Gak (NM_001282051) Mouse Tagged ORF Clone

CAT#: MR231619

Gak (myc-DDK-tagged) - Mouse cyclin G associated kinase (Gak), transcript variant 2

Product Images

Circular map for MR231619

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gak
• Synonyms: D130045N16Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231619 representing NM_001282051
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACAGGCAGAAACCACCCATCATCCACAGAGATCTCAAGGTTGAAAACTTACTGCTTAGTAACCAGG
  GGACCATTAAGCTGTGTGACTTTGGCAGTGCCACAACCATCTCCCATTATCCTGACTACAGCTGGAGTGC
  CCAGAAGCGAGCCATGGTGGAGGAAGAGATCACAAGGAACACCACACCCATGTACAGAACACCAGAAATT
  GTAGACCTGTATTCCAACTTCCCCATTGGCGAAAAGCAGGATATCTGGGCACTGGGCTGTATCTTATACC
  TGCTGTGTTTCCGGCAGCATCCTTTTGAGGATGGAGCAAAACTTCGGATAGTCAATGGGAAGTATTCCAT
  TCCTGTGAATGACACTCGTTACACAGTCTTCCATGACCTTATTCGTGCCATGCTAAAGGTCAACCCAGAG
  GAGAGGCTGTCCATTGCTGAAGTGGTCCGACAACTGCAGGAAATTGCAGCAGCTAGGAATGTGAACCCCA
  AAGCTCCCATCACAGAGCTGCTGGAGCAGAATGGTGGCTATGGGAACTCAGGGCCTTCCCGAGCACAACC
  ACCTTGCGGGGGCACCGTGAACAGCAGTGGAGTTTTGGCTCTGGCAGAGTATGACCAGCCCTACGGTGGG
  TTTCTTGATATTCTGCGGGGTGGGACCGAACGGCTCTTCACCAACCTCAAGGATACTTCCTCCAAGGTCA
  TCCAGTCTGTGGCTAACTATGCAAAGGGTGATCTGGACATATCTTACATCACATCCAGGATTGCAGTGAT
  GTCATTCCCAGCAGAAGGTGTGGAGTCTGCAATCAAAAACAATATAGAGGATGTGCGAATGTTTCTGGAT
  GCTAAGCATCCAGGACATTATGCTGTCTACAACCTTTCTCCAAGGATATACCGGGCTTCCAAGTTTCACA
  ACCGGGTCACTGAGTGTGGCTGGGCAGTCAGACGGGCACCACATCTCCACAGTTTGTATACTCTATGTAG
  GAGCATGCATGCCTGGCTCCGGGAAGACCACAGGAACGTCTGTGTTGTGCATTGCATGGATGGGAGAGCT
  GCATCTGCTGTGGCAGTCTGTGCATTCCTGTGCTTCTGCCGTCTCTTCAGCACTGCAGAGGCTGCTGTGT
  ACATGTTCAGCATGAAGCGCTGCCCACCAGGCATTTGGCCATCCCACAAAAGGTACATTGAATATGTATG
  TGACATGGTGGCAGAGGAGCCCATCACGCCCCATAGCAAGCCAATGCTGGTGAAATCTGTTGTCATGACC
  CCCGTGCCACTGTTCAGCAAGCAGAGGAATGGCTGCCGACCGTTCTGTGAGGTCTACGTTGGAGAGGAGC
  GTGTCACCACCACGTCGCAGGAATATGACAGAATGAAGGAATTTAAAATTGAGGATGGCAAGGCTGTCAT
  CCCCCTGGGCGTAACAGTTCAAGGAGACGTGCTCATCATCATCTACCATGCCAGGGCCACACTGGGAGGG
  AGGCTGCAGGCTAAGATGGCGTCCATGAAAATGTTCCAGATCCAGTTCCACACTGGGTTCGTGCCTCGAA
  ACGCAACCACTGTGAAATTTGCAAAATATGACCTGGATGCTTGTGATATTCAAGAGAAGTACCCAGATCT
  GTTCCAGGTGAACCTGGAAGTGGAGGTAGAGCCTAGAGACAGGCCCAGCCGAGAAGCTCCACCTTGGGAG
  AACACCAGCCTAAGGGGGTTAAACCCCAAGATCCTCTTTTCCAACAGGGAAGAGCAGCAGGACATTCTGT
  CTAAGTTTGGGAAGCCGGAGCTACCCCGGCAGCCGGGCTCCACAGCTCAGTATGATGCTGAGGCAGGGTC
  TCCGGAGGCTGAGATCACAGAGTCGGACTCACCGCAGAGCAGCAGTACGGACACCAACCACTTTCTTCAC
  ACACTGGATTGGCAGGAGGAAAAAGAACCAGAGACTGGGTTAGACAATACCTCTCCTAAGGAGAGTCAGT
  CTGTCCTGATTGCAGACGGAGATGGAAGTGAAGTATCAGATGAAGAAGAGGCTTCATTCCCCAGTGAGGA
  GAGGAAACCAGGAGCTGGAGAAGATACACCAAGGCTGGCTGCTGGGACCAAACAGCAAGACTTAATATTT
  GATGTGGGCATGCTGGCTGCCCCACAGGAGCCTGTACAGCCCGAAGAAGGTGTTGATCTCCTGGGGCTAC
  ACTCTGAGGGGGACTTAAGGCCTGCTGCCCCCTTACAGGCTTGTGGGGTCCCATCTAGCAACACTGACCT
  GTTGAGCTGCCTTCTTGAACCATCTGATGCTGCTCAAGTAGGGCCTCCTGGTGACCTGCTTGGTGGTGAG
  GCTCCACTGCTGCTAGCAAGCCCAGTTTCTCCTCTTGGGCTGCAGAACAACCTACAAGGAAAAGTCCCTG
  ACACTGTGGACCCATTTGACCAGTTCCTGCTGTCATCCAACTCAGACACCCAGCCCTGCTCCAAGCCTGA
  TCTCTTTGGAGAGTTTCTCAACTCTGACTCTGTAGCTTCCTCAACTGCCTTCCCATCCACCCACAGTGCC
  CCACCCCCATCCTGTAGCACTGCCTTCCTGCACCTAGGGGATCTGCCAGCAGAGCCCAGCAAGGTAATAG
  CTTCATCCAGCCACCCAGATCTGCTAGGAGGATGGGATACGTGGGCTGACACAGCTACACCTGGGCCAGC
  CTCCATTCCAGTACCAGAAGGTACCCTCTTCTCTTCTGCAGGTCACCCAGCCCCTCCTGGCCCCAACCCC
  AGCCAAACCAAGTCTCAGAACCTTGACCCATTTGCTGATCTCAGTGATCTCAGCTCTAGCCTCCAAGGCC
  TGCCTGCTGGACTTCCTGCAGGGGGCTTTGTTGGCGCACCAGCCCCCACTCAAAAGAGCAACAGCCCCTG
  GCAGGCAAATCGTCCCACAGCCCCTGGAACCTCATGGACCCCCCAGGCCAAGCCAGCCCCCAGAGCCTCT
  GAACAGCTAAGGTCTCACTTCAGTGTGATCGGGGCCCGAGAAGAGAGGGGCGTCCGTGTGCCCAGCTTTG
  CCCAAAAGCCAAAGGTCTCAGAAAATGATTTTGAAGATCTTCTGCCTAATCAAGGCTTCTCTAAGTCTGA
  CAAGAAGGGGCCAAAGACCATGGCAGAGATGCGGAAACAGGAACTTGCCAGAGATACAGACCCACTCAAA
  TTGAAGCTTTTGGACTGGATTGAAGGCAAGGAGAGGAACATTCGTGCACTGCTGTCCACTCTGCACACGG
  TATTGTGGGATGGGGAGAGCCGCTGGACACCTGTGAGTATGGCTGACCTGGTGACTCCAGAGCAGGTGAA
  GAAGCAGTACCGCCGTGCAGTGCTGGTAGTGCATCCTGATAAGGCCACAGGGCAGCCGTATGAACAGTAT
  GCCAAGATGATCTTCATGGAGCTGAATGATGCATGGTCTGAGTTTGAAAACCAGGGCTCGAGGCCCCTCT
  TC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231619 representing NM_001282051
  Red=Cloning site Green=Tags(s)
  
  MHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQKRAMVEEEITRNTTPMYRTPEI
  VDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPVNDTRYTVFHDLIRAMLKVNPE
  ERLSIAEVVRQLQEIAAARNVNPKAPITELLEQNGGYGNSGPSRAQPPCGGTVNSSGVLALAEYDQPYGG
  FLDILRGGTERLFTNLKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRMFLD
  AKHPGHYAVYNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSMHAWLREDHRNVCVVHCMDGRA
  ASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDMVAEEPITPHSKPMLVKSVVMT
  PVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDGKAVIPLGVTVQGDVLIIIYHARATLGG
  RLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSREAPPWE
  NTSLRGLNPKILFSNREEQQDILSKFGKPELPRQPGSTAQYDAEAGSPEAEITESDSPQSSSTDTNHFLH
  TLDWQEEKEPETGLDNTSPKESQSVLIADGDGSEVSDEEEASFPSEERKPGAGEDTPRLAAGTKQQDLIF
  DVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQACGVPSSNTDLLSCLLEPSDAAQVGPPGDLLGGE
  APLLLASPVSPLGLQNNLQGKVPDTVDPFDQFLLSSNSDTQPCSKPDLFGEFLNSDSVASSTAFPSTHSA
  PPPSCSTAFLHLGDLPAEPSKVIASSSHPDLLGGWDTWADTATPGPASIPVPEGTLFSSAGHPAPPGPNP
  SQTKSQNLDPFADLSDLSSSLQGLPAGLPAGGFVGAPAPTQKSNSPWQANRPTAPGTSWTPQAKPAPRAS
  EQLRSHFSVIGAREERGVRVPSFAQKPKVSENDFEDLLPNQGFSKSDKKGPKTMAEMRKQELARDTDPLK
  LKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQYRRAVLVVHPDKATGQPYEQY
  AKMIFMELNDAWSEFENQGSRPLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282051
• ORF Size: 3432 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282051.1, NP_001268980.1
• RefSeq Size: 4793 bp
• RefSeq ORF: 3435 bp
• Locus ID: 231580
• UniProt ID: Q99KY4
• Cytogenetics: 5 F
• MW: 126.5 kDa
• Gene Summary: Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 (By similarity).[UniProtKB/Swiss-Prot Function]