RTN4IP1 (NM_032730) Human Untagged Clone

CAT#: SC320174

RTN4IP1 (untagged)-Human reticulon 4 interacting protein 1 (RTN4IP1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RTN4IP1
• Synonyms: NIMP; OPA10
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_032730.4 AAGTGGAGGGGGTGAAAGTGAGGGAGGAGAATGGACAGAATACTGACTGGAACGTTAATT
  CGAGCATTTCATATGCGAAGAGCGGAATAACAGTTCCGTATTCTTCTTTCAGTTTCTCCA
  TTAGATTAGCTTCATTTTCGAATGCTCCGTTTTGCATGCTTAATTTTGAAACTAGCCCGT
  GGTTTGGCAGAATTTGACTGAATTCAGGGGTGAGAGTTTGATCCAGTCCAAGTGTATTTG
  AATTTGAGCACGCAGTTCAACCAGTGTTTACAATGGAATTTCTGAAGACTTGTGTACTTA
  GAAGAAATGCATGCACTGCGGTTTGCTTCTGGAGAAGCAAAGTTGTCCAAAAGCCTTCAG
  TTAGAAGGATTAGTACTACCTCTCCTAGGAGCACTGTCATGCCTGCTTGGGTGATAGATA
  AATATGGGAAGAATGAAGTGCTTCGATTCACTCAGAACATGATGATGCCTATCATACACT
  ATCCAAATGAAGTCATTGTCAAAGTTCACGCTGCCAGTGTAAATCCTATAGACGTTAATA
  TGAGAAGTGGTTATGGAGCTACAGCTTTAAATATGAAGCGTGATCCTTTACACGTGAAAA
  TCAAAGGAGAAGAATTTCCTCTGACTCTGGGTCGGGATGTCTCTGGCGTGGTGATGGAAT
  GTGGGCTTGATGTGAAATACTTCAAGCCTGGAGATGAGGTCTGGGCTGCAGTTCCTCCTT
  GGAAACAAGGCACTCTTTCAGAGTTTGTTGTAGTCAGTGGGAATGAGGTCTCTCACAAAC
  CCAAATCACTCACTCATACTCAAGCTGCCTCTTTGCCATATGTGGCTCTCACAGCCTGGT
  CTGCTATAAACAAAGTTGGTGGCCTGAATGACAAGAATTGCACAGGAAAACGTGTTCTAA
  TCTTAGGCGCTTCAGGCGGAGTTGGTACTTTTGCTATACAGGTAATGAAAGCATGGGATG
  CTCATGTGACAGCAGTTTGCTCCCAAGATGCCAGTGAACTTGTAAGGAAGCTTGGTGCAG
  ACGATGTAATTGATTACAAATCTGGAAGTGTGGAAGAGCAGTTGAAATCCTTAAAACCAT
  TTGATTTTATCCTTGATAATGTTGGCGGATCCACTGAAACATGGGCTCCAGATTTTCTCA
  AGAAATGGTCAGGAGCCACCTATGTGACTTTGGTGACTCCTTTCCTCCTGAACATGGACC
  GATTGGGCATAGCAGATGGCATGTTGCAGACAGGAGTCACTGTAGGTTCAAAGGCATTAA
  AGCATTTCTGGAAAGGAGTCCATTATCGCTGGGCATTTTTCATGGCCAGTGGCCCATGTT
  TAGATGACATTGCAGAACTGGTGGATGCGGGAAAGATCCGGCCAGTTATTGAACAAACCT
  TTCCTTTTTCTAAAGTTCCAGAAGCCTTCCTGAAGGTGGAAAGAGGACACGCACGAGGAA
  AGACTGTAATTAATGTTGTTTAAATAAAAATGCAGTTTAGTGAAAAAAAAAAAAAAAAAA
  AAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_032730
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032730.4, NP_116119.2
• RefSeq Size: 2636 bp
• RefSeq ORF: 1191 bp
• Locus ID: 84816
• UniProt ID: Q8WWV3
• Cytogenetics: 6q21
• Domains: ADH_zinc_N
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a mitochondrial protein that interacts with reticulon 4, which is a potent inhibitor of regeneration following spinal cord injury. This interaction may be important for reticulon-induced inhibition of neurite growth. Mutations in this gene can cause optic atrophy 10, with or without ataxia, cognitive disability, and seizures. There is a pseudogene for this gene on chromosome 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.