Neugrin (NGRN) (NM_016645) Human Tagged ORF Clone

CAT#: RC203415

NGRN (Myc-DDK-tagged)-Human neugrin, neurite outgrowth associated (NGRN), transcript variant 1

Product Images

Circular map for RC203415

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  Western blot validation of overexpression lysate (Cat# LY413846) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203415 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NGRN protein (Cat# TP303415). The protein was produced from HEK293T cells transfected with NGRN cDNA clone (Cat# RC203415) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Neugrin
• Synonyms: DSC92; mesenchymal stem cell protein DSC92; neugrin, neurite outgrowth associated; neurite outgrowth associated protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203415 representing NM_016645
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGCCTGGTGCCCCGCCCAGGACCCTGACGTGGGAAGCCATGGAGCAGATACGGTATTTACATG
  AGGAATTTCCAGAGTCCTGGTCAGTTCCCAGGTTGGCTGAAGGCTTTGATGTCAGCACTGATGTGATCCG
  AAGAGTTTTAAAAAGCAAGTTTTTACCCACATTGGAGCAGAAGCTGAAGCAGGATCAAAAAGTCCTTAAG
  AAAGCTGGGCTTGCCCACTCGCTGCAGCACCTCCGGGGCTCTGGAAATACCTCAAAGCTGCTCCCTGCAG
  GCCACTCTGTATCAGGCTCTTTGCTTATGCCAGGGCATGAAGCCTCATCTAAAGACCCAAATCACAGCAC
  AGCTTTGAAAGTGATAGAGTCAGACACTCACAGGACAAATACACCAAGGAGAAGGAAGGGAAGAAATAAA
  GAAATCCAGGACCTGGAGGAGAGCTTTGTGCCTGTTGCTGCACCCCTAGGTCATCCAAGAGAGCTGCAGA
  AGTACTCCAGTGATTCTGAGAGCCCCAGAGGAACTGGCAGTGGTGCGTTGCCAAGTGGTCAGAAGCTGGA
  GGAGTTGAAGGCAGAGGAGCCAGATAACTTCAGCAGCAAAGTAGTGCAGAGGGGCCGAGAGTTCTTTGAC
  AGCAACGGGAACTTCCTGTACAGAATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203415 representing NM_016645
  Red=Cloning site Green=Tags(s)
  
  MEAPGAPPRTLTWEAMEQIRYLHEEFPESWSVPRLAEGFDVSTDVIRRVLKSKFLPTLEQKLKQDQKVLK
  KAGLAHSLQHLRGSGNTSKLLPAGHSVSGSLLMPGHEASSKDPNHSTALKVIESDTHRTNTPRRRKGRNK
  EIQDLEESFVPVAAPLGHPRELQKYSSDSESPRGTGSGALPSGQKLEELKAEEPDNFSSKVVQRGREFFD
  SNGNFLYRI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016645
• ORF Size: 657 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016645.2, NP_057729.1
• RefSeq Size: 1782 bp
• RefSeq ORF: 659 bp
• Locus ID: 51335
• Cytogenetics: 15q26.1
• MW: 24.2 kDa
• Gene Summary: Plays an essential role in mitochondrial ribosome biogenesis. As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mitochondrial ribosomal RNA (16S mt-rRNA), controls 16S mt-rRNA abundance and is required for intra-mitochondrial translation of core subunits of the oxidative phosphorylation system.[UniProtKB/Swiss-Prot Function]