Kcnma1 (NM_001253374) Mouse Tagged ORF Clone

CAT#: MR231663

Kcnma1 (myc-DDK-tagged) - Mouse potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (Kcnma1), transcript variant 18

Product Images

Circular map for MR231663

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnma1
• Synonyms: 5730414M22Rik; BKCa; MaxiK; mSlo; mSlo1; Slo; Slo1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231663 representing NM_001253374
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAACGGTGGCGGCGGCGGCGGCGGCAGCAGCGGCGGCGGCGGCGGCGGCGGCGGAGGCAGCGGTC
  TTAGAATGAGCAGCAATATCCACGCGAACCATCTCAGCCTAGACGCGTCCTCCTCCTCCTCTTCCTCCTC
  CTCTTCTTCTTCTTCCTCCTCCTCTTCCTCCTCGTCCTCGGTCCACGAGCCCAAGATGGATGCGCTCATC
  ATACCGGTGACCATGGAGGTGCCGTGCGACAGCCGGGGCCAACGCATGTGGTGGGCTTTCTTGGCCTCCT
  CCATGGTGACTTTCTTCGGGGGCCTCTTCATCATCTTGCTCTGGCGGACGCTCAAGTACCTGTGGACCGT
  TTGCTGCCACTGCGGGGGCAAGACGAAGGTTGTGTTAGTCTTTGCTCTCAGCATTGGTGCCCTCGTAATA
  TACTTCATAGACTCGTCAAACCCAATAGAATCCTGCCAGAATTTCTACAAAGATTTCACATTACAGATCG
  ACATGGCTTTCAACGTGTTCTTCCTCCTCTACTTTGGCTTGCGGTTTATTGCAGCCAACGATAAGCTGTG
  GTTCTGGCTGGAAGTGAATTCAGTAGTAGATTTCTTCACAGTCCCTCCTGTGTTTGTGTCTGTGTACTTA
  AACAGAAGTTGGCTTGGCTTGAGATTTTTAAGAGCTCTCAGACTGATACAGTTTTCAGAGATTTTGCAGT
  TTCTGAATATCCTTAAAACAAGTAACTCCATCAAGCTGGTGAATCTGCTCTCCATATTTATCAGCACGTG
  GCTGACTGCAGCTGGATTCATCCACTTGGTGGAGAATTCAGGGGACCCATGGGAAAATTTCCAAAACAAC
  CAGGCACTTACGTACTGGGAATGTGTCTACTTACTCATGGTCACAATGTCTACAGTGGGTTATGGGGACG
  TTTATGCAAAAACCACACTTGGACGCCTCTTCATGGTCTTCTTCATCCTCGGGGGACTGGCCATGTTTGC
  CAGCTACGTCCCTGAAATCATAGAGTTAATAGGAAACCGCAAGAAATACGGGGGCTCCTATAGCGCGGTT
  AGTGGAAGAAAGCACATTGTAGTCTGTGGACACATTACTCTGGAGAGTGTCTCTAACTTCCTGAAGGACT
  TTCTGCACAAGGACCGGGATGATGTCAACGTGGAGATTGTCTTTCTTCACAACATCTCCCCTAACCTTGA
  GCTTGAGGCTCTGTTCAAACGGCATTTCACTCAGGTGGAGTTTTATCAGGGCTCTGTCCTCAATCCACAT
  GATCTTGCCAGAGTCAAGATAGAGTCAGCAGATGCATGCCTGATCCTTGCCAATAAGTATTGCGCTGACC
  CGGATGCAGAAGATGCCTCCAACATCATGAGAGTGATCTCCATCAAAAACTACCACCCAAAGATCAGGAT
  CATCACTCAGATGCTGCAGTATCACAACAAGGCCCATCTGCTCAACATCCCCAGCTGGAACTGGAAAGAG
  GGTGATGACGCAATATGCCTTGCAGAGCTCAAGTTGGGTTTCATAGCCCAGAGCTGTCTGGCTCAAGGCC
  TCTCCACAATGCTTGCCAATCTCTTCTCTATGAGGTCATTCATAAAGATTGAGGAAGACACATGGCAGAA
  ATACTACTTGGAAGGAGTCTCCAATGAAATGTACACAGAATATCTCTCCAGTGCCTTCGTGGGTCTGTCC
  TTCCCTACTGTTTGTGAGCTGTGTTTTGTGAAGCTTAAGCTCCTGATGATAGCCATTGAGTACAAGTCTG
  CCAACAGAGAGAGCCGAATATTAATTAACCCTGGGAACCACCTTAAGATCCAAGAAGGTACTTTAGGATT
  TTTCATCGCAAGTGATGCCAAAGAAGTTAAAAGGGCATTTTTTTACTGCAAGGCCTGTCATGATGACGTC
  ACAGATCCCAAAAGAATTAAAAAATGTGGCTGCAGGCGGCTGATCTATTTTGAAGATGAGCAGCCGCCAA
  CCCTGTCACCAAAAAAAAAACAACGTAATGGGGGCATGAGGAACTCGCCCAACACCTCCCCGAAGCTGAT
  GAGGCATGACCCCTTGTTAATTCCTGGCAATGATCAGATTGACAACATGGACTCCAATGTGAAAAAGTAC
  GACTCCACTGGAATGTTTCACTGGTGTGCACCCAAGGAGATTGAGAAAGTCATCTTGACTCGAAGTGAAG
  CTGCCATGACTGTCCTGAGTGGCCATGTCGTAGTCTGCATCTTTGGGGATGTCAGCTCAGCCCTGATTGG
  CCTCCGGAACCTGGTGATGCCACTTCGTGCTAGCAACTTTCACTATCATGAGCTCAAACACATTGTGTTT
  GTGGGCTCCATTGAGTACCTCAAGAGGGAGTGGGAAACACTGCACAACTTCCCGAAAGTGTCCATATTGC
  CTGGTACACCATTAAGTCGGGCTGATTTAAGGGCTGTCAACATCAACCTCTGTGACATGTGCGTTATCCT
  GTCAGCCAATCAGAATAATATTGATGATACTTCGCTTCAGGACAAGGAATGCATCTTGGCGTCACTCAAC
  ATCAAATCTATGCAGTTTGATGACAGCATCGGGGTCTTGCAGGCTAATTCCCAAGGATTCACACCTCCTG
  GAATGGACAGATCATCACCCGACAACAGCCCAGTGCACGGGATGTTACGCCAGCCGTCCATCACAACTGG
  GGTCAACATTCCCATCATCACGGAACTCGTGAATGATACCAATGTTCAGTTTTTGGACCAAGACGATGAC
  GATGACCCTGACACAGAGCTGTACCTCACACAGCCCTTTGCTTGTGGGACAGCATTTGCCGTCAGCGTCC
  TGGACTCACTCATGAGCGCGACATACTTCAATGACAATATCCTCACCCTAATACGGACCCTGGTGACAGG
  AGGAGCCACACCAGAGCTCGAGGCTCTAATAGCTGAGGAGAATGCACTTCGAGGAGGCTACAGCACTCCG
  CAGACATTGGCCAACAGGGACCGTTGCCGAGTGGCCCAGTTAGCCCTGTTAGATGGTCCCTTTGCAGACT
  TAGGGGATGGTGGTTGTTATGGTGATCTGTTCTGCAAAGCTCTGAAAACATATAATATGCTTTGTTTTGG
  AATTTACCGGCTGAGAGATGCCCACCTCAGCACCCCCAGCCAGTGTACAAAAAGGTACGTCATCACCAAT
  CCTCCCTACGAGTTTGAGCTCGTACCAACAGACCTGATCTTCTGCCTGATGCAGTTTGACCACAACGCTG
  GCCAATCCCGGGCCAGTCTGTCTCATTCCTCCCACTCCTCACAGTCGTCCAGTAAGAAGAGCTCCTCCGT
  CCACTCCATCCCGTCCACAGCAAATCGGCCGAACCGGCCCAAGTCCAGGGAGTCCCGCGACAAACAGAAT
  GCAACAAGGATGACTAGAATGGGCCAAGCAGAAAAGAAATGGTTTACAGATGAGCCGGATAATGCCTATC
  CCAGAAACATTCAAATCAAGCCCATGAGTACCCACATGGCTAACCAGATCAACCAATATAAATCCACAAG
  CAGCCTGATCCCTCCAATCAGAGAAGTTGAAGATGAATGT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231663 representing NM_001253374
  Red=Cloning site Green=Tags(s)
  
  MANGGGGGGGSSGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSVHEPKMDALI
  IPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKVVLVFALSIGALVI
  YFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYL
  NRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNN
  QALTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAV
  SGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPH
  DLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKE
  GDDAICLAELKLGFIAQSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLS
  FPTVCELCFVKLKLLMIAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDV
  TDPKRIKKCGCRRLIYFEDEQPPTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGNDQIDNMDSNVKKY
  DSTGMFHWCAPKEIEKVILTRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVF
  VGSIEYLKREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLN
  IKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDD
  DDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTP
  QTLANRDRCRVAQLALLDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITN
  PPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQN
  ATRMTRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001253374
• ORF Size: 3540 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253374.1, NP_001240303.1
• RefSeq Size: 4820 bp
• RefSeq ORF: 3543 bp
• Locus ID: 16531
• Cytogenetics: 14 A3
• MW: 132.2 kDa
• Gene Summary: Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).[UniProtKB/Swiss-Prot Function]