Cad (NM_001289522) Mouse Tagged ORF Clone

CAT#: MR231918

Cad (myc-DDK-tagged) - Mouse carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant 2

Product Images

Circular map for MR231918

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cad
• Synonyms: 2410008J01Rik; AU018859; Cpad
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231918 representing NM_001289522
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCTGGTGTTGGAGGACGGGTCGGTGCTGCAGGGCCGGCCCTTTGGGGCCGCTGTGTCGACTG
  CTGGGGAAGTGGTGTTTCAGACCGGCATGGTCGGCTACCCAGAGGCCCTCACTGACCCTTCCTACAAAGC
  TCAGATCCTAGTGCTAACGTATCCTCTCATCGGCAACTACGGCATTCCCTCAGATGAAGAGGACGAGTTC
  GGCCTGAGCAAGTGGTTTGAATCCTCGGAGATCCACGTGGCGGGACTTGTAGTGGGAGAATGCTGTCCCA
  CACCCAGCCACTGGAGTGCCAACTGCACCCTGCATGAGTGGCTGCAACAGCGTGGCATCCCCGGCCTGCA
  AGGAGTGGACACTCGGGAGCTGACTAAGAAGTTGCGGGAGCAAGGGTCTCTTTTGGGGAAGCTGGTCCAG
  AAAGGGACAGAGCCGTCAGCCCTGCCATTCGTGGACCCCAATGCCAGGCCCCTGGCACCAGAGGTCTCTA
  TTAAGACTCCACGGGTATTCAATGCAGGGGGTGCCCCTCGGATCTGTGCTCTGGATTGTGGCCTCAAGTA
  TAATCAGATCCGATGTCTCTGCCAGCTTGGGGCTGAAGTTACCGTGGTACCCTGGGACCACGAGTTAGAC
  AGTCAGAAATATGACGGTCTCTTCCTAAGTAATGGACCCGGTGACCCTGCCTCTTACCCGGGTGTGGTGT
  CCACACTGAGCCGAGTCTTGTCTGAACCTAACCCCCGACCTGTGTTTGGAATCTGCCTTGGACACCAGCT
  GTTGGCTTTAGCCATCGGGGCCAAAACTTACAAGATGAGATACGGAAACCGAGGCCACAACCAGCCCTGT
  TTACTGGTGGGCACTGGGCGCTGCTTTCTGACGTCCCAGAATCATGGGTTTGCAGTGGACGCAGATTCGC
  TGCCAGCAGGCTGGGCTCCTCTGTTCACCAATGCCAATGACTGTTCCAATGAAGGCATAGTGCACGACAG
  CCTGCCCTTCTTCAGTGTCCAGTTTCACCCAGAGCACCGGGCCGGCCCTTCAGATATGGAACTGCTCTTC
  GATGTATTTCTAGAAACTGTGAGAGAGGCTGCAGCTGGGAACATTGGGGGCCAGACAGTAAGAGAACGCT
  TGGCTCAGCGCCTCTGTCCCCCTGAGCTCCCCATTCCTGGTTCTGGGCTCCCGCCTCCACGAAAGGTTCT
  AATCCTAGGCTCAGGGGGCCTCTCCATTGGCCAAGCTGGAGAATTTGACTACTCGGGCTCTCAGGCAATT
  AAAGCCCTAAAGGAGGAAAACATCCAGACATTACTAATCAACCCCAACATTGCCACAGTGCAGACTTCTC
  AGGGGCTGGCAGACAAGGTCTATTTCCTTCCCATAACACTTCACTACGTAACCCAGGTAATTCGTAATGA
  ACGCCCAGATGGTGTGTTACTGACTTTTGGGGGTCAAACAGCCCTGAACTGCGGTGTGGAGCTGACCAAA
  GCTGGGGTACTGGCTCGATATGGGGTCCGGGTCTTGGGTACACCCGTGGAGACCATTGAACTGACTGAGG
  ACCGACGAGCCTTCGCTGCCAGGATGGCTGAGATCGGAGAGCATGTCGCCCCCAGTGAAGCGGCAAATTC
  TCTTGAACAGGCTCAGGCAGCTGCCGAGCGACTAGGCTACCCTGTGCTGGTGCGAGCAGCCTTTGCCCTG
  GGTGGTCTCGGTTCTGGCTTTGCTTCCACCAAAGAGGAACTCTCAGCTCTTGTGGCTCCAGCTTTTGCCC
  ATACCAGTCAGGTGCTGATAGACAAGTCTCTGAAGGGCTGGAAGGAGATTGAATATGAGGTGGTGAGAGA
  CGCCTATGGCAACTGCGTAACGGTATGCAACATGGAGAACTTAGACCCACTGGGCATTCATACTGGTGAA
  TCCATAGTGGTGGCTCCGAGCCAGACACTGAATGACAGAGAGTACCAGCTTCTGCGACGGACAGCTATCA
  AGGTCACTCAGCACCTGGGCATTGTCGGGGAGTGCAATGTACAGTATGCTTTGAACCCCGAGTCTGAGCA
  GTATTACATCATTGAAGTGAATGCCAGGCTCTCCCGAAGCTCTGCCCTGGCCAGTAAGGCCACAGGCTAT
  CCACTAGCCTATGTGGCAGCCAAGCTAGCATTGGGCATTCCCCTGCCAGAGCTCAGAAACTCTGTTACGG
  GAGGAACAGCAGCCTTTGAGCCCAGCCTGGACTACTGCGTGGTAAAGATTCCTCGCTGGGACCTCAGCAA
  GTTCCTACGTGTCAGTACGAAGATTGGGAGTTGCATGAAGAGTGTTGGCGAGGTCATGGGCATCGGGCGG
  TCCTTTGAGGAGGCCTTCCAAAAGGCCCTGCGCATGGTCGATGAGAACTGCGTAGGCTTCGACCATACAG
  TAAAGCCAGTCAGCGACATGGAGCTGGAGACTCCGACGGATAAGCGGATCTTTGTGGTGGCTGCCGCTCT
  GTGGGCTGGCTACTCTGTGGAGCGCCTCTACGAGCTCACCCGCATTGATTGCTGGTTCCTGCACCGGATG
  AAGCGCATTGTGACCCACGCCCAGTTGCTGGAACAACACCGTGGACAAGCTTTACCCCAAGACCTGCTGC
  ACCAGGCCAAGTGCCTCGGCTTCTCAGACAAGCAGATTGCCCTGGCAGTCCTGAGCACAGAGCTGGCTGT
  TCGGAAGCTGCGTCAGGAACTGGGGATCTGCCCAGCGGTGAAACAGATTGACACAGTTGCAGCTGAGTGG
  CCAGCCCAGACAAATTACCTGTACCTGACATACTGGGGCAACACCCATGACCTCGACTTTCGGGCACCTC
  ACGTCCTGGTCCTTGGCTCTGGTGTCTACCGGATTGGCTCCAGTGTTGAGTTTGATTGGTGTGCTGTGGG
  TTGCATCCAGCAGCTCCGGAAGATGGGTTATAAGACCATCATGGTGAACTACAACCCAGAGACAGTCAGC
  ACCGACTATGATATGTGTGACCGACTCTACTTTGATGAGATCTCTTTTGAGGTGGTGATGGACATCTATG
  AGTTAGAGAACCCCGAAGGTGTGATCCTGTCCATGGGTGGGCAGCTGCCCAACAACATGGCCATGGCTTT
  GCATCGGCAGCAGTGCCGAGTGCTGGGCACCTCCCCCGAAGCCATCGACTCAGCCGAGAACCGATTCAAG
  TTCTCCCGGCTCCTAGATACCATCGGTATCAGCCAGCCTCAGTGGCGAGAGCTCAGTGACCTTGAGTCTG
  CTCGCCAGTTCTGCCACACGGTCGGGTACCCCTGCGTAGTGCGCCCCTCCTATGTGCTCAGCGGTGCTGC
  TATGAATGTGGCGTACACCGACGGGGACCTGGAGCGTTTCCTGAGCAGTGCGGCCGCCGTCTCTAAGGAG
  CACCCCGTAGTCATCTCCAAGTTCATTCAGGAAGCAAAGGAGATCGACGTGGACGCGGTGGCCTGTGACG
  GCATCGTGTCGGCCATTGCCATCTCTGAGCACGTGGAGAATGCAGGTGTGCATTCAGGTGATGCCACGCT
  GGTGACCCCCCCACAAGACATCACCCCGAAAACTCTGGAGCGGATCAAAGCCATTGTGCATGCTGTGGGC
  CAGGAGCTACAGGTTACAGGACCCTTCAATCTGCAGCTCATTGCCAAGGATGACCAGCTGAAGGTTATTG
  AATGCAACGTGCGTGTATCTCGCTCTTTCCCCTTCGTGTCCAAGACACTGGGTGTTGATCTAGTAGCCTT
  GGCCACAAGGATCATCATGGGGGAGAAAGTAGAGCCCGTGGGGCTCATGACTGGCTCTGGAGTCGTGGGA
  GTAAAGGTACCTCAGTTCTCCTTCTCACGCTTGGCCGGTGCCGACGTGGTACTTGGTGTGGAGATGACCA
  GTACTGGAGAGGTGGCCGGCTTCGGAGAGAGCCGTTGTGAAGCCTACCTCAAAGCCATGCTCAGCACTGG
  CTTTAAGATCCCAGAGAAGAACATCCTGCTGACTATTGGCAGCTACAAGAACAAAAGTGAGCTGCTCCCA
  ACTGTGCGGTTGCTGGAGAGCCTGGGCTACAGCCTCTATGCCAGCCTGGGTACGGCAGACTTCTACACTG
  AGCACGGGGTCAAGGTAACAGCTGTGGACTGGCACTTTGAAGAGGCGGTGGATGGTGAGTGCCCACCACA
  ACGGAGCATCTTGGATCAGCTGGCTGAAAATCACTTTGAGCTAGTGATTAACCTGTCCATGCGTGGGGCT
  GGGGGTCGGCGTCTTTCCTCCTTCGTTACCAAGGGCTACCGTACCCGGCGTCTGGCCGCTGATTTCTCTG
  TGCCTCTCATCATTGACATCAAGTGCACCAAACTCTTTGTGGAGGCCCTAGGCCAGATCGGCCCAGCCCC
  TCCTTTGAAAGTGCACGTTGACTGCATGACCTCCCAGAAGCTAGTGCGCCTGCCCGGATTGATTGATGTC
  CACGTGCACCTTCGGGAACCAGGTGGGACACACAAAGAGGACTTTGCCTCAGGCACAGCTGCTGCCTTGG
  CTGGGGGTGTCACCATGGTTTGTGCTATGCCTAATACCCGACCCCCCATCATTGATGCTCCTGCTCTCGC
  CCTTGCCCAGAAGCTGGCAGAGGCTGGCGCCCGCTGTGACTTTACCTTATTCCTTGGAGCCTCATCTGAG
  AACGCGGGGACTCTGGGTGCTGTGGCCGGCTCTGCAGCAGGCCTGAAGCTCTACCTCAACGAGACCTTCT
  CTGAGCTGCGGCTGGACAGTGTGGCCCAGTGGATGGAGCATTTTGAAACCTGGCCTGCCCACCTCCCCAT
  TGTGGCCCATGCAGAGCGGCAGAGTGTCGCTGCGGTCCTCATGGTGGCTCAGCTGACCCAGCGTCCAGTG
  CACATATGTCATGTGGCTCGGAAGGAAGAGATCCTGTTGATTAAAACTGCAAAGGCGCAGGGGCTGCCAG
  TGACCTGTGAGGTCGCTCCTCACCACCTGTTCCTAAACCGGGAAGACTTGGAGCGCCTGGGACCTGGGAA
  GGGAGAGGTCCGTCCTGAGCTTGGGTCCCGAGAGGATATGGAAGCCCTGTGGGAGAATATGGCAGTCATC
  GACTGTTTTGCCTCAGACCACGCTCCCCATACCTTGGAAGAGAAGTGTGGGCCCAAGCCTCCACCTGGCT
  TCCCGGGCCTAGAGACCATGTTGCCGCTGCTGCTGACAGCTGTGAGCGAGGGCCGCCTCAGTCTGGATGA
  CCTGCTGCAGCGATTGCACCACAACCCTCGGCGGATCTTCCACCTGCCTCTCCAGGAGGACACCTATGTA
  GAGGTGGATCTGGAGCATGAGTGGACAGTCCCCAGCCACATGCCCTTCTCTAAGGCCCGCTGGACCCCAT
  TTGAAGGACAGAAGGTGAAGGGCACAGTGCGCCGTGTGGTCTTGCGAGGGGAGGTCGCCTATATTGATGG
  ACAGGTGCTGGTACCCCCGGGCTATGGACAAGATGTACGGAAGTGGCCGCAGGGGGTTGTTCCCCAGCCC
  CCTCCTTCAACTCCCGCCACCACCGAAATCACCACGACACCTGAGCGACCACGCCGAGTCATCCCGGGCC
  TTCCTGATGGCCGCTTCCACCTGCCACCCCGCATCCACCGGGCCTCTGACCCAGGTCTGCCAGCTGAGGA
  ACCTAAAGAGAAGCCACCCAGGAAGGTAGTGGAACCAGAGCTGATGGGAACCCCTGATGGTCCCTGCTAC
  CCTGCACCACCAGTACCTAGACAGGCATCACCCCAGAACCTAGGGTCTTCTGGCCTGCTGCACCCCCAGA
  TGTCGCCCCTACTGCACTCCCTAGTGGGCCAGCACATCCTGTCTGTCAAGCAGTTCACTAAGGATCAGAT
  GTCTCATCTGTTCAATGTGGCGCACACGCTACGGATGATGGTACAGAAGGAGCGGAGCCTCGACATCCTG
  AAGCTGGCAGCCAAGCACTGTCGCAGGCCAGTGATCAATGCTGGGGATGGAGTTGGGGAGCACCCCACAC
  AGGCCCTGCTGGACATCTTCACCATTCGAGAAGAGCTGGGGACTGTCAATGGCATGACGATCACTATGGT
  CGGTGACCTGAAGCATGGACGCACAGTGCACTCCCTGGCCTGCCTGCTCACCCAGTACCGTGTGAGCCTA
  CGCTACGTGGCACCTCCCAGCCTGCGCATGCCACCCAGTGTACGGGACTTTGTGGCCTCCCGAGGCACCA
  AGCAGGAGGAGTTTGAGAGCATCGAGGAGGCGCTGCCCGACACAGACGTGCTCTACATGACTCGGATCCA
  GAAAGAACGATTTGGCTCTGTCCAGGAATATGAAGCTTGCTTTGGTCAGTTCATCCTCACTCCTCACATC
  ATGACCCGGGCCAAGAAGAAGATGGTGGTGATGCACCCAATGCCCCGTGTCAATGAGATAAGTGTGGAGG
  TGGACTCGGACCCCCGGGCAGCCTACTTCCGCCAAGCTGAGAACGGCATGTACATCCGCATGGCCCTGCT
  TGCCACCGTGCTTGGCCGTTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231918 representing NM_001289522
  Red=Cloning site Green=Tags(s)
  
  MAALVLEDGSVLQGRPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPSDEEDEF
  GLSKWFESSEIHVAGLVVGECCPTPSHWSANCTLHEWLQQRGIPGLQGVDTRELTKKLREQGSLLGKLVQ
  KGTEPSALPFVDPNARPLAPEVSIKTPRVFNAGGAPRICALDCGLKYNQIRCLCQLGAEVTVVPWDHELD
  SQKYDGLFLSNGPGDPASYPGVVSTLSRVLSEPNPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPC
  LLVGTGRCFLTSQNHGFAVDADSLPAGWAPLFTNANDCSNEGIVHDSLPFFSVQFHPEHRAGPSDMELLF
  DVFLETVREAAAGNIGGQTVRERLAQRLCPPELPIPGSGLPPPRKVLILGSGGLSIGQAGEFDYSGSQAI
  KALKEENIQTLLINPNIATVQTSQGLADKVYFLPITLHYVTQVIRNERPDGVLLTFGGQTALNCGVELTK
  AGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFAL
  GGLGSGFASTKEELSALVAPAFAHTSQVLIDKSLKGWKEIEYEVVRDAYGNCVTVCNMENLDPLGIHTGE
  SIVVAPSQTLNDREYQLLRRTAIKVTQHLGIVGECNVQYALNPESEQYYIIEVNARLSRSSALASKATGY
  PLAYVAAKLALGIPLPELRNSVTGGTAAFEPSLDYCVVKIPRWDLSKFLRVSTKIGSCMKSVGEVMGIGR
  SFEEAFQKALRMVDENCVGFDHTVKPVSDMELETPTDKRIFVVAAALWAGYSVERLYELTRIDCWFLHRM
  KRIVTHAQLLEQHRGQALPQDLLHQAKCLGFSDKQIALAVLSTELAVRKLRQELGICPAVKQIDTVAAEW
  PAQTNYLYLTYWGNTHDLDFRAPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVS
  TDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFK
  FSRLLDTIGISQPQWRELSDLESARQFCHTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKE
  HPVVISKFIQEAKEIDVDAVACDGIVSAIAISEHVENAGVHSGDATLVTPPQDITPKTLERIKAIVHAVG
  QELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRIIMGEKVEPVGLMTGSGVVG
  VKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPEKNILLTIGSYKNKSELLP
  TVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILDQLAENHFELVINLSMRGA
  GGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDV
  HVHLREPGGTHKEDFASGTAAALAGGVTMVCAMPNTRPPIIDAPALALAQKLAEAGARCDFTLFLGASSE
  NAGTLGAVAGSAAGLKLYLNETFSELRLDSVAQWMEHFETWPAHLPIVAHAERQSVAAVLMVAQLTQRPV
  HICHVARKEEILLIKTAKAQGLPVTCEVAPHHLFLNREDLERLGPGKGEVRPELGSREDMEALWENMAVI
  DCFASDHAPHTLEEKCGPKPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPLQEDTYV
  EVDLEHEWTVPSHMPFSKARWTPFEGQKVKGTVRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGVVPQP
  PPSTPATTEITTTPERPRRVIPGLPDGRFHLPPRIHRASDPGLPAEEPKEKPPRKVVEPELMGTPDGPCY
  PAPPVPRQASPQNLGSSGLLHPQMSPLLHSLVGQHILSVKQFTKDQMSHLFNVAHTLRMMVQKERSLDIL
  KLAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSL
  RYVAPPSLRMPPSVRDFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSVQEYEACFGQFILTPHI
  MTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289522
• ORF Size: 6462 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289522.1, NP_001276451.1
• RefSeq Size: 6924 bp
• RefSeq ORF: 6465 bp
• Locus ID: 69719
• UniProt ID: B2RQC6
• Cytogenetics: 5 B1
• MW: 236.3 kDa
• Gene Summary: This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).[UniProtKB/Swiss-Prot Function]