Clasp1 (NM_001293301) Mouse Untagged Clone

CAT#: MC229594

Clasp1 (untagged) - Mouse CLIP associating protein 1 (Clasp1), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Clasp1
• Synonyms: 1700030C23Rik; 5730583A19Rik; B130045P17Rik; CLASP1alpha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229594 representing NM_001293301
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCGAGAATGGAGTCCTGTCTGGCCCAGGTGCTGCAGAAGGATGTGGGAAAGCGGCTGCAGGTTG
  GCCAGGAACTCATAGACTATTTCTCAGACAGACAGAAGTCTGCTGACCTTGAGCACGACCAGACCCTGTT
  GGATAAGCTTGTGGATGGACTCGCTACCTCTTGGGTGAACTCTAGCAATTACAAGGTGGTTCTCTTGGGC
  ATGGACATCCTGTCGGCACTGGTGACTAGGCTGCAGGACCGGTTCAAGGCGCAAATCGGCACAGTGTTGC
  CAAGTCTAATAGACAGACTGGGAGATGCTAAAGACTCCGTGAGGGAGCAAGACCAAACCCTGCTGCTAAA
  GATCATGGATCAAGCTGCTAATCCCCAGTATGTGTGGGACAGAATGCTCGGAGGCTTCAAACACAAGAAC
  TTCCGCACAAGAGAGGGCATCTGCCTCTGCCTTATTGCAACACTCAATGCCTCTGGGGCCCAGACTCTAA
  CACTAAGCAAGATTGTGCCACATATATGTAACCTACTGGGAGATCCCAACAGCCAGGTTCGAGACGCAGC
  AATAAACAGTCTGGTGGAGATTTATAGACATGTAGGTGAACGTGTGAGGGCAGACCTCAGTAAGAAAGGA
  CTGCCACAGTCCCGGTTGAATGTCATTTTTACAAAATTTGATGAAGTCCAAAAGTCTGGAAATATGATAC
  AGTCTGCAAATGAAAAAAATTTTGATGATGAAGATTCTGTGGATGGCAACAGGCCTTCTTCTGCCAGCTC
  CTCATCATCCAAGGCCCCATCAAGTTCCCGGAGGAATGTTAACCTGGGGACCACCCGTAGGCTCATGTCA
  TCCAGTCTTGGATCTAAGTCTTCAGCTGCAAAAGAAGGCGCTGGTGCTGTGGATGAAGAGGATTTTATTA
  AAGCCTTTGATGATGTACCTGTAGTGCAGATTTACTCCAGCCGAGACCTCGAGGAATCCATAAACAAAAT
  CAGAGAAATCCTGTCAGATGACAAGCATGACTGGGAGCAGAGAGTAAATGCTCTAAAAAAGATTAGATCG
  TTACTCTTGGCTGGGGCTGCTGAGTACGATAACTTCTTTCAACACTTGCGTCTTCTGGACGGGGCCTTTA
  AACTCTCTGCTAAGGACCTGCGGTCTCAGGTAGTGCGGGAAGCTTGTATCACATTGGGGCATCTGTCATC
  AGTTCTGGGAAATAAGTTTGATCATGGAGCTGAAGCCATTATGCCAACTATCTTTAATTTAATCCCGAAC
  AGTGCCAAAATTATGGCTACTTCTGGTGTTGTAGCTGTTAGGCTAATCATTCGGCACACTCACATCCCTC
  GGCTGATCCCTGTCATAACCAGCAACTGTACCTCTAAGTCTGTCGCCGTCAGAAGGCGCTGTTTTGAATT
  TTTAGATTTGCTTTTACAAGAATGGCAGACACATTCACTAGAAAGACATATATCAGTATTAGCTGAAACA
  ATAAAGAAAGGAATACACGACGCTGATTCTGAAGCGAGAATAGAAGCCAGGAAGTGCTACTGGGGTTTCC
  ACAGTCACTTCAGCCGAGAAGCAGAACACCTGTACCACACTTTGGAGTCCTCGTATCAGAAGGCCCTACA
  GTCCCACTTGAAGAACTCGGACAGCATCGTGTCTCTGCCCCAGTCAGACCGATCCTCTTCCAGCTCTCAA
  GAGAGTCTCAACCGGCCACTTTCAGCCAAAAGAAGTCCCACTGGCAGCACTGCATCCAGAGGCTCTACAG
  TTAGTACCAAATCTGTGTCGACGACAGGATCTCTCCAGCGATCTCGAAGTGATATTGATGTGAACGCAGC
  AGCCAGTGCCAAATCCAAAGTCTCCTCATCCTCAGGATCCCCCGCCTTCAGCTCTGCAGCAGCACTGCCC
  CCAGGATCCTATGCGTCCCTAGGTCGGATCCGCACGAGACGGCAGAGCTCGGGGAGCACCACCAATGTCG
  CCTCCACACCCTCCGACAGTCGGGGCCGCAGTCGCGCCAAAGTGGTTTCACAGTCTCAGCCTGGCAGCCG
  GTCAAGTTCCCCTGGGAAGCTTTTGGGAAGTGGACTTGCTGGGGGTTCCTCCAGAGGCCCACCGGTAACA
  CCATCCTCAGAAAAACGGAGCAAGATCCCCAGGAGTCAGGGATGTAGCCGAGAAACAAGTCCTAACCGGA
  TTGGATTAGATCGGTTTGGGCTGGGCCAGTCAGGACGAATCCCTGGTTCTGTGAACGCCATGAGAGTCTT
  GAGTACCAGCACTGACCTGGAAGCAGCAGTGGCTGACGCTCTGAAGAAGCCTGTGAGAAGGAGATACGAG
  CCCTATGGAATGTACTCTGATGATGATGCCAACAGTGATGCCTCCAGCGTGTGCTCTGAGCGCTCATATG
  GCTCCAGGAATGGTGGCATTCCCCATTATCTGCGGCAGACTGAAGATGTAGCAGAGGTTCTCAACCATTG
  TGCCAGTTCCAACTGGTCAGAACGGAAAGAGGGGCTCCTGGGCCTGCAGAACTTACTGAAGAGCCAAAGA
  ACACTGAGTCGAGTAGAATTGAAGAGACTGTGTGAGATTTTCACCCGAATGTTTGCCGACCCTCACAGCA
  AGAGAGTTTTCAGTATGTTTTTGGAGACCCTCGTGGATTTTATAATCATTCATAAGGATGACTTGCAAGA
  CTGGCTCTTCGTCCTTCTCACACAACTACTTAAGAAAATGGGAGCAGACTTACTTGGATCTGTGCAAGCA
  AAAGTTCAGAAGGCTCTCGATGTCACCAGGGATTCCTTTCCATTTGATCAACAATTTAACATTTTGATGA
  GATTTATTGTGGATCAGACTCAAACTCCAAACCTCAAGGTCAAAGTAGCAATCCTGAAATACATCGAATC
  TCTAGCCAGACAGATGGACCCCACAGATTTTGTAAATTCCAGCGAAACAAGACTAGCTGTTTCTAGGATC
  ATAACATGGACTACAGAACCAAAGAGTTCAGACGTGAGAAAGGCAGCACAAATTGTGCTCATCTCTCTGT
  TTGAACTGAACACGCCTGAATTTACCATGTTACTTGGTGCCTTGCCAAAAACATTCCAGGATGGTGCCAC
  CAAACTCCTGCATAACCACCTCAAGAACTCCAGTAACACCGGTGTGGGATCTCCAAGCAATACAATTGGC
  CGGACACCTTCCCGCCACCCCAGCAGCAGGACCAGCCCCCTGACCTCACCCACCAACTGTTCCCATGGGG
  GACTATCTCCAAGCATGCTGGACTATGATACAGAGAACCTGAACTCTGAAGAAATCTACAGCTCTTTGCG
  TGGAGTTACAGAAGCCATTGAAAAGTTCAGCTTCCGAAGCCAGGAGGATCTAAATGAGCCAATCAAACGA
  GATGGCAAGAAGGATTGTGATATCGTGTCCCGAGATGGGGGAGCAGCCTCACCTGCCACCGAGGGCCGGG
  GAGGTAGTGAGATAGAAGGAGGCAGGATGGCTTTGGACAACAAGACCTCCCTGCTCAACACGCAGCCTCC
  ACGTGCCTTTCCGGGGCCAAGAGCACGGGAATATAACCCGTATCCCTACTCCGACACCATCAACACCTAT
  GACAAGACGGCTCTGAAGGAAGCAGTGTTTGACGATGACATGGAGCAGCTCCGAGATGTGCCCATTGACC
  ACTCAGACCTGGTGGCTGACTTGCTGAAAGAGCTATCTAACCACAACGAGCGTGTGGAGGAGCGGAAGGG
  CGCACTGCTGGAGTTGCTCAAGATCACCAGGGAGGACAGCCTGGGCGTGTGGGAGGAGCACTTCAAGACC
  ATCCTGCTGCTGCTGCTGGAAACTCTCGGAGACAAAGACCATTCCATTCGAGCTCTGGCACTGAGAGTTT
  TACGGGAAATTCTGAGAAACCAGCCAGCAAGATTCAAAAACTATGCAGAACTGACGATCATGAAGACTCT
  GGAAGCCCACAAAGACTCCCACAAGGAGGTGGTGAGAGCGGCCGAGGAAGCTGCATCCACGCTAGCCAGC
  TCAATCCACCCAGAGCAGTGCATCAAAGTGCTGTGTCCAATCATCCAGACAGCCGACTACCCCATCAACC
  TGGCTGCTATCAAGATGCAGACAAAGGTGGTGGAGAGGATCACCAAGGAGTCCTTGCTGCAGCTCCTCGT
  CGACATCATCCCCGGCCTGCTGCAGGGTTACGACAACACCGAGAGCAGTGTACGGAAAGCCAGTGTGTTT
  TGCTTAGTGGCAATCTATTCCGTAATCGGAGAAGATCTGAAACCTCACCTCGCACAGCTCACGGGGAGCA
  AGATGAAGCTGCTGAACTTATATATAAAGAGGGCCCAGACTACCAACAGCAACAGCAGCTCTTCCTCTGA
  TGTGTCCACACACAGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001293301
• Insert Size: 4359 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293301.1, NP_001280230.1
• RefSeq Size: 7651 bp
• RefSeq ORF: 4359 bp
• Locus ID: 76707
• UniProt ID: Q80TV8
• Cytogenetics: 1 E2.3
• Gene Summary: Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) lacks five exons in the central coding regioin, but maintains the reading frame, compared to variant 4. The encoded isoform (5) is shorter, compared to isoform 4.