
  CCTACAAGCAGCTTCCCTTCCTGCTGTACCAAGTGACAAGGAAGTTTCGGGATGAGCCCA
  GGCCCCGCTTTGGTCTTCTCCGTGGCCGAGAGTTTTACATGAAGGATATGTACACCTTTG
  ACTCCTCCCCAGAGGCTGCCCAGCAGACCTACAGCCTGGTGTGTGATGCCTACTGCAGCC
  TGTTCAACAAGCTAGGGCTGCCATTTGTCAAGGTCCAGGCCGATGTGGGCACCATCGGGG
  GCACAGTGTCTCATGAGTTCCAGCTCCCAGTGGATATTGGAGAGGACCGGCTTGCCATCT
  GTCCCCGCTGCAGCTTCTCAGCCAACATGGAGACACTAGACTTGTCACAAATGAACTGCC
  CTGCTTGCCAGGGCCCATTGACTAAAACCAAAGGCATTGAGGTGGGGCACACATTTTACC
  TGGGTACCAAGTACTCATCCATTTTCAATGCCCAGTTTACCAATGTCTGTGGCAAACCAA
  CCCTGGCTGAAATGGGGTGCTATGGCTTGGGTGTGACACGGATCTTGGCTGCTGCCATTG
  AAGTCCTCTCTACAGAAGACTGTGTCCGCTGGCCCAGCCTACTGGCCCCTTACCAAGCCT
  GCCTCATCCCCCCTAAGAAGGGCAGTAAGGAGCAGGCGGCCTCCGAGCTCATAGGGCAGC
  TGTACGACCACATCACAGAGGCAGTGCCTCAGCTTCACGGGGAGGTGCTCCTGGACGACA
  GGACCCATCTGACCATCGGAAACAGACTGAAAGATGCCAACAAGTTTGGCTACCCCTTTG
  TGATAATCGCTGGCAAGAGGGCCCTGGAGGACCCTGCACATTTTGAGGTTTGGTGTCAGA
  ACACTGGTGAGGTGGCCTTCCTCACCAAAGATGGAGTCATGGATTTACTGACCCCAGTGC
  AGACTGTCTAAATGCCCCCAGCCCACCCCTGCCCCCATTTGCAGCCTTGGTGTTCGTTCT
  AACACTGCATTTTCCTACACCCCTTTCCTGGACTGCTCTCTCCAGAAACAGCACAGCTCA
  TGGAGGGTGAGATCATGTTAGGGAAATCAATTTTTATCTAGTCATTTGTTCAGATTATTT
  GTATTTAAAGTCATTAACTTGATCCCTTTCTCCAGACTGGCCATGCCGCCATATACCTTT
  CCTTTTGTATTCCATTGTGGAAGCTTCTAGGAGGCTGAGAAGCAGGAGATCCAAGCTCCA
  GTCCTGGGTGTTACCCTGAGGGGGGAAGTCACTTCCCTTCTCTCAGCTGAGTTTTTCACG
  TGTAAGATGAGAATGGCTGATGGCTGAGAGCCCACCAACCTTCCCCACCCCGTATCAGTT
  TGACCACGGGCCTTTTGCTACATTCTATCCAGAGAGCAGGCCTGCCAGCCCCTCTCCACT
  GAGAAGTTGGGCTGAGAGTATGGGGAAAAGAATCAAGAGACCTGACTGCCGCCAACTCAC
  TAGTGACTTAGATACATCCCCTCCCCCTGCTGGGGCCTCAGTTTCCCCATCTGTAACTTG
  AGAAAATAGAACTAGATCTGTAAAGTCTGGAACTAGATCTGTAACGTCCTTATAGGTGTC
  ACTAGGGGGTTCCATGAGAGGTGTGTGACAGGCAGTCTGATTCCTCTCATTCTCCATAGT
  CTGTTTCCTGGAAAGTCGATGTAATTAACTGATGGCCCAAAAACTACCTCAAGAGACCTG
  GCCCTGTTAAGACGGCTTAACCACTGAGATCCCGTTCTATTGATTTAATAAAGTCAAACT
  ATTGGAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152268 unedited
  TAGATTTGTATACGACTTATATAGGCGGCCGACGAATTCGCACGAGGGCGCGCTGGCCCG
  GCACGGCGGTGGTCTTGCGGGAGGCGTGGGCTGGGATTGCGGTGCCTGTGCTTCCCGGTG
  CCAGGGTGTCATGGAAGGGCTGCTGACAAGATGCAGAGCATTGCCCGCCCTGGCCACCTG
  CAGCCGCCAGCTCTCTGGGTATGTTCCTTGCAGGTTTCACCACTGTGCCCCAAGAAGAGG
  GCGGCGCCTGCTGCTGTCTCGTGTGTTCCAGCCACAGAACCTTCGGGAAGACCGGGTGCT
  CTCCCTGCAGGACAAATCTGATGACCTGACCTGTAAGAGCCAGCGGCTGATGCTGCAGGT
  GGGCCTGATCTACCCAGCAAGCCCCGGCTGTTACCACCTCCTGCCATATACCGTCCGTGC
  CATGGAGAAGCTCGTGCGAGTGATAGACCAGGAGATGCAGGCCATCGGGGGCCAGATAGT
  CAACATGCCCAGCCTCAGCCCGGCAGAGCTCTGGCAAGCCACCAACCGGTGGGACTTGAT
  GGGCAAAGAGCTGCTAAGACTTATAGACAGGCATGGCAAGGAATACTGCTTAGGACCAAC
  TCACGAGGAAGCCCTTACGGCCTTAATTGCCTCCCAGAAGATACTGTCCTACAAGCAGCT
  TTCCCTTCCTGCTGTACCAAGTGACAAAGAAGTTTCCGGGTGAGCCCAAGCCCCCGCTTT
  GGTCTTTCTCGTGGCCGAGAGTTTTACTGAAGGATATGTACACCTTTGACTCCTCCCCAA
  AAGCTGCCCAACAAAACTACAGCCTGGTGTGGGATGCCTACTGCAGCCTGGTCAACAAGC
  TAGGGCTGCCATTTGTCAAGGTCCAGCCCGATTTGGCCCCCTCCGGAGCCCAATGGTCTA
  ATAATTCCAACTCCAATGGAATTTTGGAAAGGACCGGTTGCCAAACTTC
  
• Restriction Sites: Please inquire
• ACCN: NM_152268
• Insert Size: 2373 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152268.2, NP_689481.2
• RefSeq Size: 2364 bp
• RefSeq ORF: 1428 bp
• Locus ID: 25973
• UniProt ID: Q7L3T8
• Cytogenetics: 1p32.3
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• Gene Summary: This gene encodes a putative member of the class II family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of proline to tRNA molecules. Mutations have been found in this gene in some patients with Alpers syndrome. [provided by RefSeq, Mar 2015]