Slc12a3 (NM_001205311) Mouse Tagged ORF Clone

CAT#: MR231471

Slc12a3 (myc-DDK-tagged) - Mouse solute carrier family 12, member 3 (Slc12a3), transcript variant 1

Product Images

Circular map for MR231471

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc12a3
• Synonyms: AI035291; NCC; NCCT; TSC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231471 representing NM_001205311
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGCTCCCCGTGACAGAGCTGCCTGGCGATGCTCTGTGCAGTGGGCGCTTCACCATCAGCACAC
  TGATGGGAGGCGATGAGCCCCCACCAGCTGCCTGTGACAGCAGCCAGCCCAGCCACTTAACACACGGCAG
  CACCTTATACATGCGCACCTTCGGTTACAACACCATAGACGTGGTGCCGGCCTACGAACACTATGCTAAC
  AGCGCTCTGCCCGGTGAGCCCCGGAAGGTGCGGCCCACCCTGGCTGACCTGCATTCATTCCTCAAGCAGG
  AAGGTAGCCACCTGCATGCCCTGGCCTTTGATGGACGGCAAGGTCGTGAGCTGACTGATGGACTTGTGGA
  AGATGAGACAGGCACCAACAGTGAGAAGAGCCCTGGAGAACCTGTTCGCTTCGGCTGGGTCAAGGGGGTG
  ATGATCCGTTGCATGCTCAACATCTGGGGAGTGATCCTCTACCTGCGACTCCCCTGGATTACGGCGCAGG
  CGGGCATCGTGCTGACTTGGCTCATCATCCTGCTGTCGGTCATGGTGACATCCATCACCGGCCTCTCCAT
  CTCCGCCATCTCTACCAATGGCAAGGTCAAGTCGGGTGGCACCTATTTCCTTATTTCCCGGAGTCTGGGA
  CCAGAGCTGGGAGGCTCCATTGGCCTCATCTTCGCCTTTGCCAATGCTGTGGGTGTGGCCATGCACACTG
  TGGGCTTTGCAGAAACCGTAAGGGACCTACTGCAGGAGTATGGCACACCCATCGTAGACCCCATCAATGA
  CATCCGCATCATCGGTGTGGTCACGGTCACTGTGCTGCTGGCCATCTCGCTGGCTGGGATGGAGTGGGAG
  TCCAAGGCCCAGGTGCTATTCTTTCTTGTCATCATGGTCTCCTTTGCCAACTACCTGGTGGGCACACTGA
  TCCCAGCATCTGAAGACAAAGCCTCTAAAGGCTTCTACAGCTACCATGGGGACATTTTTGTTCAGAACCT
  GGTACCCGACTGGAGAGGCATTGATGGTAGCTTCTTTGGCATGTTCTCCATCTTCTTCCCTTCGGCCACT
  GGCATTCTGGCAGGGGCCAACATCTCCGGGGACCTCAAGGATCCTGCTGTAGCCATCCCCAAGGGAACAC
  TCATGGCCATTTTCTGGACCACCATCTCCTACCTTGCCATCTCAGCTACCATCGGCTCCTGTGTGGTGAG
  AGATGCCTCTGGGGATGTGAACGACACCATGACTCCTGGTCCAGGCCCCTGCGAAGGGCTAGCCTGTGGC
  TATGGCTGGAACTTCACAGAATGCTCCCAGCAGCGCAGTTGCCGCTATGGCCTCATCAACTACTACCAGA
  CCATGAGCATGGTGTCTGCCTTCGCACCCCTGATCACAGCCGGCATCTTTGGCGCCACCCTGTCTTCTGC
  CTTGGCCTGTCTTGTCTCGGCTGCTAAGGTCTTCCAGTGCCTGTGTGAAGACCAGCTGTACCCTCTGATT
  GGCTTTTTTGGCAAAGGCTATGGCAAGAACAGGGAGCCTGTGCGTGGCTACCTGCTGGCCTATGCCATTG
  CTGTGGCCTTCATCATCATTGCTGAACTTAACACCATCGCCCCCATCATTTCCAACTTCTTCCTCTGCTC
  CTACGCCCTCATCAATTTCAGCTGCTTCCATGCCTCCATCACCAACTCACCTGGATGGAGACCCTCCTTC
  CGATACTACAGCAAGTGGGCGGCGCTGTTTGGGGCAGTGATCTCGGTGGTCATCATGTTCCTGCTTACCT
  GGTGGGCTGCTCTCATTGCCATCGGAGTCGTCCTCTTCCTCCTGCTCTATGTGATCTACAAGAAGCCAGA
  GGTGAACTGGGGTTCCTCAGTACAGGCTGGCTCCTACAATCTGGCCCTGAGCTACTCCGTGGGCCTCAAC
  GAGGTGGAAGACCACATCAAGAACTACCGCCCCCAGTGCCTGGTGCTTACAGGGCCCCCCAACTTCCGCC
  CAGCCCTTGTGGACTTTGTGAGCACATTTACCCAGAACCTAAGCCTAATGATTTGTGGTCATGTGCTCAT
  TGGACCTGGCAAGCAGAGAGTGCCCGAGCTCCGGCTCATCGCCAGTGGACACACCAAGTGGCTGAACAAG
  AGGAAGATCAAGGCCTTCTACTCTGACGTCATCGCTGAGGACTTGCGCAGTGGCGTCCAGATTCTCATGC
  AGGCCTCAGGGCTTGGGAGAATGAAGCCGAACATTCTGGTGGTTGGATTCAAGAGGAACTGGCAGTCTGC
  CCACCCAGCCACAGTGGAGGACTACATTGGTGTCCTGCATGATGCCTTTGACTTCAACTACGGGGTTTGT
  GTCATGAGGATGCGCGAGGGGCTCAATGTCTCCGAGGCTCTGCAGACCCACACAACCCCAGAGGCATTGA
  TACAAGAGGAGCAGGCTAGCACCATCTTCCAGTCGGAGCAAGGCAAGAAGACCATTGACATCTACTGGCT
  GTTCGATGATGGAGGTCTCACCCTCCTCATCCCCTATCTCCTGCACCGCAAGAAGAGATGGGGCAAATGC
  AAGATCCGAGTGTTTGTTGGGGGCCAGATCAACAGGATGGACGAAGAGAGAAAGGCGATCATCTCTCTGT
  TGAGCAAGTTCAGACTGGGATTCCATGAAGTTCACGTCCTTCCCGACATCAACCAGAAGCCCCAGGCCGA
  GCACACCAAGCGGTTTGAGGACATGATTGCACCCTTCCGCCTCAATGATGGCTTCAAGGACGAGGCCACC
  GTCACTGAGATGAGGAGGGACTGTCCCTGGAAGATCTCAGATGAAGAGATTAACAAGAACAGAATTAAGT
  CCCTTCGGCAGGTGAGACTGAGTGAGATACTGCTGGATTACTCTCGGGATGCTGCTCTGATCATCCTTAC
  CCTGCCCATTGGAAGGAAGGGGAAGTGCCCCAGTTCGCTGTACATGGCCTGGCTGGAGACCCTGTCCCAG
  GACCTCAGACCTCCCGTCCTCCTCATCCGAGGCAACCAGGAGAATGTGCTCACCTTTTACTGCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231471 representing NM_001205311
  Red=Cloning site Green=Tags(s)
  
  MAELPVTELPGDALCSGRFTISTLMGGDEPPPAACDSSQPSHLTHGSTLYMRTFGYNTIDVVPAYEHYAN
  SALPGEPRKVRPTLADLHSFLKQEGSHLHALAFDGRQGRELTDGLVEDETGTNSEKSPGEPVRFGWVKGV
  MIRCMLNIWGVILYLRLPWITAQAGIVLTWLIILLSVMVTSITGLSISAISTNGKVKSGGTYFLISRSLG
  PELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGTPIVDPINDIRIIGVVTVTVLLAISLAGMEWE
  SKAQVLFFLVIMVSFANYLVGTLIPASEDKASKGFYSYHGDIFVQNLVPDWRGIDGSFFGMFSIFFPSAT
  GILAGANISGDLKDPAVAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGDVNDTMTPGPGPCEGLACG
  YGWNFTECSQQRSCRYGLINYYQTMSMVSAFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLI
  GFFGKGYGKNREPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSF
  RYYSKWAALFGAVISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLN
  EVEDHIKNYRPQCLVLTGPPNFRPALVDFVSTFTQNLSLMICGHVLIGPGKQRVPELRLIASGHTKWLNK
  RKIKAFYSDVIAEDLRSGVQILMQASGLGRMKPNILVVGFKRNWQSAHPATVEDYIGVLHDAFDFNYGVC
  VMRMREGLNVSEALQTHTTPEALIQEEQASTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLHRKKRWGKC
  KIRVFVGGQINRMDEERKAIISLLSKFRLGFHEVHVLPDINQKPQAEHTKRFEDMIAPFRLNDGFKDEAT
  VTEMRRDCPWKISDEEINKNRIKSLRQVRLSEILLDYSRDAALIILTLPIGRKGKCPSSLYMAWLETLSQ
  DLRPPVLLIRGNQENVLTFYCQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001205311
• ORF Size: 3006 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205311.1, NP_001192240.1
• RefSeq Size: 3300 bp
• RefSeq ORF: 3009 bp
• Locus ID: 20497
• UniProt ID: P59158
• Cytogenetics: 8 46.46 cM
• MW: 111.1 kDa
• Gene Summary: Electroneutral sodium and chloride ion cotransporter. In kidney distal convoluted tubules, key mediator of sodium and chloride reabsorption (By similarity). Receptor for the proinflammatory cytokine IL18. Contributes to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2. May act either independently of IL18R1, or in a complex with IL18R1 (PubMed:26099046).[UniProtKB/Swiss-Prot Function]