Brd8 (NM_001289606) Mouse Tagged ORF Clone

CAT#: MR231310

Brd8 (myc-DDK-tagged) - Mouse bromodomain containing 8 (Brd8), transcript variant 2

Product Images

Circular map for MR231310

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Brd8
• Synonyms: 2610007E11Rik; 4432404P07Rik; SMAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231310 representing NM_001289606
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCGGGACAGGCAAGCACAAGCTGCTGAGTACTGGCCCAACAGAGCCATGGTCCATCCGAGAGA
  AGTTGTGTTTAGCATCTTCTGTCATGAGGAGTGGGGATCAGAACTGGGTATCAGTTAGCAGAGCAATCAA
  GCCCTTTGCAGAACCTGGCCGGCCTCCAGACTGGTTCTCTCAAAAACATTGTGCTTCTCAGTATTCTGAG
  CTCTTAGAGACTACTGAGACTCCAAAACGGAAACGGGGTGAAAAAGGAGAAGTGGTAGAAACTGTTGAAG
  ATGTTATTGTTCGCAAACTGACTGCTGAGAGAGTTGAAGAACTGAAGAAAGTCATAAAGGAGACACAGGA
  GAGATACAGGCGTCTGAAAAGAGATGCAGAACTAATCCAAGCCGGGCACATGGACAGCAGACTGGATGAG
  CTCTGCAATGACATTGCAATGAAAAAGAAATTGGAAGAGGAAGAGGCTGAAGTAAAAAGGAAGGCCACCG
  ATGCTGCGTACCAGGCGCGACAAGCAGTGAAAACGCCTCCTCGAAGGTTACCGACTGTGATGGTCCGCTC
  TCCTGTAGACTCTGCCTCCCCAGGAGGTGATTACCCACTTGGAGACTTGACTCCAACCACTATGGAAGAG
  GCTACCTCTGGGGTGAATGAGAGTGAAATGCCTGTACCCCCTGGCCATCTGAACAGCACAGGGGTTCTCT
  TGGAGGTAGGAGGGGTTCTTCCCATGATACATGGTGGGGAGATACAGCCAACAACCAGTGCTGTGGCGGC
  CTCCCCGGCTGCCTCAGGTGCTCCCACTCTTTCCCGGCTTTTAGAAGCTGGGCCCACACAGTTCACCACT
  CCTCTTCCTTCCTTCACTACTGTTGCCAGTGAGCCGCCAGTTAAGCTTGTGCCACCCCCTGTAGAGTCTG
  TGTCCCAGGCTACCATTGTCATGATGCCCGCGCTGCCAGCACCATCCTCTGCTGCGGCTGTCTCCACTTC
  TGAAAGTGGAGCTCCAGTGAGCCAGCCTGAGCCCTGTGTACCCCTGGAAGCTGTGGGGGATCCACATACT
  GTGACTGTTTCCATGGATAGCAATGAAATCTCCATGATCATTAATTCCATCAAAGAAGAGTGTTTCCGCT
  CAGGGGTAGCAGAGGCTCCTGGTGGATCAAAGGCTCCAAGCATAGATGGGAAGGAAGACTTGGATCTAGC
  GGAGAAGATGGATATTGCTGTGTCTTACACAGGTGAAGAGTTGGACTTTGAAACAGTTGGAGACATCATT
  GCCATCATTGAGGACAAGGTGGATGATCACCCTGAAGTGCTGGATGTGGCAGCGGTAGAAGCGGCGTTAT
  CGTTCTGTGAAGAGAATGATGACCCTCAGTCCCTGCCTGGCCCCTGGGAGCACCCTATCCAGCAGGAGCG
  CGACAAGCCAGTACCTCTGCCAGCACCAGAGATGACAGTCAAACAAGAGAGGCTAGACTTCGAGGAATCA
  GAAAACAAAGGGCTCCATGACCTGGTGGACATCAGGGATTCCGGTGTTGAGATTAAGGTGGAACCCACAG
  AGCCAGAGCCAGGCATGTCTGGGGCTGAGATAGTAGCTGGAGTTGGTCCAGTTCCAAGTATGGAGCCACC
  AGAACTCAGGAGTCAAGACTCAGATGAAGAACCTAGAAGTTCTGCAGCTGGAGACATTGGTGAGGCAGAT
  GGTTCCAGTGGGAAAGGCGATGAGAGGCCACTTTCAGCTGTGAAGACAGAGGCATCCCCTGAGAGCATGT
  TGTCTCCATCACATGGCTCAAATCTTATTGAAGATCCTTTAGAGGCAGAGACTCAACACAAGTTTGAAAT
  GTCAGACTCATTGAAAGAAGAATCAGGGACTATTTTTGGAAGCCAGATAAAGGATGCCCCAGGTGACGAT
  GAGGAAGAAGATGGAGTCAGTGAAGCAGCTAGCCTAGAGGAGCCTAAGGAAGAGGATCAAGGAGAAGGCT
  ATTTGTCTGAGATGGATAATGAGCCCCCTGTGAGTGAGAGTGATGATGGCTTTAGTATACATAACGCCAC
  ACTGCAGTCACACACTCTGGCAGACTCCATCCCAAGCAGCCCTGCCTCCTCCCAGTTTTCCGTGTGCAGT
  GAAGATCAAGAAGCAATTCAGGCTCAGAAAATATGGAAGAAAGCCATCATGCTTGTATGGAGGGCTGCAG
  CAAATCATAGGTATGCCAATGTGTTCCTGCAACCTGTTACAGATGACATAGCTCCTGGTTACCATAGCAT
  TGTACAGAGGCCTATGGATTTGTCAACTATAAAGAAAAACATTGAAAATGGACTGATCCGAAGCACAGCT
  GAGTTTCAGCGTGACATCATGCTGATGTTTCAGAATGCTGTTATGTACAATAGCTCAGACCATGATGTCT
  ATCACATGGCAGTAGAGATGCAGAGAGATGTCTTGGAACAGATCCAGCAATTCTTGGCCACACAGTTGAT
  TATGCAAACATCTGAGTCTGGAATCAGTGCTAAAAGTCTCCGGGGGAGAGACTCTACCCGAAAACAAGAT
  GCTTCAGAGAAGGACAGTGTCCCCATGGGCTCTCCTGCCTTCCTTCTCTCTCTCTTTGATGGGGGAACCA
  GGGGACGCCGCTGTGCCATTGAAGCAGATATGAAGATGAAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231310 representing NM_001289606
  Red=Cloning site Green=Tags(s)
  
  MATGTGKHKLLSTGPTEPWSIREKLCLASSVMRSGDQNWVSVSRAIKPFAEPGRPPDWFSQKHCASQYSE
  LLETTETPKRKRGEKGEVVETVEDVIVRKLTAERVEELKKVIKETQERYRRLKRDAELIQAGHMDSRLDE
  LCNDIAMKKKLEEEEAEVKRKATDAAYQARQAVKTPPRRLPTVMVRSPVDSASPGGDYPLGDLTPTTMEE
  ATSGVNESEMPVPPGHLNSTGVLLEVGGVLPMIHGGEIQPTTSAVAASPAASGAPTLSRLLEAGPTQFTT
  PLPSFTTVASEPPVKLVPPPVESVSQATIVMMPALPAPSSAAAVSTSESGAPVSQPEPCVPLEAVGDPHT
  VTVSMDSNEISMIINSIKEECFRSGVAEAPGGSKAPSIDGKEDLDLAEKMDIAVSYTGEELDFETVGDII
  AIIEDKVDDHPEVLDVAAVEAALSFCEENDDPQSLPGPWEHPIQQERDKPVPLPAPEMTVKQERLDFEES
  ENKGLHDLVDIRDSGVEIKVEPTEPEPGMSGAEIVAGVGPVPSMEPPELRSQDSDEEPRSSAAGDIGEAD
  GSSGKGDERPLSAVKTEASPESMLSPSHGSNLIEDPLEAETQHKFEMSDSLKEESGTIFGSQIKDAPGDD
  EEEDGVSEAASLEEPKEEDQGEGYLSEMDNEPPVSESDDGFSIHNATLQSHTLADSIPSSPASSQFSVCS
  EDQEAIQAQKIWKKAIMLVWRAAANHRYANVFLQPVTDDIAPGYHSIVQRPMDLSTIKKNIENGLIRSTA
  EFQRDIMLMFQNAVMYNSSDHDVYHMAVEMQRDVLEQIQQFLATQLIMQTSESGISAKSLRGRDSTRKQD
  ASEKDSVPMGSPAFLLSLFDGGTRGRRCAIEADMKMKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289606
• ORF Size: 2634 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289606.1, NP_001276535.1
• RefSeq Size: 4511 bp
• RefSeq ORF: 2637 bp
• Locus ID: 78656
• UniProt ID: Q8R3B7
• Cytogenetics: 18 B1
• MW: 95.6 kDa
• Gene Summary: May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome.[UniProtKB/Swiss-Prot Function]