Drebrin (DBN1) (NM_004395) Human Tagged ORF Clone

CAT#: RC200372

DBN1 (Myc-DDK-tagged)-Human drebrin 1 (DBN1), transcript variant 1

Product Images

Circular map for RC200372

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DBN1 (Cat# RC200372, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DBN1 (Cat# TA812128). Positive lysates LY418021 (100ug) and LC418021 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY418021) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200372 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Drebrin
• Synonyms: D0S117E
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200372 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCGTCAGCTTCAGCGGCCACCGCCTGGAGCTGCTGGCGGCTTACGAGGAGGTGATCCGAGAGG
  AGAGCGCGGCCGACTGGGCTCTGTACACATATGAAGATGGCTCCGATGACCTCAAGCTTGCAGCATCAGG
  AGAAGGGGGCTTGCAGGAGCTTTCGGGACACTTTGAGAACCAGAAGGTGATGTACGGCTTCTGCAGTGTC
  AAGGACTCCCAAGCTGCTCTGCCAAAATACGTGCTCATCAACTGGGTGGGCGAAGATGTGCCTGATGCCC
  GCAAGTGCGCTTGTGCCAGCCACGTGGCTAAGGTGGCAGAGTTCTTCCAGGGTGTCGACGTGATCGTGAA
  CGCCAGCAGCGTGGAAGACATAGACGCGGGTGCCATCGGGCAGCGGCTCTCTAACGGGCTGGCGCGACTC
  TCCAGCCCTGTGCTGCACCGACTGCGGCTGCGAGAGGATGAGAACGCAGAGCCCGTGGGCACCACCTACC
  AGAAGACGGATGCAGCTGTGGAAATGAAGCGGATTAACCGAGAGCAGTTCTGGGAGCAGGCCAAGAAGGA
  AGAAGAGCTGCGGAAGGAGGAGGAGCGGAAGAAGGCCCTGGATGAGAGGCTCAGGTTCGAGCAGGAGCGG
  ATGGAGCAGGAGCGGCAGGAGCAAGAGGAGCGCGAGCGGCGCTACCGGGAGCGGGAGCAGCAGATCGAGG
  AGCACAGGAGGAAACAGCAGACTTTAGAAGCGGAAGAGGCCAAGAGGCGGTTGAAGGAGCAGTCTATCTT
  TGGTGACCATCGGGATGAGGAGGAAGAGACCCACATGAAGAAGTCAGAGTCGGAGGTGGAGGAGGCAGCA
  GCTATTATTGCCCAGCGGCCTGACAACCCAAGGGAGTTCTTCAAGCAGCAGGAAAGAGTCGCATCGGCCT
  CTGCGGGCAGCTGTGATGTACCCTCGCCCTTCAACCATCGACCAGGCAGCCACCTGGACAGCCACCGGAG
  GATGGCGCCCACTCCCATCCCCACGCGGAGCCCGTCTGACTCCAGCACCGCCTCCACCCCTGTCGCTGAG
  CAGATAGAGCGGGCCCTGGATGAGGTCACCTCCTCGCAGCCTCCACCACTGCCACCGCCACCCCCACCAG
  CCCAAGAGACCCAGGAGCCCAGCCCCATCCTAGACAGTGAGGAGACCAGAGCAGCAGCCCCTCAGGCCTG
  GGCCGGCCCCATGGAGGAGCCCCCTCAGGCACAGGCGCCTCCCCGGGGGCCAGGCAGCCCTGCAGAGGAC
  TTGATGTTCATGGAGTCTGCAGAGCAGGCTGTCCTGGCTGCTCCCGTGGAGCCTGCCACAGCTGACGCCA
  CGGAGGTCCACGATGCAGCTGACACCATTGAAACTGACACTGCCACTGCTGACACCACTGTTGCCAACAA
  CGTACCCCCCGCCGCCACCAGCCTCATTGACCTATGGCCTGGCAACGGGGAAGGGGCCTCCACACTCCAG
  GGTGAGCCCAGGGCCCCCACGCCACCCTCGGGTACTGAGGTCACCCTGGCAGAGGTGCCCCTGCTGGATG
  AGGTGGCTCCGGAGCCACTGCTGCCAGCAGGCGAAGGCTGTGCCACCCTTCTCAACTTTGATGAGCTGCC
  TGAGCCGCCAGCCACCTTCTGTGACCCAGAGGAAGTGGAAGGGGAGCCCCTGGCTGCCCCCCAGACCCCA
  ACTCTGCCCTCAGCCCTTGAGGAGCTGGAGCAAGAGCAGGAGCCGGAGCCCCACCTGCTAACCAATGGCG
  AGACCACCCAGAAGGAGGGGACCCAGGCCAGTGAGGGGTACTTCAGTCAATCACAGGAGGAGGAGTTTGC
  CCAATCGGAAGAGCTCTGTGCCAAGGCTCCGCCTCCTGTGTTCTACAACAAGCCTCCAGAGATCGACATC
  ACATGCTGGGATGCAGACCCAGTTCCAGAAGAGGAGGAGGGCTTCGAGGGTGGTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200372 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGVSFSGHRLELLAAYEEVIREESAADWALYTYEDGSDDLKLAASGEGGLQELSGHFENQKVMYGFCSV
  KDSQAALPKYVLINWVGEDVPDARKCACASHVAKVAEFFQGVDVIVNASSVEDIDAGAIGQRLSNGLARL
  SSPVLHRLRLREDENAEPVGTTYQKTDAAVEMKRINREQFWEQAKKEEELRKEEERKKALDERLRFEQER
  MEQERQEQEERERRYREREQQIEEHRRKQQTLEAEEAKRRLKEQSIFGDHRDEEEETHMKKSESEVEEAA
  AIIAQRPDNPREFFKQQERVASASAGSCDVPSPFNHRPGSHLDSHRRMAPTPIPTRSPSDSSTASTPVAE
  QIERALDEVTSSQPPPLPPPPPPAQETQEPSPILDSEETRAAAPQAWAGPMEEPPQAQAPPRGPGSPAED
  LMFMESAEQAVLAAPVEPATADATEVHDAADTIETDTATADTTVANNVPPAATSLIDLWPGNGEGASTLQ
  GEPRAPTPPSGTEVTLAEVPLLDEVAPEPLLPAGEGCATLLNFDELPEPPATFCDPEEVEGEPLAAPQTP
  TLPSALEELEQEQEPEPHLLTNGETTQKEGTQASEGYFSQSQEEEFAQSEELCAKAPPPVFYNKPPEIDI
  TCWDADPVPEEEEGFEGGD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004395
• ORF Size: 1947 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004395.1
• RefSeq Size: 2942 bp
• RefSeq ORF: 1950 bp
• Locus ID: 1627
• UniProt ID: Q16643
• Cytogenetics: 5q35.3
• Domains: ADF
• MW: 71.4 kDa
• Gene Summary: The protein encoded by this gene is a cytoplasmic actin-binding protein thought to play a role in the process of neuronal growth. It is a member of the drebrin family of proteins that are developmentally regulated in the brain. A decrease in the amount of this protein in the brain has been implicated as a possible contributing factor in the pathogenesis of memory disturbance in Alzheimer's disease. At least two alternative splice variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]