Usp15 (NM_001301628) Mouse Tagged ORF Clone

CAT#: MR231419

Usp15 (myc-DDK-tagged) - Mouse ubiquitin specific peptidase 15 (Usp15), transcript variant 2

Product Images

Circular map for MR231419

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Usp15
• Synonyms: 4921514G19Rik; AI327321; E430033I05Rik; Gcap; Gcap18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231419 representing NM_001301628
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAAGGCGGAGCGGCGGACCTGGACACCCAGCGTTCTGACATCGCGACGCTGCTCAAAACCTCGC
  TCCGGAAAGGGGACACCTGGTATCTAGTAGATAGTCGGTGGTTCAAACAGTGGAAAAAATATGTTGGCTT
  TGACAGCTGGGACAAATACCAGATGGGAGATCAAAATGTCTATCCTGGACCCATCGATAACTCTGGACTT
  CTCAAAGATGGCGACGCGCAGTCACTTAAGGAGCACCTTATTGATGAGTTGGATTACATACTCTTGCCAA
  CTGAGGGCTGGAATAAACTTGTCAGCTGGTACACACTGATGGAGGGCCAGGAGCCAATAGCAAGAAAGGT
  GGTAGAACAGGGTATGTTTGTAAAGCACTGCAAAGTAGAAGTCTACCTCACAGAACTGAAGCTCTGTGAG
  AACGGGAACATGAACAATGTTGTAACTCGGAGGTTTAGCAAAGCTGACACAATAGATACGATTGAGAAGG
  AAATAAGAAAAATCTTCAATATTCCAGATGAGAAGGAGGCCAGACTGTGGAACAAGTATATGAGCAACAC
  ATTTGAACCACTGAATAAGCCAGACAGCACCATCCAGGATGCCGGCCTATACCAAGGACAGGTGTTAGTG
  ATAGAGCAAAAAAATGAAGATGGAACGTGGCCCAGGGGTCCCTCTACTCCTAATGTGAAAAACTCAAATT
  ATTGTCTCCCGTCATATACTGCTTATAAGAACTATGATTATTCAGAACCTGGAAGAAACAATGAACAGCC
  AGGCCTGTGTGGCTTAAGTAACTTGGGAAATACGTGTTTCATGAACTCAGCCATTCAGTGTTTGAGCAAC
  ACACCTCCACTTACTGAATATTTCCTCAATGATAAGTACCAAGAAGAGCTGAATTTTGACAATCCCTTAG
  GAATGAGAGGTGAAATAGCTAAATCATACGCTGAACTCATCAAGCAGATGTGGTCTGGAAAGTTTAGCTA
  CGTCACTCCAAGAGCATTCAAGACACAGGTAGGGCGCTTCGCACCTCAGTTCTCTGGATATCAACAGCAG
  GACTGCCAAGAACTACTGGCTTTCCTGTTGGATGGACTGCACGAGGACCTGAATAGAATTAGGAAAAAAC
  CCTATATCCAGTTAAAAGATGCTGATGGCAGGCCAGACAAGGTGGTTGCTGAAGAAGCCTGGGAGAACCA
  TTTAAAGCGAAATGATTCCATCATAGTAGACATATTCCATGGCCTTTTCAAGTCTACGTTAGTGTGTCCT
  GAGTGTGCTAAGATCTCGGTGACCTTTGACCCTTTCTGTTACTTGACTCTTCCATTGCCTATGAAAAAAG
  AACGTAGCTTGGAAGTTTATTTAGTTAGAATGGATCCACTTGCTAAACCTATGCAATACAAAGTTATTGT
  GCCCAAAATTGGAAATATACTAGATCTTTGTACAGCGCTGTCTGCTTTGTCGGGAGTACCTGCAGATAAG
  ATGATAGTCACTGACATATACAATCATAGATTTCACAGGATATTTGCCGTGGATGAAAACCTGAGTAGTA
  TTATGGAGCGGGATGATATTTACGTGTTTGAAATTAACATCAACAGGGCAGAAGACACAGAGCATGTAGT
  TATCCCTGTCTGCCTAAGAGAAAAATTCAGACACTCAAGTTACACTCACCACACTGGCTCTTCACTGTTC
  GGCCAGCCTTTTCTTATGGCTATACCACGAAACAATACTGAAGATAAACTCTATAATCTTCTACTCTTGA
  GAATGTGCCGGTATGTCAAAATGTCTACTGAAACTGAGGAAACAGATGGACACCTACGTTGCTGTGAGGA
  CCAGAATATTAATGGGAATGGGCCAAATGGCCTACATGAAGAGGGTTCACCAAGTGAGATGGAAACCGAC
  GAGCCAGATGACGAGTCCAGCCAGGATCAAGAGCTTCCCTCAGAGAACGAGAACAGTCAGTCTGAAGATT
  CAGTCGGAGGAGATAACGATTCTGAAAATGGATTGTGCACTGAAGAAACATGCAAAGGTCAACTTACGGG
  ACACAAAAAGCGCTTGTTTACATTCCAGTTCAACAACTTAGGCAACAATGACATCAACTACATCAAAGAC
  GACACCAGTCACATCCGATTCGATGACAGGCAGCTTCGGCTGGACGAAAGATCTTTTCTCGCTTTGGACT
  GGGACCCTGACTTGAAGAAGAGATACTTTGATGAGAATGCTGCAGAGGATTTTGAAAAACATGAAAGTGT
  GGAATACAAACCTCCAAAAAGGCCCTTTGTGAAGTTAAAAGACTGCATTGAGCTCTTCACCACCAAGGAG
  AAGCTGGGTGCTGAGGACCCCTGGTATTGTCCAAATTGTAAAGAGCATCAGCAAGCCACAAAGAAATTAG
  ATCTGTGGTCCCTGCCTCCAGTTCTTGTGGTGCATCTCAAGAGATTCTCCTATAGTCGCTACATGAGAGA
  CAAGCTAGATACATTAGTCGATTTTCCCATCAGTGACTTGGATATGTCAGAATTCCTAATTAACCCAAAT
  GCAGGTCCTTGCCGCTATAATTTGATTGCTGTTTCCAACCACTATGGAGGGATGGGAGGAGGACATTATA
  CTGCTTTTGCAAAAAACAAAGATGATGGGAAGTGGTACTATTTCGATGACAGTAGCGTCTCCACTGCATC
  TGAAGACCAAATCGTGTCCAAGGCAGCGTACGTGCTCTTCTACCAGAGACAGGACACTTTCAGTGGGACT
  GGCTTCTTCCCTCTTGACCGAGAAACTAAAGGTGCTTCAGCTGCCACAGGTATCCCCCTGGAAAGTGACG
  AAGACAGCAATGATAATGACAATGACCTAGAAAATGAAAACTGTATGCACACTAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231419 representing NM_001301628
  Red=Cloning site Green=Tags(s)
  
  MAEGGAADLDTQRSDIATLLKTSLRKGDTWYLVDSRWFKQWKKYVGFDSWDKYQMGDQNVYPGPIDNSGL
  LKDGDAQSLKEHLIDELDYILLPTEGWNKLVSWYTLMEGQEPIARKVVEQGMFVKHCKVEVYLTELKLCE
  NGNMNNVVTRRFSKADTIDTIEKEIRKIFNIPDEKEARLWNKYMSNTFEPLNKPDSTIQDAGLYQGQVLV
  IEQKNEDGTWPRGPSTPNVKNSNYCLPSYTAYKNYDYSEPGRNNEQPGLCGLSNLGNTCFMNSAIQCLSN
  TPPLTEYFLNDKYQEELNFDNPLGMRGEIAKSYAELIKQMWSGKFSYVTPRAFKTQVGRFAPQFSGYQQQ
  DCQELLAFLLDGLHEDLNRIRKKPYIQLKDADGRPDKVVAEEAWENHLKRNDSIIVDIFHGLFKSTLVCP
  ECAKISVTFDPFCYLTLPLPMKKERSLEVYLVRMDPLAKPMQYKVIVPKIGNILDLCTALSALSGVPADK
  MIVTDIYNHRFHRIFAVDENLSSIMERDDIYVFEININRAEDTEHVVIPVCLREKFRHSSYTHHTGSSLF
  GQPFLMAIPRNNTEDKLYNLLLLRMCRYVKMSTETEETDGHLRCCEDQNINGNGPNGLHEEGSPSEMETD
  EPDDESSQDQELPSENENSQSEDSVGGDNDSENGLCTEETCKGQLTGHKKRLFTFQFNNLGNNDINYIKD
  DTSHIRFDDRQLRLDERSFLALDWDPDLKKRYFDENAAEDFEKHESVEYKPPKRPFVKLKDCIELFTTKE
  KLGAEDPWYCPNCKEHQQATKKLDLWSLPPVLVVHLKRFSYSRYMRDKLDTLVDFPISDLDMSEFLINPN
  AGPCRYNLIAVSNHYGGMGGGHYTAFAKNKDDGKWYYFDDSSVSTASEDQIVSKAAYVLFYQRQDTFSGT
  GFFPLDRETKGASAATGIPLESDEDSNDNDNDLENENCMHTN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301628
• ORF Size: 2856 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301628.1, NP_001288557.1
• RefSeq Size: 11477 bp
• RefSeq ORF: 2859 bp
• Locus ID: 14479
• UniProt ID: Q8R5H1
• Cytogenetics: 10 D2
• MW: 109.7 kDa
• Gene Summary: The protein encoded by this gene is a member of the large ubiquitin specific protease (Usp) family of proteins. These proteins are known to cleave ubiquitin, and contain a conserved cysteine residue (Cys box) and two conserved histidine residues (His box) that are thought to form part of the active site of the protease. This protein has been shown to cleave both the ubiquitin-proline and the ubiquitin-methionine bonds in vitro. This protein is thought to regulate many cellular processes through its deubiquitination activity, including the transforming growth factor beta (TGF-beta) pathway. Cardiac-specific overexpression of the human ortholog of this gene in mice causes enlargement of the heart that is more pronounced in the atrium than in the ventricle. This gene has two pseudogenes on chromosome 14. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms.[provided by RefSeq, Aug 2014]