PSMD6 (NM_014814) Human Untagged Clone

CAT#: SC114839

PSMD6 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 6 (PSMD6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMD6
• Synonyms: p42A; p44S10; Rpn7; S10; SGA-113M
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114839 sequence for NM_014814 edited (data generated by NextGen Sequencing)
  ATGCCGCTGGAGAACCTGGAGGAGGAGGGTCTGCCCAAGAACCCCGACTTGCGTATCGCG
  CAGCTGCGCTTCCTGCTCAGCCTGCCCGAGCACCGCGGAGACGCTGCCGTGCGCGACGAG
  CTGATGGCGGCCGTCCGCGATAACAACATGGCTCCTTACTATGAAGCCTTGTGCAAATCC
  CTCGACTGGCAGATAGACGTGGACCTACTCAATAAAATGAAGAAGGCAAATGAAGATGAG
  TTGAAGCGTTTGGATGAGGAGCTGGAAGATGCAGAGAAGAATCTAGGAGAGAGCGAAATT
  CGCGATGCAATGATGGCAAAGGCCGAGTACCTCTGCCGGATAGGTGACAAAGAGGGAGCT
  CTGACAGCCTTTCGCAAGACATATGACAAAACTGTGGCCCTGGGTCACCGATTGGATATT
  GTATTCTATCTCCTTAGGATTGGCTTATTTTATATGGATAATGATCTCATCACACGAAAC
  ACAGAAAAGGCCAAAAGCTTAATAGAAGAAGGAGGAGACTGGGACAGGAGAAACCGCCTA
  AAAGTGTATCAGGGTCTTTATTGTGTGGCTATTCGTGATTTCAAACAGGCAGCTGAACTC
  TTCCTTGACACTGTTTCAACATTTACATCCTATGAACTCATGGATTATAAAACATTTGTG
  ACTTATACTGTCTATGTCAGTATGATTGCCTTAGAAAGACCAGATCTCAGGGAAAAGGTC
  ATTAAAGGAGCAGAGATTCTTGAAGTGTTGCACAGTCTTCCAGCAGTTCGGCAGTATCTG
  TTTTCACTCTATGAATGCCGTTACTCTGTTTTCTTCCAATCATTAGCGGTTGTGGAACAG
  GAAATGAAAAAGGACTGGCTTTTTGCCCCTCATTATCGATACTATGTAAGAGAAATGAGA
  ATTCATGCATACAGTCAGCTGCTGGAATCATATAGGTCATTAACCCTTGGCTATATGGCA
  GAAGCGTTTGGTGTTGGTGTGGAATTCATTGATCAGGAACTGTCCAGGTTTATTGCTGCC
  GGGAGACTACACTGCAAAATAGATAAAGTGAATGAAATAGTAGAAACCAACAGACCTGAT
  AGCAAGAACTGGCAGTACCAAGAAACTATCAAGAAAGGAGATCTGCTACTAAACAGAGTT
  CAAAAACTTTCCAGAGTAATTAATATGTAA
  
  Clone variation with respect to NM_014814.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014814 unedited
  NTGTCGNAATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGGTCCCCTT
  CGCCGCGATGCCGCTGGAGAACCTGGAGGAGGAGGGTCTGCCCAAGAACCCCGACTTGCG
  TATCGCGCAGCTGCGCTTCCTGCTCAGCCTGCCCGAGCACCGCGGAGACGCTGCCGTGCG
  CGACGAGCTGATGGCGGCCGTCCGCGATAACAACATGGCTCCTTACTATGAAGCCTTGTG
  CAAATCCCTCGACTGGCAGATAGACGTGGACCTACTCAATAAAATGAAGAAGGCAAATGA
  AGATGAGTTGAAGCGTTTGGATGAGGAGCTGGAAGATGCAGAGAAGAATCTAGGAGAGAG
  CGAAATTCGCGATGCAATGATGGCAAAGGCCGAGTACCTCTGCCGGATAGGTGACAAAGA
  GGGAGCTCTGACAGCCTTTCGCAAGACATATGACAAAACTGTGGCCCTGGGTCACCGATT
  GGATATTGTATTCTATCTCCTTAGGATTGGCTTATTTTATATGGATAATGATCTCATCAC
  ACGAAACACAGAAAAGGCCAAAAGCTTAATAGAAGAAGGAGGAGACTGGGACAGGAGAAA
  CCGCCTAAAAGTGTATCAGGGTCTTTATTGTGTGGCTATTCGTGATTTCAAACAGGCAGC
  TGAACTCTTCCTTGACACTGTTTCAACATTTACATCCTATGAACTCATGGATTATAAAAC
  ATTTGTGACTTATACTGTCTATGTCAGTATGATTGCCTTAGAAAGACCAGATCTCAGGGA
  ANAGGTCATTAAAGGAGCAGAGATTCTTGGAGTGTTGCACAGTCTTCCAGCAGTTCGGCA
  GTATCTGTTTTCACTCTATGAATGCCGTTACTCCTGTTTCCTTCCATCATTAACGGGTGT
  GGAACAGGAAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014814 unedited
  ATGGACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAACAACAATGCCGTTTTTTTTTTATA
  CAGTTGACCTGGGCCCATTGGGAAATAAGCTTTAAAAATTCCAAATAATTTTTTTTAAAA
  CAAATCCTTTGGTAATTACATGGGTTTACATATTAATTACTCTGGAAAGTTTTTGAACTT
  TGTTTAATAACAAAACTCCTTTTTTGAAAATTTCTTGGGACTGCCAATTTTTGGTATCAG
  GGCTGGGGGGTTCTACTATTTCATTCACTTTATCTATTTTGCAGGGGAGTCTCCCGGGAG
  CAATAAACCTGGACAGGTCCTGATCAATGAATTCCACACCAACACCAAACGCTTTTGGCA
  TTTAGCCAAGGGGTAATGACCTATTTTATTCCCCCACCTGACTGGATGCATGAATTCTCA
  TTTTTTTTACATAAAATCGAAAAAGAGGGGCAAAAAGCCCGTCCTTTTTCATTTCCTGTT
  CCACAACCGCTTATGATTGGAAAAAAACAAAGTAACGGCTTTCATAAAGGGAAAACAAAT
  ACTGCCCAACTGCTGGAAGACTGGGCAACACTTCAAAAATCTCTGGTCCTTTAATGACCC
  TTTCCCTGAGAACTGGGCTTTTTAAGGGCATCCTACTGGCCTATACAGTTTTAAGTCCAC
  AATGTTTTATAATCCCTGAATTCCTAAGATGGGAAATGTTGAAACCCTGTCAAGGGAAAA
  GNTCACCTTGCCTGTTGGATTCCCGAATAGCCCCCCAATTAAACCCTGGTACCTTTTAGC
  GGGTTTTTCTGGCCCAAATTCTTCTTTTTTATAAGCTTTGGGCCTTTTTGGGGTTCGGGG
  AGGAAACATTTCCTTTAAATAACCCATTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014814
• Insert Size: 1340 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014814.1, NP_055629.1
• RefSeq Size: 1308 bp
• RefSeq ORF: 1170 bp
• Locus ID: 9861
• UniProt ID: Q15008
• Cytogenetics: 3p14.1
• Domains: PCI
• Protein Pathways: Proteasome
• Gene Summary: This gene encodes a member of the protease subunit S10 family. The encoded protein is a subunit of the 26S proteasome which colocalizes with DNA damage foci and is involved in the ATP-dependent degradation of ubiquinated proteins. Alternative splicing results in multiple transcript variants [provided by RefSeq, Nov 2012]
  Transcript Variant: This variant (2) lacks an exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.