Drebrin (DBN1) (NM_004395) Human Tagged ORF Clone

CAT#: RC200372

Reviews ()
Write a review

DBN1 (Myc-DDK-tagged)-Human drebrin 1 (DBN1), transcript variant 1



Reconstitution Protocol

USD 604.00

USD 906.00

In Stock*

Size
    • 10 ug

Product images

Other products for "DBN1"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol DBN1
Synonyms D0S117E
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200372 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGCGTCAGCTTCAGCGGCCACCGCCTGGAGCTGCTGGCGGCTTACGAGGAGGTGATCCGAGAGG
AGAGCGCGGCCGACTGGGCTCTGTACACATATGAAGATGGCTCCGATGACCTCAAGCTTGCAGCATCAGG
AGAAGGGGGCTTGCAGGAGCTTTCGGGACACTTTGAGAACCAGAAGGTGATGTACGGCTTCTGCAGTGTC
AAGGACTCCCAAGCTGCTCTGCCAAAATACGTGCTCATCAACTGGGTGGGCGAAGATGTGCCTGATGCCC
GCAAGTGCGCTTGTGCCAGCCACGTGGCTAAGGTGGCAGAGTTCTTCCAGGGTGTCGACGTGATCGTGAA
CGCCAGCAGCGTGGAAGACATAGACGCGGGTGCCATCGGGCAGCGGCTCTCTAACGGGCTGGCGCGACTC
TCCAGCCCTGTGCTGCACCGACTGCGGCTGCGAGAGGATGAGAACGCAGAGCCCGTGGGCACCACCTACC
AGAAGACGGATGCAGCTGTGGAAATGAAGCGGATTAACCGAGAGCAGTTCTGGGAGCAGGCCAAGAAGGA
AGAAGAGCTGCGGAAGGAGGAGGAGCGGAAGAAGGCCCTGGATGAGAGGCTCAGGTTCGAGCAGGAGCGG
ATGGAGCAGGAGCGGCAGGAGCAAGAGGAGCGCGAGCGGCGCTACCGGGAGCGGGAGCAGCAGATCGAGG
AGCACAGGAGGAAACAGCAGACTTTAGAAGCGGAAGAGGCCAAGAGGCGGTTGAAGGAGCAGTCTATCTT
TGGTGACCATCGGGATGAGGAGGAAGAGACCCACATGAAGAAGTCAGAGTCGGAGGTGGAGGAGGCAGCA
GCTATTATTGCCCAGCGGCCTGACAACCCAAGGGAGTTCTTCAAGCAGCAGGAAAGAGTCGCATCGGCCT
CTGCGGGCAGCTGTGATGTACCCTCGCCCTTCAACCATCGACCAGGCAGCCACCTGGACAGCCACCGGAG
GATGGCGCCCACTCCCATCCCCACGCGGAGCCCGTCTGACTCCAGCACCGCCTCCACCCCTGTCGCTGAG
CAGATAGAGCGGGCCCTGGATGAGGTCACCTCCTCGCAGCCTCCACCACTGCCACCGCCACCCCCACCAG
CCCAAGAGACCCAGGAGCCCAGCCCCATCCTAGACAGTGAGGAGACCAGAGCAGCAGCCCCTCAGGCCTG
GGCCGGCCCCATGGAGGAGCCCCCTCAGGCACAGGCGCCTCCCCGGGGGCCAGGCAGCCCTGCAGAGGAC
TTGATGTTCATGGAGTCTGCAGAGCAGGCTGTCCTGGCTGCTCCCGTGGAGCCTGCCACAGCTGACGCCA
CGGAGGTCCACGATGCAGCTGACACCATTGAAACTGACACTGCCACTGCTGACACCACTGTTGCCAACAA
CGTACCCCCCGCCGCCACCAGCCTCATTGACCTATGGCCTGGCAACGGGGAAGGGGCCTCCACACTCCAG
GGTGAGCCCAGGGCCCCCACGCCACCCTCGGGTACTGAGGTCACCCTGGCAGAGGTGCCCCTGCTGGATG
AGGTGGCTCCGGAGCCACTGCTGCCAGCAGGCGAAGGCTGTGCCACCCTTCTCAACTTTGATGAGCTGCC
TGAGCCGCCAGCCACCTTCTGTGACCCAGAGGAAGTGGAAGGGGAGCCCCTGGCTGCCCCCCAGACCCCA
ACTCTGCCCTCAGCCCTTGAGGAGCTGGAGCAAGAGCAGGAGCCGGAGCCCCACCTGCTAACCAATGGCG
AGACCACCCAGAAGGAGGGGACCCAGGCCAGTGAGGGGTACTTCAGTCAATCACAGGAGGAGGAGTTTGC
CCAATCGGAAGAGCTCTGTGCCAAGGCTCCGCCTCCTGTGTTCTACAACAAGCCTCCAGAGATCGACATC
ACATGCTGGGATGCAGACCCAGTTCCAGAAGAGGAGGAGGGCTTCGAGGGTGGTGAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200372 protein sequence
Red=Cloning site Green=Tags(s)

MAGVSFSGHRLELLAAYEEVIREESAADWALYTYEDGSDDLKLAASGEGGLQELSGHFENQKVMYGFCSV
KDSQAALPKYVLINWVGEDVPDARKCACASHVAKVAEFFQGVDVIVNASSVEDIDAGAIGQRLSNGLARL
SSPVLHRLRLREDENAEPVGTTYQKTDAAVEMKRINREQFWEQAKKEEELRKEEERKKALDERLRFEQER
MEQERQEQEERERRYREREQQIEEHRRKQQTLEAEEAKRRLKEQSIFGDHRDEEEETHMKKSESEVEEAA
AIIAQRPDNPREFFKQQERVASASAGSCDVPSPFNHRPGSHLDSHRRMAPTPIPTRSPSDSSTASTPVAE
QIERALDEVTSSQPPPLPPPPPPAQETQEPSPILDSEETRAAAPQAWAGPMEEPPQAQAPPRGPGSPAED
LMFMESAEQAVLAAPVEPATADATEVHDAADTIETDTATADTTVANNVPPAATSLIDLWPGNGEGASTLQ
GEPRAPTPPSGTEVTLAEVPLLDEVAPEPLLPAGEGCATLLNFDELPEPPATFCDPEEVEGEPLAAPQTP
TLPSALEELEQEQEPEPHLLTNGETTQKEGTQASEGYFSQSQEEEFAQSEELCAKAPPPVFYNKPPEIDI
TCWDADPVPEEEEGFEGGD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004395
ORF Size 1947 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004395.1
RefSeq Size 2942 bp
RefSeq ORF 1950 bp
Locus ID 1627
UniProt ID Q16643
Cytogenetics 5q35.3
Domains ADF
MW 71.4 kDa
Gene Summary The protein encoded by this gene is a cytoplasmic actin-binding protein thought to play a role in the process of neuronal growth. It is a member of the drebrin family of proteins that are developmentally regulated in the brain. A decrease in the amount of this protein in the brain has been implicated as a possible contributing factor in the pathogenesis of memory disturbance in Alzheimer's disease. At least two alternative splice variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.