RTN4IP1 (NM_032730) Human Tagged ORF Clone

CAT#: RC202957

RTN4IP1 (Myc-DDK-tagged)-Human reticulon 4 interacting protein 1 (RTN4IP1), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC202957

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RTN4IP1 (Cat# RC202957, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RTN4IP1(Cat# TA502544). Positive lysates LY409966 (100ug) and LC409966 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY409966) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202957 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RTN4IP1 protein (Cat# TP302957). The protein was produced from HEK293T cells transfected with RTN4IP1 cDNA clone (Cat# RC202957) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RTN4IP1
• Synonyms: NIMP; OPA10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202957 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAATTTCTGAAGACTTGTGTACTTAGAAGAAATGCATGCACTGCGGTTTGCTTCTGGAGAAGCAAAG
  TTGTCCAAAAGCCTTCAGTTAGAAGGATTAGTACTACCTCTCCTAGGAGCACTGTCATGCCTGCTTGGGT
  GATAGATAAATATGGGAAGAATGAAGTGCTTCGATTCACTCAGAACATGATGATGCCTATCATACACTAT
  CCAAATGAAGTCATTGTCAAAGTTCACGCTGCCAGTGTAAATCCTATAGACGTTAATATGAGAAGTGGTT
  ATGGAGCTACAGCTTTAAATATGAAGCGTGATCCTTTACACGTGAAAATCAAAGGAGAAGAATTTCCTCT
  GACTCTGGGTCGGGATGTCTCTGGCGTGGTGATGGAATGTGGGCTTGATGTGAAATACTTCAAGCCTGGA
  GATGAGGTCTGGGCTGCAGTTCCTCCTTGGAAACAAGGCACTCTTTCAGAGTTTGTTGTAGTCAGTGGGA
  ATGAGGTCTCTCACAAACCCAAATCACTCACTCATACTCAAGCTGCCTCTTTGCCATATGTGGCTCTCAC
  AGCCTGGTCTGCTATAAACAAAGTTGGTGGCCTGAATGACAAGAATTGCACAGGAAAACGTGTTCTAATC
  TTAGGCGCTTCAGGCGGAGTTGGTACTTTTGCTATACAGGTAATGAAAGCATGGGATGCTCATGTGACAG
  CAGTTTGCTCCCAAGATGCCAGTGAACTTGTAAGGAAGCTTGGTGCAGACGATGTAATTGATTACAAATC
  TGGAAGTGTGGAAGAGCAGTTGAAATCCTTAAAACCATTTGATTTTATCCTTGATAATGTTGGCGGATCC
  ACTGAAACATGGGCTCCAGATTTTCTCAAGAAATGGTCAGGAGCCACCTATGTGACTTTGGTGACTCCTT
  TCCTCCTGAACATGGACCGATTGGGCATAGCAGATGGCATGTTGCAGACAGGAGTCACTGTAGGTTCAAA
  GGCATTAAAGCATTTCTGGAAAGGAGTCCATTATCGCTGGGCATTTTTCATGGCCAGTGGCCCATGTTTA
  GATGACATTGCAGAACTGGTGGATGCGGGAAAGATCCGGCCAGTTATTGAACAAACCTTTCCTTTTTCTA
  AAGTTCCAGAAGCCTTCCTGAAGGTGGAAAGAGGACACGCACGAGGAAAGACTGTAATTAATGTTGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202957 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEFLKTCVLRRNACTAVCFWRSKVVQKPSVRRISTTSPRSTVMPAWVIDKYGKNEVLRFTQNMMMPIIHY
  PNEVIVKVHAASVNPIDVNMRSGYGATALNMKRDPLHVKIKGEEFPLTLGRDVSGVVMECGLDVKYFKPG
  DEVWAAVPPWKQGTLSEFVVVSGNEVSHKPKSLTHTQAASLPYVALTAWSAINKVGGLNDKNCTGKRVLI
  LGASGGVGTFAIQVMKAWDAHVTAVCSQDASELVRKLGADDVIDYKSGSVEEQLKSLKPFDFILDNVGGS
  TETWAPDFLKKWSGATYVTLVTPFLLNMDRLGIADGMLQTGVTVGSKALKHFWKGVHYRWAFFMASGPCL
  DDIAELVDAGKIRPVIEQTFPFSKVPEAFLKVERGHARGKTVINVV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032730
• ORF Size: 1188 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032730.5
• RefSeq Size: 2636 bp
• RefSeq ORF: 1191 bp
• Locus ID: 84816
• UniProt ID: Q8WWV3
• Cytogenetics: 6q21
• Domains: ADH_zinc_N
• Protein Families: Druggable Genome
• MW: 43.6 kDa
• Gene Summary: This gene encodes a mitochondrial protein that interacts with reticulon 4, which is a potent inhibitor of regeneration following spinal cord injury. This interaction may be important for reticulon-induced inhibition of neurite growth. Mutations in this gene can cause optic atrophy 10, with or without ataxia, cognitive disability, and seizures. There is a pseudogene for this gene on chromosome 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]