Slc12a3 (NM_001205311) Mouse Untagged Clone

CAT#: MC229260

Slc12a3 (untagged) - Mouse solute carrier family 12, member 3 (Slc12a3), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slc12a3
• Synonyms: AI035291; NCC; NCCT; TSC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229260 representing NM_001205311
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGCTCCCCGTGACAGAGCTGCCTGGCGATGCTCTGTGCAGTGGGCGCTTCACCATCAGCACAC
  TGATGGGAGGCGATGAGCCCCCACCAGCTGCCTGTGACAGCAGCCAGCCCAGCCACTTAACACACGGCAG
  CACCTTATACATGCGCACCTTCGGTTACAACACCATAGACGTGGTGCCGGCCTACGAACACTATGCTAAC
  AGCGCTCTGCCCGGTGAGCCCCGGAAGGTGCGGCCCACCCTGGCTGACCTGCATTCATTCCTCAAGCAGG
  AAGGTAGCCACCTGCATGCCCTGGCCTTTGATGGACGGCAAGGTCGTGAGCTGACTGATGGACTTGTGGA
  AGATGAGACAGGCACCAACAGTGAGAAGAGCCCTGGAGAACCTGTTCGCTTCGGCTGGGTCAAGGGGGTG
  ATGATCCGTTGCATGCTCAACATCTGGGGAGTGATCCTCTACCTGCGACTCCCCTGGATTACGGCGCAGG
  CGGGCATCGTGCTGACTTGGCTCATCATCCTGCTGTCGGTCATGGTGACATCCATCACCGGCCTCTCCAT
  CTCCGCCATCTCTACCAATGGCAAGGTCAAGTCGGGTGGCACCTATTTCCTTATTTCCCGGAGTCTGGGA
  CCAGAGCTGGGAGGCTCCATTGGCCTCATCTTCGCCTTTGCCAATGCTGTGGGTGTGGCCATGCACACTG
  TGGGCTTTGCAGAAACCGTAAGGGACCTACTGCAGGAGTATGGCACACCCATCGTAGACCCCATCAATGA
  CATCCGCATCATCGGTGTGGTCACGGTCACTGTGCTGCTGGCCATCTCGCTGGCTGGGATGGAGTGGGAG
  TCCAAGGCCCAGGTGCTATTCTTTCTTGTCATCATGGTCTCCTTTGCCAACTACCTGGTGGGCACACTGA
  TCCCAGCATCTGAAGACAAAGCCTCTAAAGGCTTCTACAGCTACCATGGGGACATTTTTGTTCAGAACCT
  GGTACCCGACTGGAGAGGCATTGATGGTAGCTTCTTTGGCATGTTCTCCATCTTCTTCCCTTCGGCCACT
  GGCATTCTGGCAGGGGCCAACATCTCCGGGGACCTCAAGGATCCTGCTGTAGCCATCCCCAAGGGAACAC
  TCATGGCCATTTTCTGGACCACCATCTCCTACCTTGCCATCTCAGCTACCATCGGCTCCTGTGTGGTGAG
  AGATGCCTCTGGGGATGTGAACGACACCATGACTCCTGGTCCAGGCCCCTGCGAAGGGCTAGCCTGTGGC
  TATGGCTGGAACTTCACAGAATGCTCCCAGCAGCGCAGTTGCCGCTATGGCCTCATCAACTACTACCAGA
  CCATGAGCATGGTGTCTGCCTTCGCACCCCTGATCACAGCCGGCATCTTTGGCGCCACCCTGTCTTCTGC
  CTTGGCCTGTCTTGTCTCGGCTGCTAAGGTCTTCCAGTGCCTGTGTGAAGACCAGCTGTACCCTCTGATT
  GGCTTTTTTGGCAAAGGCTATGGCAAGAACAGGGAGCCTGTGCGTGGCTACCTGCTGGCCTATGCCATTG
  CTGTGGCCTTCATCATCATTGCTGAACTTAACACCATCGCCCCCATCATTTCCAACTTCTTCCTCTGCTC
  CTACGCCCTCATCAATTTCAGCTGCTTCCATGCCTCCATCACCAACTCACCTGGATGGAGACCCTCCTTC
  CGATACTACAGCAAGTGGGCGGCGCTGTTTGGGGCAGTGATCTCGGTGGTCATCATGTTCCTGCTTACCT
  GGTGGGCTGCTCTCATTGCCATCGGAGTCGTCCTCTTCCTCCTGCTCTATGTGATCTACAAGAAGCCAGA
  GGTGAACTGGGGTTCCTCAGTACAGGCTGGCTCCTACAATCTGGCCCTGAGCTACTCCGTGGGCCTCAAC
  GAGGTGGAAGACCACATCAAGAACTACCGCCCCCAGTGCCTGGTGCTTACAGGGCCCCCCAACTTCCGCC
  CAGCCCTTGTGGACTTTGTGAGCACATTTACCCAGAACCTAAGCCTAATGATTTGTGGTCATGTGCTCAT
  TGGACCTGGCAAGCAGAGAGTGCCCGAGCTCCGGCTCATCGCCAGTGGACACACCAAGTGGCTGAACAAG
  AGGAAGATCAAGGCCTTCTACTCTGACGTCATCGCTGAGGACTTGCGCAGTGGCGTCCAGATTCTCATGC
  AGGCCTCAGGGCTTGGGAGAATGAAGCCGAACATTCTGGTGGTTGGATTCAAGAGGAACTGGCAGTCTGC
  CCACCCAGCCACAGTGGAGGACTACATTGGTGTCCTGCATGATGCCTTTGACTTCAACTACGGGGTTTGT
  GTCATGAGGATGCGCGAGGGGCTCAATGTCTCCGAGGCTCTGCAGACCCACACAACCCCAGAGGCATTGA
  TACAAGAGGAGCAGGCTAGCACCATCTTCCAGTCGGAGCAAGGCAAGAAGACCATTGACATCTACTGGCT
  GTTCGATGATGGAGGTCTCACCCTCCTCATCCCCTATCTCCTGCACCGCAAGAAGAGATGGGGCAAATGC
  AAGATCCGAGTGTTTGTTGGGGGCCAGATCAACAGGATGGACGAAGAGAGAAAGGCGATCATCTCTCTGT
  TGAGCAAGTTCAGACTGGGATTCCATGAAGTTCACGTCCTTCCCGACATCAACCAGAAGCCCCAGGCCGA
  GCACACCAAGCGGTTTGAGGACATGATTGCACCCTTCCGCCTCAATGATGGCTTCAAGGACGAGGCCACC
  GTCACTGAGATGAGGAGGGACTGTCCCTGGAAGATCTCAGATGAAGAGATTAACAAGAACAGAATTAAGT
  CCCTTCGGCAGGTGAGACTGAGTGAGATACTGCTGGATTACTCTCGGGATGCTGCTCTGATCATCCTTAC
  CCTGCCCATTGGAAGGAAGGGGAAGTGCCCCAGTTCGCTGTACATGGCCTGGCTGGAGACCCTGTCCCAG
  GACCTCAGACCTCCCGTCCTCCTCATCCGAGGCAACCAGGAGAATGTGCTCACCTTTTACTGCCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001205311
• Insert Size: 3009 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205311.1, NP_001192240.1
• RefSeq Size: 3300 bp
• RefSeq ORF: 3009 bp
• Locus ID: 20497
• UniProt ID: P59158
• Cytogenetics: 8 46.46 cM
• Gene Summary: Electroneutral sodium and chloride ion cotransporter. In kidney distal convoluted tubules, key mediator of sodium and chloride reabsorption (By similarity). Receptor for the proinflammatory cytokine IL18. Contributes to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2. May act either independently of IL18R1, or in a complex with IL18R1 (PubMed:26099046).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.