TCIRG1 Rabbit Polyclonal Antibody

CAT#: TA382379

TCIRG1 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using TCIRG1 antibody (TA382379) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

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  Immunofluorescence analysis of L929 cells using TCIRG1 Polyclonal Antibody (TA382379) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of U-2 OS cells using TCIRG1 Polyclonal Antibody (TA382379) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:200 - 1:2000
  IHC,1:50 - 1:200
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human TCIRG1 (NP_006010.2).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 68kDa/92kDa
• Gene Name: T-cell immune regulator 1, ATPase H+ transporting V0 subunit a3
• Database Link:
  Entrez Gene 10312 Human
  UniProt ID Q13488
• Background: This gene encodes a subunit of a large protein complex known as a vacuolar H+-ATPase (V-ATPase). The protein complex acts as a pump to move protons across the membrane. This movement of protons helps regulate the pH of cells and their surrounding environment. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. Alternative splicing results in multiple transcript variants. Mutations in this gene are associated with infantile malignant osteopetrosis.
• Synonyms: a3; Atp6i; ATP6N1C; ATP6V0A3; OC-116; OC-116kDa; OC116; OPTB1; Stv1; TIRC7; Vph1