p27 KIP 1 (CDKN1B) (NM_004064) Human Tagged ORF Clone
CDKN1B (Myc-DDK-tagged)-Human cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B)
|Type||Human Tagged ORF Clone|
|Synonyms||CDKN4; KIP1; MEN1B; MEN4; P27KIP1|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC201661 representing NM_004064
Red=Cloning site Blue=ORF Green=Tags(s)
>RC201661 representing NM_004064
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||594 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||2422 bp|
|RefSeq ORF||597 bp|
|Protein Families||Druggable Genome|
|Protein Pathways||Cell cycle, Chronic myeloid leukemia, ErbB signaling pathway, Pathways in cancer, Prostate cancer, Small cell lung cancer|
|Gene Summary||This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]|
|cDNA Clone Resources|
|RC201661L1||Lenti ORF clone of Human cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B), Myc-DDK-tagged||
|RC201661L2||Lenti ORF clone of Human cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B), mGFP tagged||
|RC201661L3||Lenti ORF clone of Human cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B), Myc-DDK-tagged||
|RC201661L4||Lenti ORF clone of Human cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B), mGFP tagged||
|RG201661||CDKN1B (GFP-tagged) - Human cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B)||
|SC117607||CDKN1B (untagged)-Human cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B)||