ESD (NM_001984) Human Tagged ORF Clone

CAT#: RC200533

ESD (Myc-DDK-tagged)-Human esterase D (ESD)

Product Images

Western blot validation of overexpression lysate (Cat# LY419606) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200533 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ESD protein (Cat# TP300533). The protein was produced from HEK293T cells transfected with ESD cDNA clone (Cat# RC200533) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ESD
• Synonyms: FGH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200533 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATTGAAGCAGATTTCCAGCAACAAGTGCTTTGGGGGATTGCAGAAAGTTTTTGAACATGACAGTG
  TTGAACTAAACTGCAAAATGAAATTTGCTGTCTACTTACCACCAAAGGCAGAAACAGGAAAGTGCCCTGC
  ACTGTATTGGCTCTCAGGTTTAACTTGCACAGAGCAAAATTTTATATCAAAATCTGGTTATCATCAGTCT
  GCTTCAGAACATGGTCTTGTTGTCATTGCTCCAGATACCAGCCCTCGTGGCTGCAATATTAAAGGTGAAG
  ATGAGAGCTGGGACTTTGGCACTGGTGCTGGATTTTATGTTGATGCCACTGAAGATCCTTGGAAAACCAA
  CTACAGAATGTACTCTTATGTCACAGAGGAGCTTCCCCAACTCATAAATGCCAATTTTCCAGTGGATCCC
  CAAAGGATGTCTATTTTTGGCCACTCCATGGGAGGTCATGGAGCTCTGATCTGTGCTTTGAAAAATCCTG
  GAAAATACAAATCTGTGTCAGCATTTGCTCCAATTTGCAACCCTGTACTCTGTCCCTGGGGCAAAAAAGC
  CTTTAGTGGATATTTGGGAACAGATCAAAGTAAATGGAAGGCTTATGATGCTACCCACCTTGTGAAATCC
  TATCCAGGATCTCAGCTGGACATACTAATTGATCAAGGGAAAGATGACCAGTTTCTTTTAGATGGACAGT
  TACTCCCTGATAACTTCATAGCTGCCTGTACAGAAAAGAAAATCCCCGTTGTTTTTCGATTGCAAGAGGA
  TTATGATCATAGCTACTACTTCATTGCAACCTTTATTACTGACCACATCAGACATCATGCTAAATACCTG
  AATGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200533 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALKQISSNKCFGGLQKVFEHDSVELNCKMKFAVYLPPKAETGKCPALYWLSGLTCTEQNFISKSGYHQS
  ASEHGLVVIAPDTSPRGCNIKGEDESWDFGTGAGFYVDATEDPWKTNYRMYSYVTEELPQLINANFPVDP
  QRMSIFGHSMGGHGALICALKNPGKYKSVSAFAPICNPVLCPWGKKAFSGYLGTDQSKWKAYDATHLVKS
  YPGSQLDILIDQGKDDQFLLDGQLLPDNFIAACTEKKIPVVFRLQEDYDHSYYFIATFITDHIRHHAKYL
  NA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001984
• ORF Size: 846 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001984.1, NP_001975.1
• RefSeq Size: 1208 bp
• RefSeq ORF: 849 bp
• Locus ID: 2098
• UniProt ID: P10768
• Cytogenetics: 13q14.2
• MW: 31.5 kDa
• Gene Summary: This gene encodes a serine hydrolase that belongs to the esterase D family. The encoded enzyme is active toward numerous substrates including O-acetylated sialic acids, and it may be involved in the recycling of sialic acids. This gene is used as a genetic marker for retinoblastoma and Wilson's disease. [provided by RefSeq, Feb 2009]