Lig1 (NM_001199310) Mouse Untagged Clone

CAT#: MC229189

Lig1 (untagged) - Mouse ligase I, DNA, ATP-dependent (Lig1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lig1
• Synonyms: AL033288; LigI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229189 representing NM_001199310
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAAAAAAAGAGCAAGAGAGGAAAGGAGAGACTTCTGCTGCCAACATGCAGAGAAGTATTATGTCAT
  TTTTTCAACCCACAAAAGAAGGTAAAGCGAAGAAGCCAGAGAAGGAGACACCCAGCAGCATCAGAGAGAA
  GGAACCCCCTCCAAAGGTGGCGCTGAAGGAGAGGAATCAAGTGGTGCCCGAGAGTGATTCTCCAGTGAAG
  AGGACAGGAAGGAAGGTAGCCCAGGTTCTGAGCTGTGAAGGGGAGGACGAAGATGAAGCCCCTGGCACCC
  CCAAAGTCCAGAAGCCTGTGTCAGACTCTGAACAGAGCTCTCCTCCCAGCCCTGACACATGTCCTGAGAA
  CAGTCCTGTCTTCAACTGCAGCTCCCCCATGGACATCTCCCCATCAGGATTCCCAAAGCGTCGAACTGCG
  CGGAAGCAGCTCCCCAAACGGACAATTCAAGACACTCTTGAGGAGCAGAATGAGGACAAAACCAAAACAG
  CTAAGAAAAGGAAGAAAGAAGAAGAGACCCCAAAAGAAAGCCTCGCAGAGGCTGAAGACGTAAAACAGAA
  GGAAGAAAAGGAGGGGGACCAGCTCATAGTCCCCTCTGAGCCCACAAAGTCCCCTGAGTCAGTAACCCTG
  ACAAAGACAGAGAACATTCCAGTGTGTAAGGCAGGAGTGAAACTGAAGCCTCAGGAAGAGGAACAGAGCA
  AACCTCCTGCTAGAGGTGCCAAGACACTGAGCAGTTTCTTCACACCCCGAAAACCAGCAGTGAAAACTGA
  AGTCAAACAAGAAGAGTCAGGCACTCTAAGGAAGGAAGAGACCAAGGGAACCCTGGATCCAGCAAATTAT
  AATCCTTCCAAGAATAACTATCATCCCATTGAAGATGCCTGCTGGAAACATGGCCAGAAGGTCCCTTTTC
  TCGCCGTGGCCCGGACATTTGAGAAGATTGAGGAGGTTTCTGCTAGGCTCAAGATGGTGGAGACATTGAG
  CAACCTGCTGCGCTCCGTGGTGGCCCTGTCCCCTCCAGACTTGCTCCCTGTTCTCTATCTCAGCCTCAAC
  CGCCTCGGGCCACCTCAGCAGGGACTAGAGCTGGGTGTTGGTGATGGTGTCCTCCTCAAGGCCGTTGCCC
  AGGCCACAGGTCGTCAGCTGGAGTCCATCCGGGCTGAGGTAGCTGAGAAGGGGGATGTGGGGCTGGTGGC
  CGAGAACAGCCGCAGCACTCAGAGACTCATGCTGCCTCCACCTCCACTTACTATTTCTGGAGTCTTCACC
  AAATTCTGTGACATTGCCCGGCTCACTGGCAGTGCTTCCATGGCCAAGAAGATGGACATTATCAAGGGCC
  TTTTTGTTGCCTGCCGCCACTCAGAAGCCAGGTACATTGCCAGGTCCCTAAGCGGGCGGCTGCGCCTTGG
  CCTAGCTGAGCAGTCGGTACTGGCTGCTCTTGCCCAGGCTGTGAGCCTCACACCCCCTGGCCAAGAATTT
  CCCACGGCGGTGGTGGATGCTGGGAAGGGCAAGACAGCAGAGGCCAGAAAGATGTGGTTGGAAGAACAAG
  GCATGATCTTGAAGCAGACCTTCTGTGAGGTTCCTGACCTGGATCGAATCATCCCTGTGCTGCTGGAACA
  TGGCCTGGAACGTCTCCCAGAGCACTGCAAGCTGAGCCCAGGGGTCCCTCTTAAACCAATGCTGGCTCAT
  CCCACTCGGGGTGTCAGTGAGGTCCTAAAACGCTTTGAGGAGGTCGACTTCACCTGTGAATATAAATACG
  ATGGGCAGCGAGCACAGATACATGTTCTGGAAGGTGGAGAAGTGAAGATCTTCAGCAGGAACCAGGAAGA
  CAACACAGGAAAGTATCCTGACATTATCAGCCGCATCCCCAAGATTAAACACCCCTCAGTCACATCTTTC
  ATTCTGGACACTGAGGCTGTGGCCTGGGACCGGGAAAAGAAGCAGATCCAACCATTCCAAGTACTCACCA
  CGCGCAAGCGCAAGGAGGTTGATGCATCGGAGATACAGGTGCAAGTGTGTCTGTATGCTTTTGACCTCAT
  CTACCTCAACGGAGAGTCCCTGGTTCGCCAGCCCCTGTCTCGACGCCGGCAGCTGCTCCGTGAGAACTTC
  GTGGAGACAGAGGGTGAGTTTGTCTTCACCACCTCTTTGGACACCAAGGACACCGAGCAGATTGCTGAGT
  TCTTGGAGCAGTCAGTGAAGGACTCCTGTGAGGGCCTGATGGTGAAGACCTTGGATGTTGATGCCACTTA
  TGAGATTGCCAAGAGGTCACACAACTGGCTCAAGCTGAAGAAGGACTACCTTGACGGTGTGGGTGACACT
  CTGGACCTTGTGGTGATTGGTGCCTACCTGGGCCGGGGGAAGCGGGCTGGCCGGTATGGAGGCTTCCTGT
  TGGCTGCCTACGATGAGGAGAGTGAAGAGCTTCAGGCTATATGCAAGCTGGGAACTGGATTCAGCGATGA
  GGAGCTGGAGGAACACCACCAGAGCCTGCAGGCCCTGGTATTGCCTACCCCACGCCCCTATGTGAGGATT
  GATGGGGCAGTGGCCCCAGACCATTGGCTGGACCCAAGCATTGTGTGGGAGGTGAAGTGTGCTGATCTTT
  CCCTGTCCCCCATCTACCCTGCTGCACGGGGCCTGGTGGACAAAGAAAAAGGGATCTCTCTTCGTTTTCC
  TCGGTTCATTCGTGTCCGTAAAGACAAGCAGCCAGAGCAGGCCACTACCAGTAACCAGGTTGCCTCTTTG
  TACCGGAAGCAGAGTCAGATTCAGAACCAACAAAGCTCAGACTTGGACTCCGACGTTGAAGACTATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001199310
• Insert Size: 2799 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199310.1, NP_001186239.1
• RefSeq Size: 3194 bp
• RefSeq ORF: 2799 bp
• Locus ID: 16881
• Cytogenetics: 7 7.82 cM
• Gene Summary: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) represents the longest transcript. Variants 1, 2, and 3 encode the same protein.