Clasp1 (NM_001293301) Mouse Tagged ORF Clone

CAT#: MR231805

Clasp1 (myc-DDK-tagged) - Mouse CLIP associating protein 1 (Clasp1), transcript variant 5

Product Images

Circular map for MR231805

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Clasp1
• Synonyms: 1700030C23Rik; 5730583A19Rik; B130045P17Rik; CLASP1alpha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231805 representing NM_001293301
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCGAGAATGGAGTCCTGTCTGGCCCAGGTGCTGCAGAAGGATGTGGGAAAGCGGCTGCAGGTTG
  GCCAGGAACTCATAGACTATTTCTCAGACAGACAGAAGTCTGCTGACCTTGAGCACGACCAGACCCTGTT
  GGATAAGCTTGTGGATGGACTCGCTACCTCTTGGGTGAACTCTAGCAATTACAAGGTGGTTCTCTTGGGC
  ATGGACATCCTGTCGGCACTGGTGACTAGGCTGCAGGACCGGTTCAAGGCGCAAATCGGCACAGTGTTGC
  CAAGTCTAATAGACAGACTGGGAGATGCTAAAGACTCCGTGAGGGAGCAAGACCAAACCCTGCTGCTAAA
  GATCATGGATCAAGCTGCTAATCCCCAGTATGTGTGGGACAGAATGCTCGGAGGCTTCAAACACAAGAAC
  TTCCGCACAAGAGAGGGCATCTGCCTCTGCCTTATTGCAACACTCAATGCCTCTGGGGCCCAGACTCTAA
  CACTAAGCAAGATTGTGCCACATATATGTAACCTACTGGGAGATCCCAACAGCCAGGTTCGAGACGCAGC
  AATAAACAGTCTGGTGGAGATTTATAGACATGTAGGTGAACGTGTGAGGGCAGACCTCAGTAAGAAAGGA
  CTGCCACAGTCCCGGTTGAATGTCATTTTTACAAAATTTGATGAAGTCCAAAAGTCTGGAAATATGATAC
  AGTCTGCAAATGAAAAAAATTTTGATGATGAAGATTCTGTGGATGGCAACAGGCCTTCTTCTGCCAGCTC
  CTCATCATCCAAGGCCCCATCAAGTTCCCGGAGGAATGTTAACCTGGGGACCACCCGTAGGCTCATGTCA
  TCCAGTCTTGGATCTAAGTCTTCAGCTGCAAAAGAAGGCGCTGGTGCTGTGGATGAAGAGGATTTTATTA
  AAGCCTTTGATGATGTACCTGTAGTGCAGATTTACTCCAGCCGAGACCTCGAGGAATCCATAAACAAAAT
  CAGAGAAATCCTGTCAGATGACAAGCATGACTGGGAGCAGAGAGTAAATGCTCTAAAAAAGATTAGATCG
  TTACTCTTGGCTGGGGCTGCTGAGTACGATAACTTCTTTCAACACTTGCGTCTTCTGGACGGGGCCTTTA
  AACTCTCTGCTAAGGACCTGCGGTCTCAGGTAGTGCGGGAAGCTTGTATCACATTGGGGCATCTGTCATC
  AGTTCTGGGAAATAAGTTTGATCATGGAGCTGAAGCCATTATGCCAACTATCTTTAATTTAATCCCGAAC
  AGTGCCAAAATTATGGCTACTTCTGGTGTTGTAGCTGTTAGGCTAATCATTCGGCACACTCACATCCCTC
  GGCTGATCCCTGTCATAACCAGCAACTGTACCTCTAAGTCTGTCGCCGTCAGAAGGCGCTGTTTTGAATT
  TTTAGATTTGCTTTTACAAGAATGGCAGACACATTCACTAGAAAGACATATATCAGTATTAGCTGAAACA
  ATAAAGAAAGGAATACACGACGCTGATTCTGAAGCGAGAATAGAAGCCAGGAAGTGCTACTGGGGTTTCC
  ACAGTCACTTCAGCCGAGAAGCAGAACACCTGTACCACACTTTGGAGTCCTCGTATCAGAAGGCCCTACA
  GTCCCACTTGAAGAACTCGGACAGCATCGTGTCTCTGCCCCAGTCAGACCGATCCTCTTCCAGCTCTCAA
  GAGAGTCTCAACCGGCCACTTTCAGCCAAAAGAAGTCCCACTGGCAGCACTGCATCCAGAGGCTCTACAG
  TTAGTACCAAATCTGTGTCGACGACAGGATCTCTCCAGCGATCTCGAAGTGATATTGATGTGAACGCAGC
  AGCCAGTGCCAAATCCAAAGTCTCCTCATCCTCAGGATCCCCCGCCTTCAGCTCTGCAGCAGCACTGCCC
  CCAGGATCCTATGCGTCCCTAGGTCGGATCCGCACGAGACGGCAGAGCTCGGGGAGCACCACCAATGTCG
  CCTCCACACCCTCCGACAGTCGGGGCCGCAGTCGCGCCAAAGTGGTTTCACAGTCTCAGCCTGGCAGCCG
  GTCAAGTTCCCCTGGGAAGCTTTTGGGAAGTGGACTTGCTGGGGGTTCCTCCAGAGGCCCACCGGTAACA
  CCATCCTCAGAAAAACGGAGCAAGATCCCCAGGAGTCAGGGATGTAGCCGAGAAACAAGTCCTAACCGGA
  TTGGATTAGATCGGTTTGGGCTGGGCCAGTCAGGACGAATCCCTGGTTCTGTGAACGCCATGAGAGTCTT
  GAGTACCAGCACTGACCTGGAAGCAGCAGTGGCTGACGCTCTGAAGAAGCCTGTGAGAAGGAGATACGAG
  CCCTATGGAATGTACTCTGATGATGATGCCAACAGTGATGCCTCCAGCGTGTGCTCTGAGCGCTCATATG
  GCTCCAGGAATGGTGGCATTCCCCATTATCTGCGGCAGACTGAAGATGTAGCAGAGGTTCTCAACCATTG
  TGCCAGTTCCAACTGGTCAGAACGGAAAGAGGGGCTCCTGGGCCTGCAGAACTTACTGAAGAGCCAAAGA
  ACACTGAGTCGAGTAGAATTGAAGAGACTGTGTGAGATTTTCACCCGAATGTTTGCCGACCCTCACAGCA
  AGAGAGTTTTCAGTATGTTTTTGGAGACCCTCGTGGATTTTATAATCATTCATAAGGATGACTTGCAAGA
  CTGGCTCTTCGTCCTTCTCACACAACTACTTAAGAAAATGGGAGCAGACTTACTTGGATCTGTGCAAGCA
  AAAGTTCAGAAGGCTCTCGATGTCACCAGGGATTCCTTTCCATTTGATCAACAATTTAACATTTTGATGA
  GATTTATTGTGGATCAGACTCAAACTCCAAACCTCAAGGTCAAAGTAGCAATCCTGAAATACATCGAATC
  TCTAGCCAGACAGATGGACCCCACAGATTTTGTAAATTCCAGCGAAACAAGACTAGCTGTTTCTAGGATC
  ATAACATGGACTACAGAACCAAAGAGTTCAGACGTGAGAAAGGCAGCACAAATTGTGCTCATCTCTCTGT
  TTGAACTGAACACGCCTGAATTTACCATGTTACTTGGTGCCTTGCCAAAAACATTCCAGGATGGTGCCAC
  CAAACTCCTGCATAACCACCTCAAGAACTCCAGTAACACCGGTGTGGGATCTCCAAGCAATACAATTGGC
  CGGACACCTTCCCGCCACCCCAGCAGCAGGACCAGCCCCCTGACCTCACCCACCAACTGTTCCCATGGGG
  GACTATCTCCAAGCATGCTGGACTATGATACAGAGAACCTGAACTCTGAAGAAATCTACAGCTCTTTGCG
  TGGAGTTACAGAAGCCATTGAAAAGTTCAGCTTCCGAAGCCAGGAGGATCTAAATGAGCCAATCAAACGA
  GATGGCAAGAAGGATTGTGATATCGTGTCCCGAGATGGGGGAGCAGCCTCACCTGCCACCGAGGGCCGGG
  GAGGTAGTGAGATAGAAGGAGGCAGGATGGCTTTGGACAACAAGACCTCCCTGCTCAACACGCAGCCTCC
  ACGTGCCTTTCCGGGGCCAAGAGCACGGGAATATAACCCGTATCCCTACTCCGACACCATCAACACCTAT
  GACAAGACGGCTCTGAAGGAAGCAGTGTTTGACGATGACATGGAGCAGCTCCGAGATGTGCCCATTGACC
  ACTCAGACCTGGTGGCTGACTTGCTGAAAGAGCTATCTAACCACAACGAGCGTGTGGAGGAGCGGAAGGG
  CGCACTGCTGGAGTTGCTCAAGATCACCAGGGAGGACAGCCTGGGCGTGTGGGAGGAGCACTTCAAGACC
  ATCCTGCTGCTGCTGCTGGAAACTCTCGGAGACAAAGACCATTCCATTCGAGCTCTGGCACTGAGAGTTT
  TACGGGAAATTCTGAGAAACCAGCCAGCAAGATTCAAAAACTATGCAGAACTGACGATCATGAAGACTCT
  GGAAGCCCACAAAGACTCCCACAAGGAGGTGGTGAGAGCGGCCGAGGAAGCTGCATCCACGCTAGCCAGC
  TCAATCCACCCAGAGCAGTGCATCAAAGTGCTGTGTCCAATCATCCAGACAGCCGACTACCCCATCAACC
  TGGCTGCTATCAAGATGCAGACAAAGGTGGTGGAGAGGATCACCAAGGAGTCCTTGCTGCAGCTCCTCGT
  CGACATCATCCCCGGCCTGCTGCAGGGTTACGACAACACCGAGAGCAGTGTACGGAAAGCCAGTGTGTTT
  TGCTTAGTGGCAATCTATTCCGTAATCGGAGAAGATCTGAAACCTCACCTCGCACAGCTCACGGGGAGCA
  AGATGAAGCTGCTGAACTTATATATAAAGAGGGCCCAGACTACCAACAGCAACAGCAGCTCTTCCTCTGA
  TGTGTCCACACACAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231805 representing NM_001293301
  Red=Cloning site Green=Tags(s)
  
  MEPRMESCLAQVLQKDVGKRLQVGQELIDYFSDRQKSADLEHDQTLLDKLVDGLATSWVNSSNYKVVLLG
  MDILSALVTRLQDRFKAQIGTVLPSLIDRLGDAKDSVREQDQTLLLKIMDQAANPQYVWDRMLGGFKHKN
  FRTREGICLCLIATLNASGAQTLTLSKIVPHICNLLGDPNSQVRDAAINSLVEIYRHVGERVRADLSKKG
  LPQSRLNVIFTKFDEVQKSGNMIQSANEKNFDDEDSVDGNRPSSASSSSSKAPSSSRRNVNLGTTRRLMS
  SSLGSKSSAAKEGAGAVDEEDFIKAFDDVPVVQIYSSRDLEESINKIREILSDDKHDWEQRVNALKKIRS
  LLLAGAAEYDNFFQHLRLLDGAFKLSAKDLRSQVVREACITLGHLSSVLGNKFDHGAEAIMPTIFNLIPN
  SAKIMATSGVVAVRLIIRHTHIPRLIPVITSNCTSKSVAVRRRCFEFLDLLLQEWQTHSLERHISVLAET
  IKKGIHDADSEARIEARKCYWGFHSHFSREAEHLYHTLESSYQKALQSHLKNSDSIVSLPQSDRSSSSSQ
  ESLNRPLSAKRSPTGSTASRGSTVSTKSVSTTGSLQRSRSDIDVNAAASAKSKVSSSSGSPAFSSAAALP
  PGSYASLGRIRTRRQSSGSTTNVASTPSDSRGRSRAKVVSQSQPGSRSSSPGKLLGSGLAGGSSRGPPVT
  PSSEKRSKIPRSQGCSRETSPNRIGLDRFGLGQSGRIPGSVNAMRVLSTSTDLEAAVADALKKPVRRRYE
  PYGMYSDDDANSDASSVCSERSYGSRNGGIPHYLRQTEDVAEVLNHCASSNWSERKEGLLGLQNLLKSQR
  TLSRVELKRLCEIFTRMFADPHSKRVFSMFLETLVDFIIIHKDDLQDWLFVLLTQLLKKMGADLLGSVQA
  KVQKALDVTRDSFPFDQQFNILMRFIVDQTQTPNLKVKVAILKYIESLARQMDPTDFVNSSETRLAVSRI
  ITWTTEPKSSDVRKAAQIVLISLFELNTPEFTMLLGALPKTFQDGATKLLHNHLKNSSNTGVGSPSNTIG
  RTPSRHPSSRTSPLTSPTNCSHGGLSPSMLDYDTENLNSEEIYSSLRGVTEAIEKFSFRSQEDLNEPIKR
  DGKKDCDIVSRDGGAASPATEGRGGSEIEGGRMALDNKTSLLNTQPPRAFPGPRAREYNPYPYSDTINTY
  DKTALKEAVFDDDMEQLRDVPIDHSDLVADLLKELSNHNERVEERKGALLELLKITREDSLGVWEEHFKT
  ILLLLLETLGDKDHSIRALALRVLREILRNQPARFKNYAELTIMKTLEAHKDSHKEVVRAAEEAASTLAS
  SIHPEQCIKVLCPIIQTADYPINLAAIKMQTKVVERITKESLLQLLVDIIPGLLQGYDNTESSVRKASVF
  CLVAIYSVIGEDLKPHLAQLTGSKMKLLNLYIKRAQTTNSNSSSSSDVSTHS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001293301
• ORF Size: 4356 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293301.1, NP_001280230.1
• RefSeq Size: 7651 bp
• RefSeq ORF: 4359 bp
• Locus ID: 76707
• UniProt ID: Q80TV8
• Cytogenetics: 1 E2.3
• MW: 160.7 kDa
• Gene Summary: Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (By similarity).[UniProtKB/Swiss-Prot Function]