Kcnma1 (NM_001253374) Mouse Untagged Clone

CAT#: MC229452

Kcnma1 (untagged) - Mouse potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (Kcnma1), transcript variant 18

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcnma1
• Synonyms: 5730414M22Rik; BKCa; MaxiK; mSlo; mSlo1; Slo; Slo1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229452 representing NM_001253374
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAACGGTGGCGGCGGCGGCGGCGGCAGCAGCGGCGGCGGCGGCGGCGGCGGCGGAGGCAGCGGTC
  TTAGAATGAGCAGCAATATCCACGCGAACCATCTCAGCCTAGACGCGTCCTCCTCCTCCTCTTCCTCCTC
  CTCTTCTTCTTCTTCCTCCTCCTCTTCCTCCTCGTCCTCGGTCCACGAGCCCAAGATGGATGCGCTCATC
  ATACCGGTGACCATGGAGGTGCCGTGCGACAGCCGGGGCCAACGCATGTGGTGGGCTTTCTTGGCCTCCT
  CCATGGTGACTTTCTTCGGGGGCCTCTTCATCATCTTGCTCTGGCGGACGCTCAAGTACCTGTGGACCGT
  TTGCTGCCACTGCGGGGGCAAGACGAAGGTTGTGTTAGTCTTTGCTCTCAGCATTGGTGCCCTCGTAATA
  TACTTCATAGACTCGTCAAACCCAATAGAATCCTGCCAGAATTTCTACAAAGATTTCACATTACAGATCG
  ACATGGCTTTCAACGTGTTCTTCCTCCTCTACTTTGGCTTGCGGTTTATTGCAGCCAACGATAAGCTGTG
  GTTCTGGCTGGAAGTGAATTCAGTAGTAGATTTCTTCACAGTCCCTCCTGTGTTTGTGTCTGTGTACTTA
  AACAGAAGTTGGCTTGGCTTGAGATTTTTAAGAGCTCTCAGACTGATACAGTTTTCAGAGATTTTGCAGT
  TTCTGAATATCCTTAAAACAAGTAACTCCATCAAGCTGGTGAATCTGCTCTCCATATTTATCAGCACGTG
  GCTGACTGCAGCTGGATTCATCCACTTGGTGGAGAATTCAGGGGACCCATGGGAAAATTTCCAAAACAAC
  CAGGCACTTACGTACTGGGAATGTGTCTACTTACTCATGGTCACAATGTCTACAGTGGGTTATGGGGACG
  TTTATGCAAAAACCACACTTGGACGCCTCTTCATGGTCTTCTTCATCCTCGGGGGACTGGCCATGTTTGC
  CAGCTACGTCCCTGAAATCATAGAGTTAATAGGAAACCGCAAGAAATACGGGGGCTCCTATAGCGCGGTT
  AGTGGAAGAAAGCACATTGTAGTCTGTGGACACATTACTCTGGAGAGTGTCTCTAACTTCCTGAAGGACT
  TTCTGCACAAGGACCGGGATGATGTCAACGTGGAGATTGTCTTTCTTCACAACATCTCCCCTAACCTTGA
  GCTTGAGGCTCTGTTCAAACGGCATTTCACTCAGGTGGAGTTTTATCAGGGCTCTGTCCTCAATCCACAT
  GATCTTGCCAGAGTCAAGATAGAGTCAGCAGATGCATGCCTGATCCTTGCCAATAAGTATTGCGCTGACC
  CGGATGCAGAAGATGCCTCCAACATCATGAGAGTGATCTCCATCAAAAACTACCACCCAAAGATCAGGAT
  CATCACTCAGATGCTGCAGTATCACAACAAGGCCCATCTGCTCAACATCCCCAGCTGGAACTGGAAAGAG
  GGTGATGACGCAATATGCCTTGCAGAGCTCAAGTTGGGTTTCATAGCCCAGAGCTGTCTGGCTCAAGGCC
  TCTCCACAATGCTTGCCAATCTCTTCTCTATGAGGTCATTCATAAAGATTGAGGAAGACACATGGCAGAA
  ATACTACTTGGAAGGAGTCTCCAATGAAATGTACACAGAATATCTCTCCAGTGCCTTCGTGGGTCTGTCC
  TTCCCTACTGTTTGTGAGCTGTGTTTTGTGAAGCTTAAGCTCCTGATGATAGCCATTGAGTACAAGTCTG
  CCAACAGAGAGAGCCGAATATTAATTAACCCTGGGAACCACCTTAAGATCCAAGAAGGTACTTTAGGATT
  TTTCATCGCAAGTGATGCCAAAGAAGTTAAAAGGGCATTTTTTTACTGCAAGGCCTGTCATGATGACGTC
  ACAGATCCCAAAAGAATTAAAAAATGTGGCTGCAGGCGGCTGATCTATTTTGAAGATGAGCAGCCGCCAA
  CCCTGTCACCAAAAAAAAAACAACGTAATGGGGGCATGAGGAACTCGCCCAACACCTCCCCGAAGCTGAT
  GAGGCATGACCCCTTGTTAATTCCTGGCAATGATCAGATTGACAACATGGACTCCAATGTGAAAAAGTAC
  GACTCCACTGGAATGTTTCACTGGTGTGCACCCAAGGAGATTGAGAAAGTCATCTTGACTCGAAGTGAAG
  CTGCCATGACTGTCCTGAGTGGCCATGTCGTAGTCTGCATCTTTGGGGATGTCAGCTCAGCCCTGATTGG
  CCTCCGGAACCTGGTGATGCCACTTCGTGCTAGCAACTTTCACTATCATGAGCTCAAACACATTGTGTTT
  GTGGGCTCCATTGAGTACCTCAAGAGGGAGTGGGAAACACTGCACAACTTCCCGAAAGTGTCCATATTGC
  CTGGTACACCATTAAGTCGGGCTGATTTAAGGGCTGTCAACATCAACCTCTGTGACATGTGCGTTATCCT
  GTCAGCCAATCAGAATAATATTGATGATACTTCGCTTCAGGACAAGGAATGCATCTTGGCGTCACTCAAC
  ATCAAATCTATGCAGTTTGATGACAGCATCGGGGTCTTGCAGGCTAATTCCCAAGGATTCACACCTCCTG
  GAATGGACAGATCATCACCCGACAACAGCCCAGTGCACGGGATGTTACGCCAGCCGTCCATCACAACTGG
  GGTCAACATTCCCATCATCACGGAACTCGTGAATGATACCAATGTTCAGTTTTTGGACCAAGACGATGAC
  GATGACCCTGACACAGAGCTGTACCTCACACAGCCCTTTGCTTGTGGGACAGCATTTGCCGTCAGCGTCC
  TGGACTCACTCATGAGCGCGACATACTTCAATGACAATATCCTCACCCTAATACGGACCCTGGTGACAGG
  AGGAGCCACACCAGAGCTCGAGGCTCTAATAGCTGAGGAGAATGCACTTCGAGGAGGCTACAGCACTCCG
  CAGACATTGGCCAACAGGGACCGTTGCCGAGTGGCCCAGTTAGCCCTGTTAGATGGTCCCTTTGCAGACT
  TAGGGGATGGTGGTTGTTATGGTGATCTGTTCTGCAAAGCTCTGAAAACATATAATATGCTTTGTTTTGG
  AATTTACCGGCTGAGAGATGCCCACCTCAGCACCCCCAGCCAGTGTACAAAAAGGTACGTCATCACCAAT
  CCTCCCTACGAGTTTGAGCTCGTACCAACAGACCTGATCTTCTGCCTGATGCAGTTTGACCACAACGCTG
  GCCAATCCCGGGCCAGTCTGTCTCATTCCTCCCACTCCTCACAGTCGTCCAGTAAGAAGAGCTCCTCCGT
  CCACTCCATCCCGTCCACAGCAAATCGGCCGAACCGGCCCAAGTCCAGGGAGTCCCGCGACAAACAGAAT
  GCAACAAGGATGACTAGAATGGGCCAAGCAGAAAAGAAATGGTTTACAGATGAGCCGGATAATGCCTATC
  CCAGAAACATTCAAATCAAGCCCATGAGTACCCACATGGCTAACCAGATCAACCAATATAAATCCACAAG
  CAGCCTGATCCCTCCAATCAGAGAAGTTGAAGATGAATGTTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001253374
• Insert Size: 3543 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253374.1, NP_001240303.1
• RefSeq Size: 4820 bp
• RefSeq ORF: 3543 bp
• Locus ID: 16531
• Cytogenetics: 14 A3
• Gene Summary: Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (18) lacks two in-frame exons in the coding region, compared to variant 1. The resulting isoform (18) lacks two internal segments, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.