Lig1 (NM_001199310) Mouse Tagged ORF Clone

CAT#: MR231400

Lig1 (myc-DDK-tagged) - Mouse ligase I, DNA, ATP-dependent (Lig1), transcript variant 3

Product Images

Circular map for MR231400

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lig1
• Synonyms: AL033288; LigI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231400 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAAAAAAAGAGCAAGAGAGGAAAGGAGAGACTTCTGCTGCCAACATGCAGAGAAGTATTATGTCAT
  TTTTTCAACCCACAAAAGAAGGTAAAGCGAAGAAGCCAGAGAAGGAGACACCCAGCAGCATCAGAGAGAA
  GGAACCCCCTCCAAAGGTGGCGCTGAAGGAGAGGAATCAAGTGGTGCCCGAGAGTGATTCTCCAGTGAAG
  AGGACAGGAAGGAAGGTAGCCCAGGTTCTGAGCTGTGAAGGGGAGGACGAAGATGAAGCCCCTGGCACCC
  CCAAAGTCCAGAAGCCTGTGTCAGACTCTGAACAGAGCTCTCCTCCCAGCCCTGACACATGTCCTGAGAA
  CAGTCCTGTCTTCAACTGCAGCTCCCCCATGGACATCTCCCCATCAGGATTCCCAAAGCGTCGAACTGCG
  CGGAAGCAGCTCCCCAAACGGACAATTCAAGACACTCTTGAGGAGCAGAATGAGGACAAAACCAAAACAG
  CTAAGAAAAGGAAGAAAGAAGAAGAGACCCCAAAAGAAAGCCTCGCAGAGGCTGAAGACGTAAAACAGAA
  GGAAGAAAAGGAGGGGGACCAGCTCATAGTCCCCTCTGAGCCCACAAAGTCCCCTGAGTCAGTAACCCTG
  ACAAAGACAGAGAACATTCCAGTGTGTAAGGCAGGAGTGAAACTGAAGCCTCAGGAAGAGGAACAGAGCA
  AACCTCCTGCTAGAGGTGCCAAGACACTGAGCAGTTTCTTCACACCCCGAAAACCAGCAGTGAAAACTGA
  AGTCAAACAAGAAGAGTCAGGCACTCTAAGGAAGGAAGAGACCAAGGGAACCCTGGATCCAGCAAATTAT
  AATCCTTCCAAGAATAACTATCATCCCATTGAAGATGCCTGCTGGAAACATGGCCAGAAGGTCCCTTTTC
  TCGCCGTGGCCCGGACATTTGAGAAGATTGAGGAGGTTTCTGCTAGGCTCAAGATGGTGGAGACATTGAG
  CAACCTGCTGCGCTCCGTGGTGGCCCTGTCCCCTCCAGACTTGCTCCCTGTTCTCTATCTCAGCCTCAAC
  CGCCTCGGGCCACCTCAGCAGGGACTAGAGCTGGGTGTTGGTGATGGTGTCCTCCTCAAGGCCGTTGCCC
  AGGCCACAGGTCGTCAGCTGGAGTCCATCCGGGCTGAGGTAGCTGAGAAGGGGGATGTGGGGCTGGTGGC
  CGAGAACAGCCGCAGCACTCAGAGACTCATGCTGCCTCCACCTCCACTTACTATTTCTGGAGTCTTCACC
  AAATTCTGTGACATTGCCCGGCTCACTGGCAGTGCTTCCATGGCCAAGAAGATGGACATTATCAAGGGCC
  TTTTTGTTGCCTGCCGCCACTCAGAAGCCAGGTACATTGCCAGGTCCCTAAGCGGGCGGCTGCGCCTTGG
  CCTAGCTGAGCAGTCGGTACTGGCTGCTCTTGCCCAGGCTGTGAGCCTCACACCCCCTGGCCAAGAATTT
  CCCACGGCGGTGGTGGATGCTGGGAAGGGCAAGACAGCAGAGGCCAGAAAGATGTGGTTGGAAGAACAAG
  GCATGATCTTGAAGCAGACCTTCTGTGAGGTTCCTGACCTGGATCGAATCATCCCTGTGCTGCTGGAACA
  TGGCCTGGAACGTCTCCCAGAGCACTGCAAGCTGAGCCCAGGGGTCCCTCTTAAACCAATGCTGGCTCAT
  CCCACTCGGGGTGTCAGTGAGGTCCTAAAACGCTTTGAGGAGGTCGACTTCACCTGTGAATATAAATACG
  ATGGGCAGCGAGCACAGATACATGTTCTGGAAGGTGGAGAAGTGAAGATCTTCAGCAGGAACCAGGAAGA
  CAACACAGGAAAGTATCCTGACATTATCAGCCGCATCCCCAAGATTAAACACCCCTCAGTCACATCTTTC
  ATTCTGGACACTGAGGCTGTGGCCTGGGACCGGGAAAAGAAGCAGATCCAACCATTCCAAGTACTCACCA
  CGCGCAAGCGCAAGGAGGTTGATGCATCGGAGATACAGGTGCAAGTGTGTCTGTATGCTTTTGACCTCAT
  CTACCTCAACGGAGAGTCCCTGGTTCGCCAGCCCCTGTCTCGACGCCGGCAGCTGCTCCGTGAGAACTTC
  GTGGAGACAGAGGGTGAGTTTGTCTTCACCACCTCTTTGGACACCAAGGACACCGAGCAGATTGCTGAGT
  TCTTGGAGCAGTCAGTGAAGGACTCCTGTGAGGGCCTGATGGTGAAGACCTTGGATGTTGATGCCACTTA
  TGAGATTGCCAAGAGGTCACACAACTGGCTCAAGCTGAAGAAGGACTACCTTGACGGTGTGGGTGACACT
  CTGGACCTTGTGGTGATTGGTGCCTACCTGGGCCGGGGGAAGCGGGCTGGCCGGTATGGAGGCTTCCTGT
  TGGCTGCCTACGATGAGGAGAGTGAAGAGCTTCAGGCTATATGCAAGCTGGGAACTGGATTCAGCGATGA
  GGAGCTGGAGGAACACCACCAGAGCCTGCAGGCCCTGGTATTGCCTACCCCACGCCCCTATGTGAGGATT
  GATGGGGCAGTGGCCCCAGACCATTGGCTGGACCCAAGCATTGTGTGGGAGGTGAAGTGTGCTGATCTTT
  CCCTGTCCCCCATCTACCCTGCTGCACGGGGCCTGGTGGACAAAGAAAAAGGGATCTCTCTTCGTTTTCC
  TCGGTTCATTCGTGTCCGTAAAGACAAGCAGCCAGAGCAGGCCACTACCAGTAACCAGGTTGCCTCTTTG
  TACCGGAAGCAGAGTCAGATTCAGAACCAACAAAGCTCAGACTTGGACTCCGACGTTGAAGACTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231400 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRKKEQERKGETSAANMQRSIMSFFQPTKEGKAKKPEKETPSSIREKEPPPKVALKERNQVVPESDSPVK
  RTGRKVAQVLSCEGEDEDEAPGTPKVQKPVSDSEQSSPPSPDTCPENSPVFNCSSPMDISPSGFPKRRTA
  RKQLPKRTIQDTLEEQNEDKTKTAKKRKKEEETPKESLAEAEDVKQKEEKEGDQLIVPSEPTKSPESVTL
  TKTENIPVCKAGVKLKPQEEEQSKPPARGAKTLSSFFTPRKPAVKTEVKQEESGTLRKEETKGTLDPANY
  NPSKNNYHPIEDACWKHGQKVPFLAVARTFEKIEEVSARLKMVETLSNLLRSVVALSPPDLLPVLYLSLN
  RLGPPQQGLELGVGDGVLLKAVAQATGRQLESIRAEVAEKGDVGLVAENSRSTQRLMLPPPPLTISGVFT
  KFCDIARLTGSASMAKKMDIIKGLFVACRHSEARYIARSLSGRLRLGLAEQSVLAALAQAVSLTPPGQEF
  PTAVVDAGKGKTAEARKMWLEEQGMILKQTFCEVPDLDRIIPVLLEHGLERLPEHCKLSPGVPLKPMLAH
  PTRGVSEVLKRFEEVDFTCEYKYDGQRAQIHVLEGGEVKIFSRNQEDNTGKYPDIISRIPKIKHPSVTSF
  ILDTEAVAWDREKKQIQPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYLNGESLVRQPLSRRRQLLRENF
  VETEGEFVFTTSLDTKDTEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDT
  LDLVVIGAYLGRGKRAGRYGGFLLAAYDEESEELQAICKLGTGFSDEELEEHHQSLQALVLPTPRPYVRI
  DGAVAPDHWLDPSIVWEVKCADLSLSPIYPAARGLVDKEKGISLRFPRFIRVRKDKQPEQATTSNQVASL
  YRKQSQIQNQQSSDLDSDVEDY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199310
• ORF Size: 2796 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199310.1, NP_001186239.1
• RefSeq Size: 3194 bp
• RefSeq ORF: 2799 bp
• Locus ID: 16881
• Cytogenetics: 7 7.82 cM
• MW: 104.1 kDa
• Gene Summary: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.[UniProtKB/Swiss-Prot Function]