TCIRG1 (NM_006019) Human Tagged ORF Clone

CAT#: RC202125

TCIRG1 (Myc-DDK-tagged)-Human T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3 (TCIRG1), transcript variant 1

Product Images

Circular map for RC202125

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TCIRG1 (Cat# RC202125, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TCIRG(Cat# TA590586). Positive lysates LY416888 (100ug) and LC416888 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY416888) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202125 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TCIRG1
• Synonyms: a3; Atp6i; ATP6N1C; ATP6V0A3; OC-116kDa; OC116; OPTB1; Stv1; TIRC7; Vph1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202125 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTCCATGTTCCGGAGCGAGGAGGTGGCCCTGGTCCAGCTCTTTCTGCCCACAGCGGCTGCCTACA
  CCTGCGTGAGTCGGCTGGGCGAGCTGGGCCTCGTGGAGTTCAGAGACCTCAACGCCTCGGTGAGCGCCTT
  CCAGAGACGCTTTGTGGTTGATGTTCGGCGCTGTGAGGAGCTGGAGAAGACCTTCACCTTCCTGCAGGAG
  GAGGTGCGGCGGGCTGGGCTGGTCCTGCCCCCGCCAAAGGGGAGGCTGCCGGCACCCCCACCCCGGGACC
  TGCTGCGCATCCAGGAGGAGACGGAGCGCCTGGCCCAGGAGCTGCGGGATGTGCGGGGCAACCAGCAGGC
  CCTGCGGGCCCAGCTGCACCAGCTGCAGCTCCACGCCGCCGTGCTACGCCAGGGCCATGAACCTCAGCTG
  GCAGCCGCCCACACAGATGGGGCCTCAGAGAGGACGCCCCTGCTCCAGGCCCCCGGGGGGCCGCACCAGG
  ACCTGAGGGTCAACTTTGTGGCAGGTGCCGTGGAGCCCCACAAGGCCCCTGCCCTAGAGCGCCTGCTCTG
  GAGGGCCTGCCGCGGCTTCCTCATTGCCAGCTTCAGGGAGCTGGAGCAGCCGCTGGAGCACCCCGTGACG
  GGCGAGCCAGCCACGTGGATGACCTTCCTCATCTCCTACTGGGGTGAGCAGATCGGACAGAAGATCCGCA
  AGATCACGGACTGCTTCCACTGCCACGTCTTCCCGTTTCTGCAGCAGGAGGAGGCCCGCCTCGGGGCCCT
  GCAGCAGCTGCAACAGCAGAGCCAGGAGCTGCAGGAGGTCCTCGGGGAGACAGAGCGGTTCCTGAGCCAG
  GTGCTAGGCCGGGTGCTGCAGCTGCTGCCGCCAGGGCAGGTGCAGGTCCACAAGATGAAGGCCGTGTACC
  TGGCCCTGAACCAGTGCAGCGTGAGCACCACGCACAAGTGCCTCATTGCCGAGGCCTGGTGCTCTGTGCG
  AGACCTGCCCGCCCTGCAGGAGGCCCTGCGGGACAGCTCGATGGAGGAGGGAGTGAGTGCCGTGGCTCAC
  CGCATCCCCTGCCGGGACATGCCCCCCACACTCATCCGCACCAACCGCTTCACGGCCAGCTTCCAGGGCA
  TCGTGGATGCCTACGGCGTGGGCCGCTACCAGGAGGTCAACCCCGCTCCCTACACCATCATCACCTTCCC
  CTTCCTGTTTGCTGTGATGTTCGGGGATGTGGGCCACGGGCTGCTCATGTTCCTCTTCGCCCTGGCCATG
  GTCCTTGCGGAGAACCGACCGGCTGTGAAGGCCGCGCAGAACGAGATCTGGCAGACTTTCTTCAGGGGCC
  GCTACCTGCTCCTGCTTATGGGCCTGTTCTCCATCTACACCGGCTTCATCTACAACGAGTGCTTCAGTCG
  CGCCACCAGCATCTTCCCCTCGGGCTGGAGTGTGGCCGCCATGGCCAACCAGTCTGGCTGGAGTGATGCA
  TTCCTGGCCCAGCACACGATGCTTACCCTGGATCCCAACGTCACCGGTGTCTTCCTGGGACCCTACCCCT
  TTGGCATCGATCCTATTTGGAGCCTGGCTGCCAACCACTTGAGCTTCCTCAACTCCTTCAAGATGAAGAT
  GTCCGTCATCCTGGGCGTCGTGCACATGGCCTTTGGGGTGGTCCTCGGAGTCTTCAACCACGTGCACTTT
  GGCCAGAGGCACCGGCTGCTGCTGGAGACGCTGCCGGAGCTCACCTTCCTGCTGGGACTCTTCGGTTACC
  TCGTGTTCCTAGTCATCTACAAGTGGCTGTGTGTCTGGGCTGCCAGGGCCGCCTCGGCCCCCAGCATCCT
  CATCCACTTCATCAACATGTTCCTCTTCTCCCACAGCCCCAGCAACAGGCTGCTCTACCCCCGGCAGGAG
  GTGGTCCAGGCCACGCTGGTGGTCCTGGCCTTGGCCATGGTGCCCATCCTGCTGCTTGGCACACCCCTGC
  ACCTGCTGCACCGCCACCGCCGCCGCCTGCGGAGGAGGCCCGCTGACCGACAGGAGGAAAACAAGGCCGG
  GTTGCTGGACCTGCCTGACGCATCTGTGAATGGCTGGAGCTCCGATGAGGAAAAGGCAGGGGGCCTGGAT
  GATGAAGAGGAGGCCGAGCTCGTCCCCTCCGAGGTGCTCATGCACCAGGCCATCCACACCATCGAGTTCT
  GCCTGGGCTGCGTCTCCAACACCGCCTCCTACCTGCGCCTGTGGGCCCTGAGCCTGGCCCACGCCCAGCT
  GTCCGAGGTTCTGTGGGCCATGGTGATGCGCATAGGCCTGGGCCTGGGCCGGGAGGTGGGCGTGGCGGCT
  GTGGTGCTGGTCCCCATCTTTGCCGCCTTTGCCGTGATGACCGTGGCTATCCTGCTGGTGATGGAGGGAC
  TCTCAGCCTTCCTGCACGCCCTGCGGCTGCACTGGGTGGAATTCCAGAACAAGTTCTACTCAGGCACGGG
  CTACAAGCTGAGTCCCTTCACCTTCGCTGCCACAGATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202125 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGSMFRSEEVALVQLFLPTAAAYTCVSRLGELGLVEFRDLNASVSAFQRRFVVDVRRCEELEKTFTFLQE
  EVRRAGLVLPPPKGRLPAPPPRDLLRIQEETERLAQELRDVRGNQQALRAQLHQLQLHAAVLRQGHEPQL
  AAAHTDGASERTPLLQAPGGPHQDLRVNFVAGAVEPHKAPALERLLWRACRGFLIASFRELEQPLEHPVT
  GEPATWMTFLISYWGEQIGQKIRKITDCFHCHVFPFLQQEEARLGALQQLQQQSQELQEVLGETERFLSQ
  VLGRVLQLLPPGQVQVHKMKAVYLALNQCSVSTTHKCLIAEAWCSVRDLPALQEALRDSSMEEGVSAVAH
  RIPCRDMPPTLIRTNRFTASFQGIVDAYGVGRYQEVNPAPYTIITFPFLFAVMFGDVGHGLLMFLFALAM
  VLAENRPAVKAAQNEIWQTFFRGRYLLLLMGLFSIYTGFIYNECFSRATSIFPSGWSVAAMANQSGWSDA
  FLAQHTMLTLDPNVTGVFLGPYPFGIDPIWSLAANHLSFLNSFKMKMSVILGVVHMAFGVVLGVFNHVHF
  GQRHRLLLETLPELTFLLGLFGYLVFLVIYKWLCVWAARAASAPSILIHFINMFLFSHSPSNRLLYPRQE
  VVQATLVVLALAMVPILLLGTPLHLLHRHRRRLRRRPADRQEENKAGLLDLPDASVNGWSSDEEKAGGLD
  DEEEAELVPSEVLMHQAIHTIEFCLGCVSNTASYLRLWALSLAHAQLSEVLWAMVMRIGLGLGREVGVAA
  VVLVPIFAAFAVMTVAILLVMEGLSAFLHALRLHWVEFQNKFYSGTGYKLSPFTFAATDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006019
• ORF Size: 2490 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006019.3
• RefSeq Size: 2727 bp
• RefSeq ORF: 2493 bp
• Locus ID: 10312
• UniProt ID: Q13488
• Cytogenetics: 11q13.2
• Domains: V_ATPase_sub_a
• Protein Families: Transmembrane
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
• MW: 93 kDa
• Gene Summary: This gene encodes a subunit of a large protein complex known as a vacuolar H+-ATPase (V-ATPase). The protein complex acts as a pump to move protons across the membrane. This movement of protons helps regulate the pH of cells and their surrounding environment. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. Alternative splicing results in multiple transcript variants. Mutations in this gene are associated with infantile malignant osteopetrosis. [provided by RefSeq, May 2017]