CRMP5 (DPYSL5) (NM_020134) Human Tagged ORF Clone

CAT#: RC202631

DPYSL5 (Myc-DDK-tagged)-Human dihydropyrimidinase-like 5 (DPYSL5)

Product Images

Circular map for RC202631

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  Western blot validation of overexpression lysate (Cat# LY412641) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202631 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DPYSL5 protein (Cat# TP302631). The protein was produced from HEK293T cells transfected with DPYSL5 cDNA clone (Cat# RC202631) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CRMP5
• Synonyms: CRAM; CRMP-5; CRMP5; CV2; Ulip6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202631 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTGCCAACTCAGCCAGCGTGAGGATCCTCATCAAGGGAGGCAAGGTGGTGAACGATGACTGCACCC
  ACGAGGCTGACGTCTACATCGAGAATGGCATCATCCAGCAGGTGGGCCGCGAGCTCATGATCCCTGGCGG
  GGCCAAGGTGATTGATGCCACAGGAAAACTGGTGATCCCTGGTGGCATCGACACCAGCACCCACTTCCAC
  CAGACCTTCATGAATGCCACGTGCGTGGACGACTTCTACCATGGGACCAAGGCAGCACTCGTCGGAGGCA
  CCACCATGATCATCGGCCACGTCCTGCCCGACAAGGAGACCTCCCTTGTGGACGCTTATGAGAAGTGCCG
  AGGTCTGGCCGACCCCAAGGTCTGCTGTGATTACGCCCTCCACGTGGGGATCACCTGGTGGGCACCCAAG
  GTGAAAGCAGAAATGGAGACACTGGTGAGGGAGAAGGGTGTCAACTCGTTCCAGATGTTCATGACCTACA
  AGGACCTGTACATGCTTCGAGACAGTGAGCTGTACCAAGTGTTGCACGCTTGCAAGGACATTGGGGCAAT
  CGCCCGCGTCCATGCTGAAAATGGGGAGCTTGTGGCCGAGGGTGCTAAGGAGGCACTGGATTTGGGGATC
  ACAGGCCCAGAAGGAATCGAGATCAGCCGTCCAGAGGAGCTGGAAGCTGAAGCCACTCATCGTGTTATCA
  CCATTGCAAACAGGACTCACTGTCCAATCTACCTGGTCAACGTGTCCAGTATCTCGGCTGGTGACGTTAT
  CGCAGCTGCTAAGATGCAAGGGAAGGTTGTGCTGGCGGAGACCACCACTGCACATGCCACGCTGACAGGC
  TTACACTACTACCACCAGGACTGGTCCCACGCGGCTGCCTATGTCACGGTGCCTCCCCTGAGACTGGACA
  CCAACACCTCAACCTACCTCATGAGCCTGCTGGCCAATGACACTCTGAACATCGTGGCATCAGATCACCG
  GCCTTTCACCACAAAGCAGAAAGCTATGGGCAAGGAAGACTTCACCAAGATCCCACATGGAGTGAGTGGC
  GTGCAGGACCGCATGAGCGTCATCTGGGAGAGAGGAGTGGTTGGAGGAAAGATGGATGAGAACCGTTTTG
  TGGCCGTTACCAGTTCCAACGCAGCTAAGCTTCTGAACCTGTATCCCCGCAAGGGCCGCATTATTCCCGG
  AGCCGATGCTGATGTGGTGGTGTGGGACCCAGAAGCCACAAAGACCATCTCAGCCAGCACGCAGGTCCAG
  GGAGGAGACTTCAACCTGTATGAGAACATGCGCTGCCACGGCGTGCCACTGGTCACCATCAGCCGGGGGC
  GCGTCGTGTATGAGAACGGCGTCTTCATGTGCGCCGAGGGCACCGGCAAGTTCTGTCCCCTGAGGTCCTT
  CCCAGACACTGTCTACAAGAAGCTGGTCCAGAGAGAGAAGACTTTAAAGGTTAGAGGAGTGGACCGCACT
  CCCTACCTGGGGGATGTCGCTGTTGTCGTGCACCCTGGGAAAAAAGAGATGGGAACCCCACTCGCAGACA
  CTCCTACCCGGCCCGTCACCCGGCATGGGGGCATGAGGGACCTTCACGAATCCAGCTTCAGCCTCTCTGG
  CTCTCAGATCGATGACCATGTTCCAAAGCGAGCTTCAGCTCGGATCCTCGCTCCTCCCGGAGGCAGGTCG
  AGTGGCATTTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202631 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLANSASVRILIKGGKVVNDDCTHEADVYIENGIIQQVGRELMIPGGAKVIDATGKLVIPGGIDTSTHFH
  QTFMNATCVDDFYHGTKAALVGGTTMIIGHVLPDKETSLVDAYEKCRGLADPKVCCDYALHVGITWWAPK
  VKAEMETLVREKGVNSFQMFMTYKDLYMLRDSELYQVLHACKDIGAIARVHAENGELVAEGAKEALDLGI
  TGPEGIEISRPEELEAEATHRVITIANRTHCPIYLVNVSSISAGDVIAAAKMQGKVVLAETTTAHATLTG
  LHYYHQDWSHAAAYVTVPPLRLDTNTSTYLMSLLANDTLNIVASDHRPFTTKQKAMGKEDFTKIPHGVSG
  VQDRMSVIWERGVVGGKMDENRFVAVTSSNAAKLLNLYPRKGRIIPGADADVVVWDPEATKTISASTQVQ
  GGDFNLYENMRCHGVPLVTISRGRVVYENGVFMCAEGTGKFCPLRSFPDTVYKKLVQREKTLKVRGVDRT
  PYLGDVAVVVHPGKKEMGTPLADTPTRPVTRHGGMRDLHESSFSLSGSQIDDHVPKRASARILAPPGGRS
  SGIW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020134
• ORF Size: 1692 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020134.4
• RefSeq Size: 5225 bp
• RefSeq ORF: 1695 bp
• Locus ID: 56896
• UniProt ID: Q9BPU6
• Cytogenetics: 2p23.3
• Domains: Amidohydro_1
• Protein Pathways: Axon guidance
• MW: 61.4 kDa
• Gene Summary: This gene encodes a member of the CRMP (collapsing response mediator protein) family thought to be involved in neural development. Antibodies to the encoded protein were found in some patients with neurologic symptoms who had paraneoplastic syndrome. A pseudogene of this gene is found on chromosome 11. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Dec 2011]