Cad (NM_001289522) Mouse Untagged Clone

CAT#: MC229707

Cad (untagged) - Mouse carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cad
• Synonyms: 2410008J01Rik; AU018859; Cpad
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229707 representing NM_001289522
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCTGGTGTTGGAGGACGGGTCGGTGCTGCAGGGCCGGCCCTTTGGGGCCGCTGTGTCGACTG
  CTGGGGAAGTGGTGTTTCAGACCGGCATGGTCGGCTACCCAGAGGCCCTCACTGACCCTTCCTACAAAGC
  TCAGATCCTAGTGCTAACGTATCCTCTCATCGGCAACTACGGCATTCCCTCAGATGAAGAGGACGAGTTC
  GGCCTGAGCAAGTGGTTTGAATCCTCGGAGATCCACGTGGCGGGACTTGTAGTGGGAGAATGCTGTCCCA
  CACCCAGCCACTGGAGTGCCAACTGCACCCTGCATGAGTGGCTGCAACAGCGTGGCATCCCCGGCCTGCA
  AGGAGTGGACACTCGGGAGCTGACTAAGAAGTTGCGGGAGCAAGGGTCTCTTTTGGGGAAGCTGGTCCAG
  AAAGGGACAGAGCCGTCAGCCCTGCCATTCGTGGACCCCAATGCCAGGCCCCTGGCACCAGAGGTCTCTA
  TTAAGACTCCACGGGTATTCAATGCAGGGGGTGCCCCTCGGATCTGTGCTCTGGATTGTGGCCTCAAGTA
  TAATCAGATCCGATGTCTCTGCCAGCTTGGGGCTGAAGTTACCGTGGTACCCTGGGACCACGAGTTAGAC
  AGTCAGAAATATGACGGTCTCTTCCTAAGTAATGGACCCGGTGACCCTGCCTCTTACCCGGGTGTGGTGT
  CCACACTGAGCCGAGTCTTGTCTGAACCTAACCCCCGACCTGTGTTTGGAATCTGCCTTGGACACCAGCT
  GTTGGCTTTAGCCATCGGGGCCAAAACTTACAAGATGAGATACGGAAACCGAGGCCACAACCAGCCCTGT
  TTACTGGTGGGCACTGGGCGCTGCTTTCTGACGTCCCAGAATCATGGGTTTGCAGTGGACGCAGATTCGC
  TGCCAGCAGGCTGGGCTCCTCTGTTCACCAATGCCAATGACTGTTCCAATGAAGGCATAGTGCACGACAG
  CCTGCCCTTCTTCAGTGTCCAGTTTCACCCAGAGCACCGGGCCGGCCCTTCAGATATGGAACTGCTCTTC
  GATGTATTTCTAGAAACTGTGAGAGAGGCTGCAGCTGGGAACATTGGGGGCCAGACAGTAAGAGAACGCT
  TGGCTCAGCGCCTCTGTCCCCCTGAGCTCCCCATTCCTGGTTCTGGGCTCCCGCCTCCACGAAAGGTTCT
  AATCCTAGGCTCAGGGGGCCTCTCCATTGGCCAAGCTGGAGAATTTGACTACTCGGGCTCTCAGGCAATT
  AAAGCCCTAAAGGAGGAAAACATCCAGACATTACTAATCAACCCCAACATTGCCACAGTGCAGACTTCTC
  AGGGGCTGGCAGACAAGGTCTATTTCCTTCCCATAACACTTCACTACGTAACCCAGGTAATTCGTAATGA
  ACGCCCAGATGGTGTGTTACTGACTTTTGGGGGTCAAACAGCCCTGAACTGCGGTGTGGAGCTGACCAAA
  GCTGGGGTACTGGCTCGATATGGGGTCCGGGTCTTGGGTACACCCGTGGAGACCATTGAACTGACTGAGG
  ACCGACGAGCCTTCGCTGCCAGGATGGCTGAGATCGGAGAGCATGTCGCCCCCAGTGAAGCGGCAAATTC
  TCTTGAACAGGCTCAGGCAGCTGCCGAGCGACTAGGCTACCCTGTGCTGGTGCGAGCAGCCTTTGCCCTG
  GGTGGTCTCGGTTCTGGCTTTGCTTCCACCAAAGAGGAACTCTCAGCTCTTGTGGCTCCAGCTTTTGCCC
  ATACCAGTCAGGTGCTGATAGACAAGTCTCTGAAGGGCTGGAAGGAGATTGAATATGAGGTGGTGAGAGA
  CGCCTATGGCAACTGCGTAACGGTATGCAACATGGAGAACTTAGACCCACTGGGCATTCATACTGGTGAA
  TCCATAGTGGTGGCTCCGAGCCAGACACTGAATGACAGAGAGTACCAGCTTCTGCGACGGACAGCTATCA
  AGGTCACTCAGCACCTGGGCATTGTCGGGGAGTGCAATGTACAGTATGCTTTGAACCCCGAGTCTGAGCA
  GTATTACATCATTGAAGTGAATGCCAGGCTCTCCCGAAGCTCTGCCCTGGCCAGTAAGGCCACAGGCTAT
  CCACTAGCCTATGTGGCAGCCAAGCTAGCATTGGGCATTCCCCTGCCAGAGCTCAGAAACTCTGTTACGG
  GAGGAACAGCAGCCTTTGAGCCCAGCCTGGACTACTGCGTGGTAAAGATTCCTCGCTGGGACCTCAGCAA
  GTTCCTACGTGTCAGTACGAAGATTGGGAGTTGCATGAAGAGTGTTGGCGAGGTCATGGGCATCGGGCGG
  TCCTTTGAGGAGGCCTTCCAAAAGGCCCTGCGCATGGTCGATGAGAACTGCGTAGGCTTCGACCATACAG
  TAAAGCCAGTCAGCGACATGGAGCTGGAGACTCCGACGGATAAGCGGATCTTTGTGGTGGCTGCCGCTCT
  GTGGGCTGGCTACTCTGTGGAGCGCCTCTACGAGCTCACCCGCATTGATTGCTGGTTCCTGCACCGGATG
  AAGCGCATTGTGACCCACGCCCAGTTGCTGGAACAACACCGTGGACAAGCTTTACCCCAAGACCTGCTGC
  ACCAGGCCAAGTGCCTCGGCTTCTCAGACAAGCAGATTGCCCTGGCAGTCCTGAGCACAGAGCTGGCTGT
  TCGGAAGCTGCGTCAGGAACTGGGGATCTGCCCAGCGGTGAAACAGATTGACACAGTTGCAGCTGAGTGG
  CCAGCCCAGACAAATTACCTGTACCTGACATACTGGGGCAACACCCATGACCTCGACTTTCGGGCACCTC
  ACGTCCTGGTCCTTGGCTCTGGTGTCTACCGGATTGGCTCCAGTGTTGAGTTTGATTGGTGTGCTGTGGG
  TTGCATCCAGCAGCTCCGGAAGATGGGTTATAAGACCATCATGGTGAACTACAACCCAGAGACAGTCAGC
  ACCGACTATGATATGTGTGACCGACTCTACTTTGATGAGATCTCTTTTGAGGTGGTGATGGACATCTATG
  AGTTAGAGAACCCCGAAGGTGTGATCCTGTCCATGGGTGGGCAGCTGCCCAACAACATGGCCATGGCTTT
  GCATCGGCAGCAGTGCCGAGTGCTGGGCACCTCCCCCGAAGCCATCGACTCAGCCGAGAACCGATTCAAG
  TTCTCCCGGCTCCTAGATACCATCGGTATCAGCCAGCCTCAGTGGCGAGAGCTCAGTGACCTTGAGTCTG
  CTCGCCAGTTCTGCCACACGGTCGGGTACCCCTGCGTAGTGCGCCCCTCCTATGTGCTCAGCGGTGCTGC
  TATGAATGTGGCGTACACCGACGGGGACCTGGAGCGTTTCCTGAGCAGTGCGGCCGCCGTCTCTAAGGAG
  CACCCCGTAGTCATCTCCAAGTTCATTCAGGAAGCAAAGGAGATCGACGTGGACGCGGTGGCCTGTGACG
  GCATCGTGTCGGCCATTGCCATCTCTGAGCACGTGGAGAATGCAGGTGTGCATTCAGGTGATGCCACGCT
  GGTGACCCCCCCACAAGACATCACCCCGAAAACTCTGGAGCGGATCAAAGCCATTGTGCATGCTGTGGGC
  CAGGAGCTACAGGTTACAGGACCCTTCAATCTGCAGCTCATTGCCAAGGATGACCAGCTGAAGGTTATTG
  AATGCAACGTGCGTGTATCTCGCTCTTTCCCCTTCGTGTCCAAGACACTGGGTGTTGATCTAGTAGCCTT
  GGCCACAAGGATCATCATGGGGGAGAAAGTAGAGCCCGTGGGGCTCATGACTGGCTCTGGAGTCGTGGGA
  GTAAAGGTACCTCAGTTCTCCTTCTCACGCTTGGCCGGTGCCGACGTGGTACTTGGTGTGGAGATGACCA
  GTACTGGAGAGGTGGCCGGCTTCGGAGAGAGCCGTTGTGAAGCCTACCTCAAAGCCATGCTCAGCACTGG
  CTTTAAGATCCCAGAGAAGAACATCCTGCTGACTATTGGCAGCTACAAGAACAAAAGTGAGCTGCTCCCA
  ACTGTGCGGTTGCTGGAGAGCCTGGGCTACAGCCTCTATGCCAGCCTGGGTACGGCAGACTTCTACACTG
  AGCACGGGGTCAAGGTAACAGCTGTGGACTGGCACTTTGAAGAGGCGGTGGATGGTGAGTGCCCACCACA
  ACGGAGCATCTTGGATCAGCTGGCTGAAAATCACTTTGAGCTAGTGATTAACCTGTCCATGCGTGGGGCT
  GGGGGTCGGCGTCTTTCCTCCTTCGTTACCAAGGGCTACCGTACCCGGCGTCTGGCCGCTGATTTCTCTG
  TGCCTCTCATCATTGACATCAAGTGCACCAAACTCTTTGTGGAGGCCCTAGGCCAGATCGGCCCAGCCCC
  TCCTTTGAAAGTGCACGTTGACTGCATGACCTCCCAGAAGCTAGTGCGCCTGCCCGGATTGATTGATGTC
  CACGTGCACCTTCGGGAACCAGGTGGGACACACAAAGAGGACTTTGCCTCAGGCACAGCTGCTGCCTTGG
  CTGGGGGTGTCACCATGGTTTGTGCTATGCCTAATACCCGACCCCCCATCATTGATGCTCCTGCTCTCGC
  CCTTGCCCAGAAGCTGGCAGAGGCTGGCGCCCGCTGTGACTTTACCTTATTCCTTGGAGCCTCATCTGAG
  AACGCGGGGACTCTGGGTGCTGTGGCCGGCTCTGCAGCAGGCCTGAAGCTCTACCTCAACGAGACCTTCT
  CTGAGCTGCGGCTGGACAGTGTGGCCCAGTGGATGGAGCATTTTGAAACCTGGCCTGCCCACCTCCCCAT
  TGTGGCCCATGCAGAGCGGCAGAGTGTCGCTGCGGTCCTCATGGTGGCTCAGCTGACCCAGCGTCCAGTG
  CACATATGTCATGTGGCTCGGAAGGAAGAGATCCTGTTGATTAAAACTGCAAAGGCGCAGGGGCTGCCAG
  TGACCTGTGAGGTCGCTCCTCACCACCTGTTCCTAAACCGGGAAGACTTGGAGCGCCTGGGACCTGGGAA
  GGGAGAGGTCCGTCCTGAGCTTGGGTCCCGAGAGGATATGGAAGCCCTGTGGGAGAATATGGCAGTCATC
  GACTGTTTTGCCTCAGACCACGCTCCCCATACCTTGGAAGAGAAGTGTGGGCCCAAGCCTCCACCTGGCT
  TCCCGGGCCTAGAGACCATGTTGCCGCTGCTGCTGACAGCTGTGAGCGAGGGCCGCCTCAGTCTGGATGA
  CCTGCTGCAGCGATTGCACCACAACCCTCGGCGGATCTTCCACCTGCCTCTCCAGGAGGACACCTATGTA
  GAGGTGGATCTGGAGCATGAGTGGACAGTCCCCAGCCACATGCCCTTCTCTAAGGCCCGCTGGACCCCAT
  TTGAAGGACAGAAGGTGAAGGGCACAGTGCGCCGTGTGGTCTTGCGAGGGGAGGTCGCCTATATTGATGG
  ACAGGTGCTGGTACCCCCGGGCTATGGACAAGATGTACGGAAGTGGCCGCAGGGGGTTGTTCCCCAGCCC
  CCTCCTTCAACTCCCGCCACCACCGAAATCACCACGACACCTGAGCGACCACGCCGAGTCATCCCGGGCC
  TTCCTGATGGCCGCTTCCACCTGCCACCCCGCATCCACCGGGCCTCTGACCCAGGTCTGCCAGCTGAGGA
  ACCTAAAGAGAAGCCACCCAGGAAGGTAGTGGAACCAGAGCTGATGGGAACCCCTGATGGTCCCTGCTAC
  CCTGCACCACCAGTACCTAGACAGGCATCACCCCAGAACCTAGGGTCTTCTGGCCTGCTGCACCCCCAGA
  TGTCGCCCCTACTGCACTCCCTAGTGGGCCAGCACATCCTGTCTGTCAAGCAGTTCACTAAGGATCAGAT
  GTCTCATCTGTTCAATGTGGCGCACACGCTACGGATGATGGTACAGAAGGAGCGGAGCCTCGACATCCTG
  AAGCTGGCAGCCAAGCACTGTCGCAGGCCAGTGATCAATGCTGGGGATGGAGTTGGGGAGCACCCCACAC
  AGGCCCTGCTGGACATCTTCACCATTCGAGAAGAGCTGGGGACTGTCAATGGCATGACGATCACTATGGT
  CGGTGACCTGAAGCATGGACGCACAGTGCACTCCCTGGCCTGCCTGCTCACCCAGTACCGTGTGAGCCTA
  CGCTACGTGGCACCTCCCAGCCTGCGCATGCCACCCAGTGTACGGGACTTTGTGGCCTCCCGAGGCACCA
  AGCAGGAGGAGTTTGAGAGCATCGAGGAGGCGCTGCCCGACACAGACGTGCTCTACATGACTCGGATCCA
  GAAAGAACGATTTGGCTCTGTCCAGGAATATGAAGCTTGCTTTGGTCAGTTCATCCTCACTCCTCACATC
  ATGACCCGGGCCAAGAAGAAGATGGTGGTGATGCACCCAATGCCCCGTGTCAATGAGATAAGTGTGGAGG
  TGGACTCGGACCCCCGGGCAGCCTACTTCCGCCAAGCTGAGAACGGCATGTACATCCGCATGGCCCTGCT
  TGCCACCGTGCTTGGCCGTTTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289522
• Insert Size: 6465 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289522.1, NP_001276451.1
• RefSeq Size: 6924 bp
• RefSeq ORF: 6465 bp
• Locus ID: 69719
• UniProt ID: B2RQC6
• Cytogenetics: 5 B1
• Gene Summary: This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.