Asap1 (NM_001276463) Mouse Tagged ORF Clone

CAT#: MR231588

Asap1 (myc-DDK-tagged) - Mouse ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 4

Product Images

Circular map for MR231588

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Asap1
• Synonyms: AV239055; Ddef1; DEF-1; mKIAA1249; PAP; s19
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231588 representing NM_001276463
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGACCAGATCTCCGTGTCCGAGTTCATCGCCGAGACCACCGAGGACTACAACTCGCCCACCACGT
  CCAGCTTCACCACGCGGCTGCACAACTGCAGGAACACCGTCACGCTGCTGGAGGAGGCTCTAGACCAAGA
  TAGAACAGCCCTACAGAAAGTGAAGAAATCTGTAAAAGCAATATATAATTCCGGTCAAGATCATGTACAA
  AATGAAGAAAACTATGCACAAGTTCTGGATAAGTTTGGAAGTAATTTTTTAAGCAGAGACAACCCTGACC
  TTGGCACCGCTTTTGTCAAGTTTTCTACACTCACAAAGGAACTGTCCACGCTGCTGAAAAATCTGCTCCA
  GGGTCTGAGCCACAATGTGATCTTCACCTTGGATTCCTTGTTAAAAGGAGACTTGAAGGGGGTCAAAGGA
  GATCTCAAGAAGCCATTTGACAAAGCCTGGAAAGATTATGAGACGAAGTTTACAAAAATTGAGAAGGAGA
  AAAGAGAGCACGCAAAACAGCACGGGATGATCCGAACAGAGATAACAGGCGCTGAGATTGCAGAAGAAAT
  GGAAAAGGAACGGCGGCTTTTTCAGCTCCAGATGTGTGAATATCTCATTAAAGTAAATGAAATCAAGACC
  AAAAAGGGTGTGGACCTGCTGCAGAACCTTATAAAGTATTACCATGCACAGTGCAATTTCTTTCAGGATG
  GCTTGAAAACGGCTGATAAACTGAAACAGTACATAGAAAAGCTGGCTGCTGACTTATATAATATAAAGCA
  AACCCAAGATGAAGAAAAAAAACAGCTAACTGCACTCCGAGATCTAATAAAATCCTCTCTGCAACTTGAT
  CCGAAAGAAGACTCTCAGAGCCGGCAAGGTGGGTACAGCATGCATCAGCTCCAGGGCAACAAGGAGTACG
  GCAGTGAAAAGAAGGGCTTCCTGCTGAAGAAGAGTGATGGGATCCGGAAAGTGTGGCAGAGACGGAAGTG
  TGCGGTCAAGAACGGGATCCTGACCATCTCCCACGCAACTTCCAACAGGCAGCCTGCTAAGTTAAACCTC
  CTCACCTGCCAAGTGAAACCGAATGCTGAGGACAAGAAGTCTTTTGACCTGATATCACATAATCGAACAT
  ATCACTTTCAAGCAGAAGATGAGCAGGATTATATAGCGTGGATATCAGTACTGACGAATAGCAAAGAAGA
  GGCCCTAACCATGGCCTTCCGTGGTGAGCAGAGCACAGGGGAGAATAGCCTGGAGGACCTTACCAAAGCC
  ATCATCGAGGATGTACAGCGGCTCCCTGGGAATGACATCTGCTGTGACTGCGGCTCATCAGAACCCACGT
  GGCTTTCAACCAACTTGGGTATTTTGACCTGTATAGAATGTTCCGGAATCCATAGGGAAATGGGGGTTCA
  TATTTCTCGCATTCAGTCTTTGGAACTAGACAAATTAGGAACTTCCGAACTCTTGCTGGCCAAGAATGTA
  GGAAACAATAGTTTTAATGATATTATGGAAGCAAATTTACCCAGCCCTTCACCAAAGCCCACCCCTTCAA
  GTGATATGACCGTGCGTAAAGAGTACATCACTGCAAAGTATGTCGACCACCGCTTCTCCAGGAAGACCTG
  TGCCTCTTCCTCAGCAAAGCTGAACGAATTGCTTGAGGCCATCAAGTCCAGGGACTTACTTGCATTAATC
  CAAGTCTATGCAGAGGGGGTGGAGTTAATGGAGCCACTGCTGGAACCTGGGCAGGAGCTCGGGGAGACAG
  CCCTTCATCTTGCCGTCAGGACCGCAGACCAGACATCTCTCCATCTGGTTGATTTCCTTGTACAAAACTG
  TGGGAACCTGGATAAGCAAACGTCTGTGGGGAATACAGTTCTGCACTATTGCAGCATGTACGGCAAGCCG
  GAGTGTCTGAAGCTGCTGCTTAGGAGCAAGCCCACCGTGGACATCGTTAACCAGAATGGAGAAACTGCCT
  TGGATATAGCAAAGAGACTCAAAGCTACCCAGTGTGAAGATCTGCTTTCCCAGGCTAAATCTGGAAAGTT
  CAACCCTCATGTCCACGTAGAGTATGAGTGGAATCTTCGACAGGACGAGATGGATGAGAGCGATGACGAT
  CTGGATGACAAGCCAAGCCCGATCAAAAAGGAACGCTCGCCCAGACCACAGAGCTTCTGCCACTCCTCCA
  GCATCTCGCCACAGGACAAGCTGGCACTGCCAGGGTTCAGCACTCCACGGGACAAGCAGCGGCTCTCCTA
  CGGAGCCTTCACCAACCAGATCTTCGCCTCTACGAGCACAGATTTGCCCACATCACCCACCAGTGAGGCT
  CCCCCTTTGCCACCTCGGAACGCCGGGAAAGGTCCAACTGGCCCACCTTCAACACTCCCTCTAGGCACCC
  AGACCTCTAGTGGCAGCTCCACCCTATCCAAGAAGAGGCCTCCTCCCCCACCACCAGGACACAAGAGAAC
  CCTGTCTGACCCTCCCAGCCCACTACCTCACGGGCCCCCAAACAAAGGCGCAATTCCTTGGGGTAATGAT
  GTGGGCCCATTATCTTCAAGTAAGACGGCCAACAAGTTTGAGGGGCTGTCTCAGCAAGCAAGCACCAGTT
  CTGCTAAGACTGCCCTTGGCCCGAGAGTGCTTCCTAAACTACCTCAGAAAGTGGCACTAAGGAAGACGGA
  GACCAGCCATCATCTCTCCCTCGACAGAACCAACATCCCACCTGAGACTTTTCAGAAATCATCACAGTTG
  ACAGAGTTACCCCAAAAGCCACCACTTGGAGAGCTGCCCCCGAAGCCTGTGGAACTGGCCCCCAAGCCCC
  AAGTTGGAGAGCTGCCACCTAAGCCTGGAGAGCTACCCCCTAAGCCCCAATTAGGTGACCTGCCCCCCAA
  GCCACAGCTCTCAGACTTACCTCCCAAGCCACAGATGAAGGACCTGCCCCCTAAGCCGCAGCTGGGGGAT
  CTGCTGGCAAAGTCCCAGGCTGGCGATGTCTCAGCCAAGGTGCAGCCACCCTCAGAGGTCACACAGAGGT
  CACACACCGGGGATCTGTCTCCAAATGTACAGTCCAGAGATGCCATCCAGAAGCAAGCATCTGAAGACTC
  CAACGACCTCACACCCACGCTGCCAGAGACACCCGTACCACTGCCGAGAAAAATCAATACGGGGAAAAAT
  AAAGTGAGGCGGGTGAAGACCATTTATGACTGCCAGGCAGATAATGACGACGAACTCACATTTATTGAGG
  GGGAAGTGATCATTGTCACCGGGGAAGAGGACCAGGAGTGGTGGATCGGGCATATCGAAGGACAGCCTGA
  AAGGAAGGGTGTCTTTCCAGTGTCCTTTGTCCACATCCTGTCTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231588 representing NM_001276463
  Red=Cloning site Green=Tags(s)
  
  MPDQISVSEFIAETTEDYNSPTTSSFTTRLHNCRNTVTLLEEALDQDRTALQKVKKSVKAIYNSGQDHVQ
  NEENYAQVLDKFGSNFLSRDNPDLGTAFVKFSTLTKELSTLLKNLLQGLSHNVIFTLDSLLKGDLKGVKG
  DLKKPFDKAWKDYETKFTKIEKEKREHAKQHGMIRTEITGAEIAEEMEKERRLFQLQMCEYLIKVNEIKT
  KKGVDLLQNLIKYYHAQCNFFQDGLKTADKLKQYIEKLAADLYNIKQTQDEEKKQLTALRDLIKSSLQLD
  PKEDSQSRQGGYSMHQLQGNKEYGSEKKGFLLKKSDGIRKVWQRRKCAVKNGILTISHATSNRQPAKLNL
  LTCQVKPNAEDKKSFDLISHNRTYHFQAEDEQDYIAWISVLTNSKEEALTMAFRGEQSTGENSLEDLTKA
  IIEDVQRLPGNDICCDCGSSEPTWLSTNLGILTCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNV
  GNNSFNDIMEANLPSPSPKPTPSSDMTVRKEYITAKYVDHRFSRKTCASSSAKLNELLEAIKSRDLLALI
  QVYAEGVELMEPLLEPGQELGETALHLAVRTADQTSLHLVDFLVQNCGNLDKQTSVGNTVLHYCSMYGKP
  ECLKLLLRSKPTVDIVNQNGETALDIAKRLKATQCEDLLSQAKSGKFNPHVHVEYEWNLRQDEMDESDDD
  LDDKPSPIKKERSPRPQSFCHSSSISPQDKLALPGFSTPRDKQRLSYGAFTNQIFASTSTDLPTSPTSEA
  PPLPPRNAGKGPTGPPSTLPLGTQTSSGSSTLSKKRPPPPPPGHKRTLSDPPSPLPHGPPNKGAIPWGND
  VGPLSSSKTANKFEGLSQQASTSSAKTALGPRVLPKLPQKVALRKTETSHHLSLDRTNIPPETFQKSSQL
  TELPQKPPLGELPPKPVELAPKPQVGELPPKPGELPPKPQLGDLPPKPQLSDLPPKPQMKDLPPKPQLGD
  LLAKSQAGDVSAKVQPPSEVTQRSHTGDLSPNVQSRDAIQKQASEDSNDLTPTLPETPVPLPRKINTGKN
  KVRRVKTIYDCQADNDDELTFIEGEVIIVTGEEDQEWWIGHIEGQPERKGVFPVSFVHILSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276463
• ORF Size: 3336 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276463.1, NP_001263392.1
• RefSeq Size: 6145 bp
• RefSeq ORF: 3339 bp
• Locus ID: 13196
• Cytogenetics: 15 D1
• MW: 124 kDa
• Gene Summary: May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types. Plays a role in ciliogenesis (By similarity). Posseses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2.[UniProtKB/Swiss-Prot Function]