PSMD6 (NM_014814) Human Tagged ORF Clone

CAT#: RC202292

PSMD6 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 6 (PSMD6)

Product Images

Circular map for RC202292

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  Western blot validation of overexpression lysate (Cat# LY415023) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202292 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PSMD6 protein (Cat# TP302292). The protein was produced from HEK293T cells transfected with PSMD6 cDNA clone (Cat# RC202292) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PSMD6
• Synonyms: p42A; p44S10; Rpn7; S10; SGA-113M
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202292 representing NM_014814
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCTGGAGAACCTGGAGGAGGAGGGTCTGCCCAAGAACCCCGACTTGCGTATCGCGCAGCTGCGCT
  TCCTGCTCAGCCTGCCCGAGCACCGCGGAGACGCTGCCGTGCGCGACGAGCTGATGGCGGCCGTCCGCGA
  TAACAACATGGCTCCTTACTATGAAGCCTTGTGCAAATCCCTCGACTGGCAGATAGACGTGGACCTACTC
  AATAAAATGAAGAAGGCAAATGAAGATGAGTTGAAGCGTTTGGATGAGGAGCTGGAAGATGCAGAGAAGA
  ATCTAGGAGAGAGCGAAATTCGCGATGCAATGATGGCAAAGGCCGAGTACCTCTGCCGGATAGGTGACAA
  AGAGGGAGCTCTGACAGCCTTTCGCAAGACATATGACAAAACTGTGGCCCTGGGTCACCGATTGGATATT
  GTATTCTATCTCCTTAGGATTGGCTTATTTTATATGGATAATGATCTCATCACACGAAACACAGAAAAGG
  CCAAAAGCTTAATAGAAGAAGGAGGAGACTGGGACAGGAGAAACCGCCTAAAAGTGTATCAGGGTCTTTA
  TTGTGTGGCTATTCGTGATTTCAAACAGGCAGCTGAACTCTTCCTTGACACTGTTTCAACATTTACATCC
  TATGAACTCATGGATTATAAAACATTTGTGACTTATACTGTCTATGTCAGTATGATTGCCTTAGAAAGAC
  CAGATCTCAGGGAAAAGGTCATTAAAGGAGCAGAGATTCTTGAAGTGTTGCACAGTCTTCCAGCAGTTCG
  GCAGTATCTGTTTTCACTCTATGAATGCCGTTACTCTGTTTTCTTCCAATCATTAGCGGTTGTGGAACAG
  GAAATGAAAAAGGACTGGCTTTTTGCCCCTCATTATCGATACTATGTAAGAGAAATGAGAATTCATGCAT
  ACAGTCAGCTGCTGGAATCATATAGGTCATTAACCCTTGGCTATATGGCAGAAGCGTTTGGTGTTGGTGT
  GGAATTCATTGATCAGGAACTGTCCAGGTTTATTGCTGCCGGGAGACTACACTGCAAAATAGATAAAGTG
  AATGAAATAGTAGAAACCAACAGACCTGATAGCAAGAACTGGCAGTACCAAGAAACTATCAAGAAAGGAG
  ATCTGCTACTAAACAGAGTTCAAAAACTTTCCAGAGTAATTAATATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202292 representing NM_014814
  Red=Cloning site Green=Tags(s)
  
  MPLENLEEEGLPKNPDLRIAQLRFLLSLPEHRGDAAVRDELMAAVRDNNMAPYYEALCKSLDWQIDVDLL
  NKMKKANEDELKRLDEELEDAEKNLGESEIRDAMMAKAEYLCRIGDKEGALTAFRKTYDKTVALGHRLDI
  VFYLLRIGLFYMDNDLITRNTEKAKSLIEEGGDWDRRNRLKVYQGLYCVAIRDFKQAAELFLDTVSTFTS
  YELMDYKTFVTYTVYVSMIALERPDLREKVIKGAEILEVLHSLPAVRQYLFSLYECRYSVFFQSLAVVEQ
  EMKKDWLFAPHYRYYVREMRIHAYSQLLESYRSLTLGYMAEAFGVGVEFIDQELSRFIAAGRLHCKIDKV
  NEIVETNRPDSKNWQYQETIKKGDLLLNRVQKLSRVINM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014814
• ORF Size: 1167 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014814.3
• RefSeq Size: 1308 bp
• RefSeq ORF: 1170 bp
• Locus ID: 9861
• UniProt ID: Q15008
• Cytogenetics: 3p14.1
• Domains: PCI
• Protein Pathways: Proteasome
• MW: 45.4 kDa
• Gene Summary: This gene encodes a member of the protease subunit S10 family. The encoded protein is a subunit of the 26S proteasome which colocalizes with DNA damage foci and is involved in the ATP-dependent degradation of ubiquinated proteins. Alternative splicing results in multiple transcript variants [provided by RefSeq, Nov 2012]