Slit2 (NM_001291228) Mouse Untagged Clone

CAT#: MC229623

Slit2 (untagged) - Mouse slit homolog 2 (Drosophila) (Slit2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slit2
• Synonyms: b2b1200.1Clo; Drad; Drad-1; E030015M03Rik; E130320P19Rik; mKIAA4141; S; Slil3; slit-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229623 representing NM_001291228
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGCATTGGCTGGCAGACACTGTCCCTATCGCTGGGGTTAGTGTTGTCGATCTTGAACAAGGTGG
  CGCCGCAGGCGTGCCCGGCCCAGTGCTCCTGTTCAGGCAGCACGGTGGACTGTCATGGGCTGGCACTGCG
  CAGTGTGCCCAGGAATATCCCCCGCAACACCGAGAGACTGGATTTGAATGGAAATAACATCACGAGGATC
  ACGAAGATAGATTTTGCTGGTCTCAGGCACCTCAGAGTTCTTCAGCTCATGGAGAACAGAATCAGCACCA
  TCGAGAGGGGAGCATTCCAGGATCTTAAGGAGCTGGAAAGACTGCGTTTAAACAGAAATAACCTTCAGTT
  GTTTCCTGAGCTGCTGTTTCTCGGGACTGCGAAGCTCTACCGGCTTGATCTCAGTGAAAATCAAATTCAA
  GCAATTCCAAGGAAGGCTTTCCGTGGGGCAGTTGACATTAAAAACCTGCAACTGGATTACAACCAGATCA
  GCTGCATTGAAGATGGGGCGTTCAGAGCTCTACGAGATCTGGAAGTGCTCACTCTGAACAATAACAATAT
  TACTAGACTTTCAGTGGCAAGTTTCAACCATATGCCTAAACTTAGGACATTTCGACTCCACTCGAACAAC
  TTGTACTGCGACTGCCACCTAGCCTGGCTCTCAGACTGGCTTCGCCAAAGGCCACGGGTGGGCTTGTACA
  CTCAGTGTATGGGCCCATCCCACCTGAGGGGCCACAATGTAGCAGAGGTTCAAAAACGAGAGTTTGTCTG
  CAGTGATGAGGAAGAAGGTCACCAGTCATTCATGGCTCCCTCCTGCAGTGTGCTGCACTGCCCCGCTGCT
  TGTACCTGTAGCAACAACATTGTAGACTGCCGAGGGAAAGGTCTCACTGAGATCCCCACAAATCTGCCTG
  AGACCATCACAGAAATACGTTTGGAACAGAACTCCATCAGGGTCATCCCTCCAGGAGCCTTCTCACCATA
  CAAAAAGCTTAGACGACTAGACCTGAGCAACAACCAGATCTCTGAACTTGCACCAGATGCCTTCCAAGGA
  CTGCGCTCTCTGAATTCACTTGTCCTGTATGGAAATAAAATCACAGAACTCCCAAAAAGTTTATTCGAAG
  GACTATTTTCCTTGCAGCTACTATTATTGAATGCCAACAAGATAAACTGCCTTCGGGTAGATGCTTTTCA
  GGACCTGCACAACTTGAACCTTCTCTCCTTATATGACAATAAGCTTCAGACGGTTGCCAAGGGCACCTTC
  TCAGCCCTCAGAGCCATCCAAACTATGCATTTGGCCCAGAATCCTTTCATTTGTGACTGCCATCTCAAGT
  GGCTAGCGGATTATCTCCACACCAACCCAATTGAGACCAGCGGTGCCCGTTGCACCAGCCCCCGCCGCCT
  GGCAAACAAAAGAATTGGACAGATCAAAAGCAAGAAATTCCGTTGTTCAGGTACAGAAGATTATCGATCA
  AAATTAAGTGGAGACTGCTTTGCAGACTTGGCTTGTCCTGAGAAGTGTCGCTGTGAAGGGACCACAGTAG
  ACTGCTCCAATCAAAGACTCAACAAAATCCCTGACCATATTCCCCAGTACACAGCAGAGCTGCGTCTCAA
  TAATAATGAATTCACAGTGTTAGAAGCCACGGGAATATTTAAGAAACTTCCTCAGTTACGTAAAATCAAC
  TTTAGCAACAATAAGATCACGGATATCGAGGAGGGTGCATTTGAAGGCGCGTCTGGTGTGAATGAAATTC
  TTCTCACCAGTAACCGTTTGGAAAATGTTCAGCATAAGATGTTCAAAGGACTGGAGAGCCTCAAAACATT
  GATGCTGAGAAGTAATCGAATAAGCTGTGTTGGGAACGACAGTTTCATAGGACTCGGCTCTGTGCGTCTG
  CTCTCTTTATATGACAATCAAATTACCACAGTGGCACCAGGAGCATTTGATTCTCTCCATTCATTATCCA
  CTCTAAACCTCTTGGCCAATCCTTTCAACTGTAACTGTCACCTGGCATGGCTGGGAGAATGGCTCAGAAG
  GAAAAGAATTGTAACAGGAAATCCTCGATGCCAAAAACCCTACTTCCTGAAGGAAATCCCAATCCAGGAT
  GTAGCCATTCAGGACTTCACCTGTGATGATGGAAATGATGACAATAGTTGCTCTCCACTCTCCCGTTGTC
  CTTCTGAATGTACCTGCTTGGATACAGTGGTACGATGTAGCAACAAGGGCTTGAAGGTTTTGCCTAAAGG
  TATTCCAAAAGATGTCACAGAGCTGTATCTGGATGGGAACCAGTTTACGCTGGTCCCGAAGGAACTCTCT
  AACTACAAACATTTAACACTTATAGACTTAAGTAACAACCGAATAAGCACCCTTTCCAATCAAAGCTTCA
  GCAACATGACCCAGCTTCTCACCTTAATCCTCAGTTACAACCGTCTGAGATGTATCCCTCCACGAACCTT
  TGATGGATTGAAGTCTCTTCGGTTACTGTCTTTACATGGAAATGACATTTCTGTTGTGCCTGAAGGTGCC
  TTCAATGACTTGTCAGCCTTGTCACACTTAGCGATTGGAGCCAACCCTCTTTACTGTGATTGTAACATGC
  AGTGGTTATCCGACTGGGTGAAGTCGGAATATAAGGAACCTGGAATTGCACGCTGTGCCGGCCCTGGAGA
  AATGGCAGATAAATTATTACTCACTACTCCCTCCAAAAAATTTACATGTCAAGGTCCCGTGGATATCACT
  ATTCAAGCCAAGTGTAATCCCTGCTTATCAAATCCATGTAAAAATGATGGCACCTGTAACAATGACCCCG
  TTGATTTTTATCGATGTACCTGCCCATATGGATTCAAGGGTCAGGACTGTGATGTCCCCATTCATGCTTG
  TATCAGTAATCCATGTAAACATGGAGGAACTTGTCACTTAAAGGAAGGAGAGAATGCTGGATTCTGGTGC
  ACTTGTGCTGATGGGTTTGAAGGAGAAAACTGTGAAGTCAATATTGATGATTGTGAAGATAATGATTGTG
  AAAATAATTCTACATGCGTTGATGGAATTAACAACTACACATGTCTTTGCCCACCGGAATACACAGGTGA
  ACTGTGTGAGGAAAAGCTGGACTTCTGTGCACAAGACTTGAATCCCTGCCAGCATGACTCCAAGTGCATC
  CTGACTCCAAAGGGATTCAAGTGTGACTGCACTCCAGGATACATTGGTGAGCACTGTGACATTGACTTTG
  ATGACTGCCAAGATAACAAGTGTAAAAACGGTGCTCACTGCACAGATGCCGTGAACGGATACACGTGCGT
  CTGTCCTGAAGGCTACAGTGGCTTGTTCTGTGAGTTTTCTCCACCCATGGTCCTCCCTCGCACCAGCCCC
  TGTGATAATTTTGATTGCCAGAATGGAGCCCAGTGTATCATCAGGATAAATGAACCAATATGCCAGTGTT
  TGCCTGGCTACCTGGGAGAGAAGTGTGAGAAATTGGTCAGTGTGAATTTTGTAAACAAAGAGTCCTATCT
  TCAGATTCCTTCAGCCAAGGTTCGGCCTCAGACAAACATCACACTTCAGATTGCCACAGATGAAGACAGC
  GGCATCCTCTTGTATAAAGGTGACAAAGACCACATTGCCGTGGAACTCTATAGAGGGCGAGTTCGAGCCA
  GCTATGACACCGGCTCTCATCCGGCTTCTGCCATTTACAGTGTGGAGACAATCAATGATGGAAACTTCCA
  CATTGTGGAGCTACTGACCCTGGATTCCAGTCTTTCCCTCTCTGTGGATGGAGGAAGCCCTAAAGTCATC
  ACCAATTTGTCAAAACAATCTACTCTGAATTTCGACTCTCCACTCTATGTAGGAGGCATGCCTGGGAAAA
  ATAACGTGGCATCCCTGCGCCAGGCCCCTGGGCAAAATGGCACCAGCTTCCATGGCTGTATCCGGAACCT
  TTACATTAACAGTGAGCTGCAGGACTTCCGGAAAATGCCTATGCAAACCGGAATTCTGCCTGGCTGTGAA
  CCATGCCACAAGAAAGTATGTGCCCATGGCATGTGCCAGCCCAGCAGCCAATCAGGCTTCACCTGTGAAT
  GTGAGGAAGGGTGGATGGGGCCCCTCTGTGACCAGAGAACCAATGATCCCTGCCTCGGAAACAAATGTGT
  GCATGGGACCTGCCTGCCCATCAATGCCTTCTCCTATAGTTGCAAGTGCCTGGAGGGCCATGGCGGTGTC
  CTCTGTGATGAAGAAGAAGATCTCTTTAACCCCTGCCAGATGATCAAGTGCAAGCATGGGAAGTGCAGGC
  TTTCTGGAGTGGGCCAGCCCTATTGTGAATGCAACAGTGGATTCACCGGGGACAGCTGTGATAGAGAAAT
  TTCTTGTCGAGGGGAACGGATAAGGGACTATTACCAGAAGCAGCAGGGTTACGCTGCCTGTCAAACAACT
  AAGAAAGTATCTCGCTTGGAATGCAGAGGCGGGTGCGCTGGAGGCCAGTGCTGTGGACCTCTGAGAAGCA
  AGAGGCGGAAATACTCTTTCGAATGCACAGATGGCTCCTCATTTGTGGACGAGGTTGAGAAAGTGGTGAA
  GTGCGGCTGCGCGAGATGTGCCTCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291228
• Insert Size: 4578 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291228.2, NP_001278157.1
• RefSeq Size: 9970 bp
• RefSeq ORF: 4578 bp
• Locus ID: 20563
• Cytogenetics: 5 B3
• Gene Summary: The protein encoded by this gene is a member of the Slit family of secreted glycoproteins, which function as ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. In mammals, members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Mice deficient for this gene exhibit abnormal axonal projections in the embryonic forebrain and develop supernumerary uretic buds that maintain improper connections to the nephric duct. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (2) lacks alternate in-frame exons in the 5' and central coding regions compared to variant 1. It encodes isoform b, which is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.