Slit2 (NM_001291228) Mouse Tagged ORF Clone

CAT#: MR231834

Slit2 (myc-DDK-tagged) - Mouse slit homolog 2 (Drosophila) (Slit2), transcript variant 2

Product Images

Circular map for MR231834

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slit2
• Synonyms: b2b1200.1Clo; Drad; Drad-1; E030015M03Rik; E130320P19Rik; mKIAA4141; S; Slil3; slit-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231834 representing NM_001291228
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGCATTGGCTGGCAGACACTGTCCCTATCGCTGGGGTTAGTGTTGTCGATCTTGAACAAGGTGG
  CGCCGCAGGCGTGCCCGGCCCAGTGCTCCTGTTCAGGCAGCACGGTGGACTGTCATGGGCTGGCACTGCG
  CAGTGTGCCCAGGAATATCCCCCGCAACACCGAGAGACTGGATTTGAATGGAAATAACATCACGAGGATC
  ACGAAGATAGATTTTGCTGGTCTCAGGCACCTCAGAGTTCTTCAGCTCATGGAGAACAGAATCAGCACCA
  TCGAGAGGGGAGCATTCCAGGATCTTAAGGAGCTGGAAAGACTGCGTTTAAACAGAAATAACCTTCAGTT
  GTTTCCTGAGCTGCTGTTTCTCGGGACTGCGAAGCTCTACCGGCTTGATCTCAGTGAAAATCAAATTCAA
  GCAATTCCAAGGAAGGCTTTCCGTGGGGCAGTTGACATTAAAAACCTGCAACTGGATTACAACCAGATCA
  GCTGCATTGAAGATGGGGCGTTCAGAGCTCTACGAGATCTGGAAGTGCTCACTCTGAACAATAACAATAT
  TACTAGACTTTCAGTGGCAAGTTTCAACCATATGCCTAAACTTAGGACATTTCGACTCCACTCGAACAAC
  TTGTACTGCGACTGCCACCTAGCCTGGCTCTCAGACTGGCTTCGCCAAAGGCCACGGGTGGGCTTGTACA
  CTCAGTGTATGGGCCCATCCCACCTGAGGGGCCACAATGTAGCAGAGGTTCAAAAACGAGAGTTTGTCTG
  CAGTGATGAGGAAGAAGGTCACCAGTCATTCATGGCTCCCTCCTGCAGTGTGCTGCACTGCCCCGCTGCT
  TGTACCTGTAGCAACAACATTGTAGACTGCCGAGGGAAAGGTCTCACTGAGATCCCCACAAATCTGCCTG
  AGACCATCACAGAAATACGTTTGGAACAGAACTCCATCAGGGTCATCCCTCCAGGAGCCTTCTCACCATA
  CAAAAAGCTTAGACGACTAGACCTGAGCAACAACCAGATCTCTGAACTTGCACCAGATGCCTTCCAAGGA
  CTGCGCTCTCTGAATTCACTTGTCCTGTATGGAAATAAAATCACAGAACTCCCAAAAAGTTTATTCGAAG
  GACTATTTTCCTTGCAGCTACTATTATTGAATGCCAACAAGATAAACTGCCTTCGGGTAGATGCTTTTCA
  GGACCTGCACAACTTGAACCTTCTCTCCTTATATGACAATAAGCTTCAGACGGTTGCCAAGGGCACCTTC
  TCAGCCCTCAGAGCCATCCAAACTATGCATTTGGCCCAGAATCCTTTCATTTGTGACTGCCATCTCAAGT
  GGCTAGCGGATTATCTCCACACCAACCCAATTGAGACCAGCGGTGCCCGTTGCACCAGCCCCCGCCGCCT
  GGCAAACAAAAGAATTGGACAGATCAAAAGCAAGAAATTCCGTTGTTCAGGTACAGAAGATTATCGATCA
  AAATTAAGTGGAGACTGCTTTGCAGACTTGGCTTGTCCTGAGAAGTGTCGCTGTGAAGGGACCACAGTAG
  ACTGCTCCAATCAAAGACTCAACAAAATCCCTGACCATATTCCCCAGTACACAGCAGAGCTGCGTCTCAA
  TAATAATGAATTCACAGTGTTAGAAGCCACGGGAATATTTAAGAAACTTCCTCAGTTACGTAAAATCAAC
  TTTAGCAACAATAAGATCACGGATATCGAGGAGGGTGCATTTGAAGGCGCGTCTGGTGTGAATGAAATTC
  TTCTCACCAGTAACCGTTTGGAAAATGTTCAGCATAAGATGTTCAAAGGACTGGAGAGCCTCAAAACATT
  GATGCTGAGAAGTAATCGAATAAGCTGTGTTGGGAACGACAGTTTCATAGGACTCGGCTCTGTGCGTCTG
  CTCTCTTTATATGACAATCAAATTACCACAGTGGCACCAGGAGCATTTGATTCTCTCCATTCATTATCCA
  CTCTAAACCTCTTGGCCAATCCTTTCAACTGTAACTGTCACCTGGCATGGCTGGGAGAATGGCTCAGAAG
  GAAAAGAATTGTAACAGGAAATCCTCGATGCCAAAAACCCTACTTCCTGAAGGAAATCCCAATCCAGGAT
  GTAGCCATTCAGGACTTCACCTGTGATGATGGAAATGATGACAATAGTTGCTCTCCACTCTCCCGTTGTC
  CTTCTGAATGTACCTGCTTGGATACAGTGGTACGATGTAGCAACAAGGGCTTGAAGGTTTTGCCTAAAGG
  TATTCCAAAAGATGTCACAGAGCTGTATCTGGATGGGAACCAGTTTACGCTGGTCCCGAAGGAACTCTCT
  AACTACAAACATTTAACACTTATAGACTTAAGTAACAACCGAATAAGCACCCTTTCCAATCAAAGCTTCA
  GCAACATGACCCAGCTTCTCACCTTAATCCTCAGTTACAACCGTCTGAGATGTATCCCTCCACGAACCTT
  TGATGGATTGAAGTCTCTTCGGTTACTGTCTTTACATGGAAATGACATTTCTGTTGTGCCTGAAGGTGCC
  TTCAATGACTTGTCAGCCTTGTCACACTTAGCGATTGGAGCCAACCCTCTTTACTGTGATTGTAACATGC
  AGTGGTTATCCGACTGGGTGAAGTCGGAATATAAGGAACCTGGAATTGCACGCTGTGCCGGCCCTGGAGA
  AATGGCAGATAAATTATTACTCACTACTCCCTCCAAAAAATTTACATGTCAAGGTCCCGTGGATATCACT
  ATTCAAGCCAAGTGTAATCCCTGCTTATCAAATCCATGTAAAAATGATGGCACCTGTAACAATGACCCCG
  TTGATTTTTATCGATGTACCTGCCCATATGGATTCAAGGGTCAGGACTGTGATGTCCCCATTCATGCTTG
  TATCAGTAATCCATGTAAACATGGAGGAACTTGTCACTTAAAGGAAGGAGAGAATGCTGGATTCTGGTGC
  ACTTGTGCTGATGGGTTTGAAGGAGAAAACTGTGAAGTCAATATTGATGATTGTGAAGATAATGATTGTG
  AAAATAATTCTACATGCGTTGATGGAATTAACAACTACACATGTCTTTGCCCACCGGAATACACAGGTGA
  ACTGTGTGAGGAAAAGCTGGACTTCTGTGCACAAGACTTGAATCCCTGCCAGCATGACTCCAAGTGCATC
  CTGACTCCAAAGGGATTCAAGTGTGACTGCACTCCAGGATACATTGGTGAGCACTGTGACATTGACTTTG
  ATGACTGCCAAGATAACAAGTGTAAAAACGGTGCTCACTGCACAGATGCCGTGAACGGATACACGTGCGT
  CTGTCCTGAAGGCTACAGTGGCTTGTTCTGTGAGTTTTCTCCACCCATGGTCCTCCCTCGCACCAGCCCC
  TGTGATAATTTTGATTGCCAGAATGGAGCCCAGTGTATCATCAGGATAAATGAACCAATATGCCAGTGTT
  TGCCTGGCTACCTGGGAGAGAAGTGTGAGAAATTGGTCAGTGTGAATTTTGTAAACAAAGAGTCCTATCT
  TCAGATTCCTTCAGCCAAGGTTCGGCCTCAGACAAACATCACACTTCAGATTGCCACAGATGAAGACAGC
  GGCATCCTCTTGTATAAAGGTGACAAAGACCACATTGCCGTGGAACTCTATAGAGGGCGAGTTCGAGCCA
  GCTATGACACCGGCTCTCATCCGGCTTCTGCCATTTACAGTGTGGAGACAATCAATGATGGAAACTTCCA
  CATTGTGGAGCTACTGACCCTGGATTCCAGTCTTTCCCTCTCTGTGGATGGAGGAAGCCCTAAAGTCATC
  ACCAATTTGTCAAAACAATCTACTCTGAATTTCGACTCTCCACTCTATGTAGGAGGCATGCCTGGGAAAA
  ATAACGTGGCATCCCTGCGCCAGGCCCCTGGGCAAAATGGCACCAGCTTCCATGGCTGTATCCGGAACCT
  TTACATTAACAGTGAGCTGCAGGACTTCCGGAAAATGCCTATGCAAACCGGAATTCTGCCTGGCTGTGAA
  CCATGCCACAAGAAAGTATGTGCCCATGGCATGTGCCAGCCCAGCAGCCAATCAGGCTTCACCTGTGAAT
  GTGAGGAAGGGTGGATGGGGCCCCTCTGTGACCAGAGAACCAATGATCCCTGCCTCGGAAACAAATGTGT
  GCATGGGACCTGCCTGCCCATCAATGCCTTCTCCTATAGTTGCAAGTGCCTGGAGGGCCATGGCGGTGTC
  CTCTGTGATGAAGAAGAAGATCTCTTTAACCCCTGCCAGATGATCAAGTGCAAGCATGGGAAGTGCAGGC
  TTTCTGGAGTGGGCCAGCCCTATTGTGAATGCAACAGTGGATTCACCGGGGACAGCTGTGATAGAGAAAT
  TTCTTGTCGAGGGGAACGGATAAGGGACTATTACCAGAAGCAGCAGGGTTACGCTGCCTGTCAAACAACT
  AAGAAAGTATCTCGCTTGGAATGCAGAGGCGGGTGCGCTGGAGGCCAGTGCTGTGGACCTCTGAGAAGCA
  AGAGGCGGAAATACTCTTTCGAATGCACAGATGGCTCCTCATTTGTGGACGAGGTTGAGAAAGTGGTGAA
  GTGCGGCTGCGCGAGATGTGCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231834 representing NM_001291228
  Red=Cloning site Green=Tags(s)
  
  MSGIGWQTLSLSLGLVLSILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRI
  TKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQ
  AIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNN
  LYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAA
  CTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQG
  LRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTF
  SALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRS
  KLSGDCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKIN
  FSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRL
  LSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGNPRCQKPYFLKEIPIQD
  VAIQDFTCDDGNDDNSCSPLSRCPSECTCLDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQFTLVPKELS
  NYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGA
  FNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDIT
  IQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAGFWC
  TCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCI
  LTPKGFKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSPPMVLPRTSP
  CDNFDCQNGAQCIIRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDS
  GILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVI
  TNLSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRKMPMQTGILPGCE
  PCHKKVCAHGMCQPSSQSGFTCECEEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGV
  LCDEEEDLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDREISCRGERIRDYYQKQQGYAACQTT
  KKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCARCAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291228
• ORF Size: 4575 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291228.2, NP_001278157.1
• RefSeq Size: 8504 bp
• RefSeq ORF: 4578 bp
• Locus ID: 20563
• Cytogenetics: 5 B3
• MW: 169.7 kDa
• Gene Summary: The protein encoded by this gene is a member of the Slit family of secreted glycoproteins, which function as ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. In mammals, members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Mice deficient for this gene exhibit abnormal axonal projections in the embryonic forebrain and develop supernumerary uretic buds that maintain improper connections to the nephric duct. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]