EFEMP2 (NM_016938) Human Tagged ORF Clone

CAT#: RC203726

EFEMP2 (Myc-DDK-tagged)-Human EGF containing fibulin-like extracellular matrix protein 2 (EFEMP2), transcript variant 1

Product Images

Circular map for RC203726

• 

  Western blot validation of overexpression lysate (Cat# LY402585) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203726 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EFEMP2
• Synonyms: ARCL1B; FBLN4; MBP1; UPH1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203726 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCCCTGCGCCTCCTGCCTACCCGGGTCTCTACTGCTCTGGGCGCTGCTACTGTTGCTCTTGGGAT
  CAGCTTCTCCTCAGGATTCTGAAGAGCCCGACAGCTACACGGAATGCACAGATGGCTATGAGTGGGACCC
  AGACAGCCAGCACTGCCGGGATGTCAACGAGTGTCTGACCATCCCTGAGGCCTGCAAGGGGGAAATGAAG
  TGCATCAACCACTACGGGGGCTACTTGTGCCTGCCCCGCTCCGCTGCCGTCATCAACGACCTACACGGCG
  AGGGACCCCCGCCACCAGTGCCTCCCGCTCAACACCCCAACCCCTGCCCACCAGGCTATGAGCCCGACGA
  TCAGGACAGCTGTGTGGATGTGGACGAGTGTGCCCAGGCCCTGCACGACTGTCGCCCCAGCCAGGACTGC
  CATAACTTGCCTGGCTCCTATCAGTGCACCTGCCCTGATGGTTACCGCAAGATCGGGCCCGAGTGTGTGG
  ACATAGACGAGTGCCGCTACCGCTACTGCCAGCACCGCTGCGTGAACCTGCCTGGCTCCTTCCGCTGCCA
  GTGCGAGCCGGGCTTCCAGCTGGGGCCTAACAACCGCTCCTGTGTTGATGTGAACGAGTGTGACATGGGG
  GCCCCATGCGAGCAGCGCTGCTTCAACTCCTATGGGACCTTCCTGTGTCGCTGCCACCAGGGCTATGAGC
  TGCATCGGGATGGCTTCTCCTGCAGTGATATTGATGAGTGTAGCTACTCCAGCTACCTCTGTCAGTACCG
  CTGCGTCAACGAGCCAGGCCGTTTCTCCTGCCACTGCCCACAGGGTTACCAGCTGCTGGCCACACGCCTC
  TGCCAAGACATTGATGAGTGTGAGTCTGGTGCGCACCAGTGCTCCGAGGCCCAAACCTGTGTCAACTTCC
  ATGGGGGCTACCGCTGCGTGGACACCAACCGCTGCGTGGAGCCCTACATCCAGGTCTCTGAGAACCGCTG
  TCTCTGCCCGGCCTCCAACCCTCTATGTCGAGAGCAGCCTTCATCCATTGTGCACCGCTACATGACCATC
  ACCTCGGAGCGGAGCGTGCCCGCTGACGTGTTCCAGATCCAGGCGACCTCCGTCTACCCCGGTGCCTACA
  ATGCCTTTCAGATCCGTGCTGGAAACTCGCAGGGGGACTTTTACATTAGGCAAATCAACAACGTCAGCGC
  CATGCTGGTCCTCGCCCGGCCGGTGACGGGCCCCCGGGAGTACGTGCTGGACCTGGAGATGGTCACCATG
  AATTCCCTCATGAGCTACCGGGCCAGCTCTGTACTGAGGCTCACCGTCTTTGTAGGGGCCTACACCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203726 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLPCASCLPGSLLLWALLLLLLGSASPQDSEEPDSYTECTDGYEWDPDSQHCRDVNECLTIPEACKGEMK
  CINHYGGYLCLPRSAAVINDLHGEGPPPPVPPAQHPNPCPPGYEPDDQDSCVDVDECAQALHDCRPSQDC
  HNLPGSYQCTCPDGYRKIGPECVDIDECRYRYCQHRCVNLPGSFRCQCEPGFQLGPNNRSCVDVNECDMG
  APCEQRCFNSYGTFLCRCHQGYELHRDGFSCSDIDECSYSSYLCQYRCVNEPGRFSCHCPQGYQLLATRL
  CQDIDECESGAHQCSEAQTCVNFHGGYRCVDTNRCVEPYIQVSENRCLCPASNPLCREQPSSIVHRYMTI
  TSERSVPADVFQIQATSVYPGAYNAFQIRAGNSQGDFYIRQINNVSAMLVLARPVTGPREYVLDLEMVTM
  NSLMSYRASSVLRLTVFVGAYTF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016938
• ORF Size: 1329 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016938.3
• RefSeq Size: 2096 bp
• RefSeq ORF: 1332 bp
• Locus ID: 30008
• UniProt ID: O95967
• Cytogenetics: 11q13.1
• Domains: EGF_CA, EGF, EGF
• Protein Families: Druggable Genome, Secreted Protein
• MW: 49.4 kDa
• Gene Summary: A large number of extracellular matrix proteins have been found to contain variations of the epidermal growth factor (EGF) domain and have been implicated in functions as diverse as blood coagulation, activation of complement and determination of cell fate during development. The protein encoded by this gene contains four EGF2 domains and six calcium-binding EGF2 domains. This gene is necessary for elastic fiber formation and connective tissue development. Defects in this gene are cause of an autosomal recessive cutis laxa syndrome. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jan 2011]