Asap1 (NM_001276463) Mouse Untagged Clone

CAT#: MC229377

Asap1 (untagged) - Mouse ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Asap1
• Synonyms: AV239055; Ddef1; DEF-1; mKIAA1249; PAP; s19
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229377 representing NM_001276463
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGACCAGATCTCCGTGTCCGAGTTCATCGCCGAGACCACCGAGGACTACAACTCGCCCACCACGT
  CCAGCTTCACCACGCGGCTGCACAACTGCAGGAACACCGTCACGCTGCTGGAGGAGGCTCTAGACCAAGA
  TAGAACAGCCCTACAGAAAGTGAAGAAATCTGTAAAAGCAATATATAATTCCGGTCAAGATCATGTACAA
  AATGAAGAAAACTATGCACAAGTTCTGGATAAGTTTGGAAGTAATTTTTTAAGCAGAGACAACCCTGACC
  TTGGCACCGCTTTTGTCAAGTTTTCTACACTCACAAAGGAACTGTCCACGCTGCTGAAAAATCTGCTCCA
  GGGTCTGAGCCACAATGTGATCTTCACCTTGGATTCCTTGTTAAAAGGAGACTTGAAGGGGGTCAAAGGA
  GATCTCAAGAAGCCATTTGACAAAGCCTGGAAAGATTATGAGACGAAGTTTACAAAAATTGAGAAGGAGA
  AAAGAGAGCACGCAAAACAGCACGGGATGATCCGAACAGAGATAACAGGCGCTGAGATTGCAGAAGAAAT
  GGAAAAGGAACGGCGGCTTTTTCAGCTCCAGATGTGTGAATATCTCATTAAAGTAAATGAAATCAAGACC
  AAAAAGGGTGTGGACCTGCTGCAGAACCTTATAAAGTATTACCATGCACAGTGCAATTTCTTTCAGGATG
  GCTTGAAAACGGCTGATAAACTGAAACAGTACATAGAAAAGCTGGCTGCTGACTTATATAATATAAAGCA
  AACCCAAGATGAAGAAAAAAAACAGCTAACTGCACTCCGAGATCTAATAAAATCCTCTCTGCAACTTGAT
  CCGAAAGAAGACTCTCAGAGCCGGCAAGGTGGGTACAGCATGCATCAGCTCCAGGGCAACAAGGAGTACG
  GCAGTGAAAAGAAGGGCTTCCTGCTGAAGAAGAGTGATGGGATCCGGAAAGTGTGGCAGAGACGGAAGTG
  TGCGGTCAAGAACGGGATCCTGACCATCTCCCACGCAACTTCCAACAGGCAGCCTGCTAAGTTAAACCTC
  CTCACCTGCCAAGTGAAACCGAATGCTGAGGACAAGAAGTCTTTTGACCTGATATCACATAATCGAACAT
  ATCACTTTCAAGCAGAAGATGAGCAGGATTATATAGCGTGGATATCAGTACTGACGAATAGCAAAGAAGA
  GGCCCTAACCATGGCCTTCCGTGGTGAGCAGAGCACAGGGGAGAATAGCCTGGAGGACCTTACCAAAGCC
  ATCATCGAGGATGTACAGCGGCTCCCTGGGAATGACATCTGCTGTGACTGCGGCTCATCAGAACCCACGT
  GGCTTTCAACCAACTTGGGTATTTTGACCTGTATAGAATGTTCCGGAATCCATAGGGAAATGGGGGTTCA
  TATTTCTCGCATTCAGTCTTTGGAACTAGACAAATTAGGAACTTCCGAACTCTTGCTGGCCAAGAATGTA
  GGAAACAATAGTTTTAATGATATTATGGAAGCAAATTTACCCAGCCCTTCACCAAAGCCCACCCCTTCAA
  GTGATATGACCGTGCGTAAAGAGTACATCACTGCAAAGTATGTCGACCACCGCTTCTCCAGGAAGACCTG
  TGCCTCTTCCTCAGCAAAGCTGAACGAATTGCTTGAGGCCATCAAGTCCAGGGACTTACTTGCATTAATC
  CAAGTCTATGCAGAGGGGGTGGAGTTAATGGAGCCACTGCTGGAACCTGGGCAGGAGCTCGGGGAGACAG
  CCCTTCATCTTGCCGTCAGGACCGCAGACCAGACATCTCTCCATCTGGTTGATTTCCTTGTACAAAACTG
  TGGGAACCTGGATAAGCAAACGTCTGTGGGGAATACAGTTCTGCACTATTGCAGCATGTACGGCAAGCCG
  GAGTGTCTGAAGCTGCTGCTTAGGAGCAAGCCCACCGTGGACATCGTTAACCAGAATGGAGAAACTGCCT
  TGGATATAGCAAAGAGACTCAAAGCTACCCAGTGTGAAGATCTGCTTTCCCAGGCTAAATCTGGAAAGTT
  CAACCCTCATGTCCACGTAGAGTATGAGTGGAATCTTCGACAGGACGAGATGGATGAGAGCGATGACGAT
  CTGGATGACAAGCCAAGCCCGATCAAAAAGGAACGCTCGCCCAGACCACAGAGCTTCTGCCACTCCTCCA
  GCATCTCGCCACAGGACAAGCTGGCACTGCCAGGGTTCAGCACTCCACGGGACAAGCAGCGGCTCTCCTA
  CGGAGCCTTCACCAACCAGATCTTCGCCTCTACGAGCACAGATTTGCCCACATCACCCACCAGTGAGGCT
  CCCCCTTTGCCACCTCGGAACGCCGGGAAAGGTCCAACTGGCCCACCTTCAACACTCCCTCTAGGCACCC
  AGACCTCTAGTGGCAGCTCCACCCTATCCAAGAAGAGGCCTCCTCCCCCACCACCAGGACACAAGAGAAC
  CCTGTCTGACCCTCCCAGCCCACTACCTCACGGGCCCCCAAACAAAGGCGCAATTCCTTGGGGTAATGAT
  GTGGGCCCATTATCTTCAAGTAAGACGGCCAACAAGTTTGAGGGGCTGTCTCAGCAAGCAAGCACCAGTT
  CTGCTAAGACTGCCCTTGGCCCGAGAGTGCTTCCTAAACTACCTCAGAAAGTGGCACTAAGGAAGACGGA
  GACCAGCCATCATCTCTCCCTCGACAGAACCAACATCCCACCTGAGACTTTTCAGAAATCATCACAGTTG
  ACAGAGTTACCCCAAAAGCCACCACTTGGAGAGCTGCCCCCGAAGCCTGTGGAACTGGCCCCCAAGCCCC
  AAGTTGGAGAGCTGCCACCTAAGCCTGGAGAGCTACCCCCTAAGCCCCAATTAGGTGACCTGCCCCCCAA
  GCCACAGCTCTCAGACTTACCTCCCAAGCCACAGATGAAGGACCTGCCCCCTAAGCCGCAGCTGGGGGAT
  CTGCTGGCAAAGTCCCAGGCTGGCGATGTCTCAGCCAAGGTGCAGCCACCCTCAGAGGTCACACAGAGGT
  CACACACCGGGGATCTGTCTCCAAATGTACAGTCCAGAGATGCCATCCAGAAGCAAGCATCTGAAGACTC
  CAACGACCTCACACCCACGCTGCCAGAGACACCCGTACCACTGCCGAGAAAAATCAATACGGGGAAAAAT
  AAAGTGAGGCGGGTGAAGACCATTTATGACTGCCAGGCAGATAATGACGACGAACTCACATTTATTGAGG
  GGGAAGTGATCATTGTCACCGGGGAAGAGGACCAGGAGTGGTGGATCGGGCATATCGAAGGACAGCCTGA
  AAGGAAGGGTGTCTTTCCAGTGTCCTTTGTCCACATCCTGTCTGACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276463
• Insert Size: 3339 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276463.1, NP_001263392.1
• RefSeq Size: 6145 bp
• RefSeq ORF: 3339 bp
• Locus ID: 13196
• Cytogenetics: 15 D1
• Gene Summary: May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types. Plays a role in ciliogenesis (By similarity). Posseses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR, uses a downstream start codon, and has multiple differences in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (d) is shorter than isoform a.