Gak (NM_001282051) Mouse Untagged Clone

CAT#: MC229408

Gak (untagged) - Mouse cyclin G associated kinase (Gak), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gak
• Synonyms: D130045N16Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229408 representing NM_001282051
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACAGGCAGAAACCACCCATCATCCACAGAGATCTCAAGGTTGAAAACTTACTGCTTAGTAACCAGG
  GGACCATTAAGCTGTGTGACTTTGGCAGTGCCACAACCATCTCCCATTATCCTGACTACAGCTGGAGTGC
  CCAGAAGCGAGCCATGGTGGAGGAAGAGATCACAAGGAACACCACACCCATGTACAGAACACCAGAAATT
  GTAGACCTGTATTCCAACTTCCCCATTGGCGAAAAGCAGGATATCTGGGCACTGGGCTGTATCTTATACC
  TGCTGTGTTTCCGGCAGCATCCTTTTGAGGATGGAGCAAAACTTCGGATAGTCAATGGGAAGTATTCCAT
  TCCTGTGAATGACACTCGTTACACAGTCTTCCATGACCTTATTCGTGCCATGCTAAAGGTCAACCCAGAG
  GAGAGGCTGTCCATTGCTGAAGTGGTCCGACAACTGCAGGAAATTGCAGCAGCTAGGAATGTGAACCCCA
  AAGCTCCCATCACAGAGCTGCTGGAGCAGAATGGTGGCTATGGGAACTCAGGGCCTTCCCGAGCACAACC
  ACCTTGCGGGGGCACCGTGAACAGCAGTGGAGTTTTGGCTCTGGCAGAGTATGACCAGCCCTACGGTGGG
  TTTCTTGATATTCTGCGGGGTGGGACCGAACGGCTCTTCACCAACCTCAAGGATACTTCCTCCAAGGTCA
  TCCAGTCTGTGGCTAACTATGCAAAGGGTGATCTGGACATATCTTACATCACATCCAGGATTGCAGTGAT
  GTCATTCCCAGCAGAAGGTGTGGAGTCTGCAATCAAAAACAATATAGAGGATGTGCGAATGTTTCTGGAT
  GCTAAGCATCCAGGACATTATGCTGTCTACAACCTTTCTCCAAGGATATACCGGGCTTCCAAGTTTCACA
  ACCGGGTCACTGAGTGTGGCTGGGCAGTCAGACGGGCACCACATCTCCACAGTTTGTATACTCTATGTAG
  GAGCATGCATGCCTGGCTCCGGGAAGACCACAGGAACGTCTGTGTTGTGCATTGCATGGATGGGAGAGCT
  GCATCTGCTGTGGCAGTCTGTGCATTCCTGTGCTTCTGCCGTCTCTTCAGCACTGCAGAGGCTGCTGTGT
  ACATGTTCAGCATGAAGCGCTGCCCACCAGGCATTTGGCCATCCCACAAAAGGTACATTGAATATGTATG
  TGACATGGTGGCAGAGGAGCCCATCACGCCCCATAGCAAGCCAATGCTGGTGAAATCTGTTGTCATGACC
  CCCGTGCCACTGTTCAGCAAGCAGAGGAATGGCTGCCGACCGTTCTGTGAGGTCTACGTTGGAGAGGAGC
  GTGTCACCACCACGTCGCAGGAATATGACAGAATGAAGGAATTTAAAATTGAGGATGGCAAGGCTGTCAT
  CCCCCTGGGCGTAACAGTTCAAGGAGACGTGCTCATCATCATCTACCATGCCAGGGCCACACTGGGAGGG
  AGGCTGCAGGCTAAGATGGCGTCCATGAAAATGTTCCAGATCCAGTTCCACACTGGGTTCGTGCCTCGAA
  ACGCAACCACTGTGAAATTTGCAAAATATGACCTGGATGCTTGTGATATTCAAGAGAAGTACCCAGATCT
  GTTCCAGGTGAACCTGGAAGTGGAGGTAGAGCCTAGAGACAGGCCCAGCCGAGAAGCTCCACCTTGGGAG
  AACACCAGCCTAAGGGGGTTAAACCCCAAGATCCTCTTTTCCAACAGGGAAGAGCAGCAGGACATTCTGT
  CTAAGTTTGGGAAGCCGGAGCTACCCCGGCAGCCGGGCTCCACAGCTCAGTATGATGCTGAGGCAGGGTC
  TCCGGAGGCTGAGATCACAGAGTCGGACTCACCGCAGAGCAGCAGTACGGACACCAACCACTTTCTTCAC
  ACACTGGATTGGCAGGAGGAAAAAGAACCAGAGACTGGGTTAGACAATACCTCTCCTAAGGAGAGTCAGT
  CTGTCCTGATTGCAGACGGAGATGGAAGTGAAGTATCAGATGAAGAAGAGGCTTCATTCCCCAGTGAGGA
  GAGGAAACCAGGAGCTGGAGAAGATACACCAAGGCTGGCTGCTGGGACCAAACAGCAAGACTTAATATTT
  GATGTGGGCATGCTGGCTGCCCCACAGGAGCCTGTACAGCCCGAAGAAGGTGTTGATCTCCTGGGGCTAC
  ACTCTGAGGGGGACTTAAGGCCTGCTGCCCCCTTACAGGCTTGTGGGGTCCCATCTAGCAACACTGACCT
  GTTGAGCTGCCTTCTTGAACCATCTGATGCTGCTCAAGTAGGGCCTCCTGGTGACCTGCTTGGTGGTGAG
  GCTCCACTGCTGCTAGCAAGCCCAGTTTCTCCTCTTGGGCTGCAGAACAACCTACAAGGAAAAGTCCCTG
  ACACTGTGGACCCATTTGACCAGTTCCTGCTGTCATCCAACTCAGACACCCAGCCCTGCTCCAAGCCTGA
  TCTCTTTGGAGAGTTTCTCAACTCTGACTCTGTAGCTTCCTCAACTGCCTTCCCATCCACCCACAGTGCC
  CCACCCCCATCCTGTAGCACTGCCTTCCTGCACCTAGGGGATCTGCCAGCAGAGCCCAGCAAGGTAATAG
  CTTCATCCAGCCACCCAGATCTGCTAGGAGGATGGGATACGTGGGCTGACACAGCTACACCTGGGCCAGC
  CTCCATTCCAGTACCAGAAGGTACCCTCTTCTCTTCTGCAGGTCACCCAGCCCCTCCTGGCCCCAACCCC
  AGCCAAACCAAGTCTCAGAACCTTGACCCATTTGCTGATCTCAGTGATCTCAGCTCTAGCCTCCAAGGCC
  TGCCTGCTGGACTTCCTGCAGGGGGCTTTGTTGGCGCACCAGCCCCCACTCAAAAGAGCAACAGCCCCTG
  GCAGGCAAATCGTCCCACAGCCCCTGGAACCTCATGGACCCCCCAGGCCAAGCCAGCCCCCAGAGCCTCT
  GAACAGCTAAGGTCTCACTTCAGTGTGATCGGGGCCCGAGAAGAGAGGGGCGTCCGTGTGCCCAGCTTTG
  CCCAAAAGCCAAAGGTCTCAGAAAATGATTTTGAAGATCTTCTGCCTAATCAAGGCTTCTCTAAGTCTGA
  CAAGAAGGGGCCAAAGACCATGGCAGAGATGCGGAAACAGGAACTTGCCAGAGATACAGACCCACTCAAA
  TTGAAGCTTTTGGACTGGATTGAAGGCAAGGAGAGGAACATTCGTGCACTGCTGTCCACTCTGCACACGG
  TATTGTGGGATGGGGAGAGCCGCTGGACACCTGTGAGTATGGCTGACCTGGTGACTCCAGAGCAGGTGAA
  GAAGCAGTACCGCCGTGCAGTGCTGGTAGTGCATCCTGATAAGGCCACAGGGCAGCCGTATGAACAGTAT
  GCCAAGATGATCTTCATGGAGCTGAATGATGCATGGTCTGAGTTTGAAAACCAGGGCTCGAGGCCCCTCT
  TCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001282051
• Insert Size: 3435 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282051.1, NP_001268980.1
• RefSeq Size: 4793 bp
• RefSeq ORF: 3435 bp
• Locus ID: 231580
• UniProt ID: Q99KY4
• Cytogenetics: 5 F
• Gene Summary: Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in its 5' UTR and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2 and 6 both encode the same isoform (2).