Cass4 (NM_001276422) Mouse Tagged ORF Clone

CAT#: MR231134

Cass4 (myc-DDK-tagged) - Mouse Cas scaffolding protein family member 4 (Cass4), transcript variant 3

Product Images

Circular map for MR231134

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cass4
• Synonyms: F730031O20Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231134 representing NM_001276422
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGGCACCAGCATCAGAGAGGGTGCGCCCAAGACCCTGTTGGCCAGGGCACTCTACGACAACCATG
  CTGATTGCTCCGACGAGCTTGCTTTCTCCAGAGGGGACATCCTGACCATCGTGGAACAAAATGTGCCAGA
  AAGCGAGGGCTGGTGGAGGTGTCTGCTCCACGGGAGGCAAGGCCTGGCTCCAGCCAACCGCCTCCAAGTC
  CTCAGAGAGACCCCTGCAGACAGACCCTGTCCCCTGCTCCCGAGAGGCCCAGACACAGATCTCACCAGCT
  CCGGGGCGCCTTACCAGGTGCAGGACTTGATCTCCCCGCCACCCCAGGGACCTGTGTATGAACCAATGAG
  GAGCTGGGTGGAGGGGCCGTCACCAGCGACTGCCCAAGTCTACGAATTGCCTGAGTCACCTTCCAGTGCC
  AGGATCATCTGTGAAAAGACGCTGAGCTTCCCCAAGCAGGCCCTCTCTGTGCTTCCCAGACCGACGAGGG
  CCTCGTTGCCGACACTTCCTTCCCAGGTGTATGACGTGCCTGTCCAGAGGCAGGGCTTCTCAACCCTTGA
  GAGGCTGGAGAAGCAGCAGTTCTATGACATACCCACCAGCTCCCAGAAGGCTCTGCTCCACTCCTCCACC
  AGCCAGGCAAGTGGACGGGATGTTACATTGGCACCGACAATGGCCTTCAGACAAGGTGGTGGCTATAACC
  CGTTATCCAGCCCTCAGAAGTCAGAACGGATTCATGACACTCCGGTGTTGCTGGAAAAGGCCGATGTCAG
  AAATGTGTCTATGACCAGCTTCACCAAAGACTCAGGTTCCAGAGCCATTCCAGGCTCCTCTGCTGTCCAC
  ACTGGAGCTGTGGCCCTCTCCCCACAGCTGGGGAACACTGTGCAGAGAAAAAACAGCCTCCCAGAAGAAC
  CTACCTATGCCTTCCCAACGTCCAGGGACCCACTGCCCTCTGATGCAGGTGGCAGCTACAAGGTCCCTTC
  CAGGTTTCTTATTCCCAGAGTGGAGCAACAGAACACCATGCCTAACATTTATGACACCCCTAAAGCCATG
  CAGGGTGTGTCTCATAACGCCCCTAAAGCCATGCAGGGTGTGTCTCTGGCTGGAAAGGAGCTGGAGAGAG
  GCAGAGAGGCTCCAGAAAATTCCCCCTGGATCTCCGGACAGACAAGTTTCCTGTCTCCTGACTCGGACCG
  ATTATCTGTTGCCAGCTCAGACAGCAGGGCTAGTGTGGTGTCTTCCTGCTCCTCCATATCCATGGACTCC
  TCCTCTGGCTCCTCCTCAGAGGACTCCGTCAAGGAGCTATGGATGGACGTGGACTTTGCAAAGGAGACAG
  CAGTGTCTCTGCAACACAAAGTGGCCAGCTCGGCGGCGGGCCTGCTGCTCTTTGTAAGCAGGACTTGGAG
  GTTCAAAGACTCCTTGGAAACCAACATCCACAGAATCCGCAGGGCCGCTGACCACGTAGAGGAATCTGTA
  AGAGAATTTCTGGATTTTGCCCAAGGGGTTGGTGGGACCGCTTGCAATCTCACAGACAGCTACCTTCAGG
  CTAGAATTAGAGATCAGCTCCAGACAATCTCTAGCTCCTACCAGACACTGCTGGATGCCAAGGGAAGCTT
  GGACCGCTGCAATTGGTCCCTGGAAGTTCTTGTGACAGACAAAGTCCAAAACAGCCTGGATGACCTGGAG
  AGGTTTGTCGCCACTGCACGAATAGTTCCAGAAGACGTCAAGAGGTTTACCTCCATCGTCATTGCCAATG
  GGAAGCTCCTTTTTAAACAGAACTGTGAGAAAGGAGAGATGGACTTGAAGTGTGAGAGATGCATCCGGCC
  TCCCCAAAGAGAAACTGAGTCCTACCAAGAGAGCAGCCCCTTCGACAGGCAACCGACAACTGAACACTCC
  TTTGAACTAGCAAGGAAAAACAGAGTGAATGTCTGTTGGCAGAGTCCTCCCCCTCTTACCTCTCCATCTC
  CCAGTGGGCAAAACACCGAGAGGAAGATCCACCTGTCTAAGCATAGCAGACTCTATTTCGGAGCACTCTT
  CAAAGCCATCAGTGTCTTTGCAAGCAGCCTCAGCAACGGCCAGCCCCCCGAGGTCTTCATCACTCAGAGC
  AAGCTAGTCATCACAGTTGGACAGAAGCTGGTGGACACGCTGTGCAGTGAGACCCAGGAGAAAGATGAAC
  GCAATGAGATCCTGTGTGGCAGCAGTCACCTCTGCGGGCTGCTCAAGGACCTGGCGCTGGCCACCAAAAG
  TGCAGTGATCCAGTACCCGAGCCCCAGTGCCCTGAGCCTCCTCCAGTCAGAGGTGGAGAGGCTAGAGCAC
  CACTCTCGGAAATTCAGAGACACGTTGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231134 representing NM_001276422
  Red=Cloning site Green=Tags(s)
  
  MRGTSIREGAPKTLLARALYDNHADCSDELAFSRGDILTIVEQNVPESEGWWRCLLHGRQGLAPANRLQV
  LRETPADRPCPLLPRGPDTDLTSSGAPYQVQDLISPPPQGPVYEPMRSWVEGPSPATAQVYELPESPSSA
  RIICEKTLSFPKQALSVLPRPTRASLPTLPSQVYDVPVQRQGFSTLERLEKQQFYDIPTSSQKALLHSST
  SQASGRDVTLAPTMAFRQGGGYNPLSSPQKSERIHDTPVLLEKADVRNVSMTSFTKDSGSRAIPGSSAVH
  TGAVALSPQLGNTVQRKNSLPEEPTYAFPTSRDPLPSDAGGSYKVPSRFLIPRVEQQNTMPNIYDTPKAM
  QGVSHNAPKAMQGVSLAGKELERGREAPENSPWISGQTSFLSPDSDRLSVASSDSRASVVSSCSSISMDS
  SSGSSSEDSVKELWMDVDFAKETAVSLQHKVASSAAGLLLFVSRTWRFKDSLETNIHRIRRAADHVEESV
  REFLDFAQGVGGTACNLTDSYLQARIRDQLQTISSSYQTLLDAKGSLDRCNWSLEVLVTDKVQNSLDDLE
  RFVATARIVPEDVKRFTSIVIANGKLLFKQNCEKGEMDLKCERCIRPPQRETESYQESSPFDRQPTTEHS
  FELARKNRVNVCWQSPPPLTSPSPSGQNTERKIHLSKHSRLYFGALFKAISVFASSLSNGQPPEVFITQS
  KLVITVGQKLVDTLCSETQEKDERNEILCGSSHLCGLLKDLALATKSAVIQYPSPSALSLLQSEVERLEH
  HSRKFRDTLE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276422
• ORF Size: 2340 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276422.2
• RefSeq Size: 3633 bp
• RefSeq ORF: 2343 bp
• Locus ID: 320664
• UniProt ID: Q08EC4
• Cytogenetics: 2 H3
• MW: 86.3 kDa
• Gene Summary: Docking protein that plays a role in tyrosine kinase-based signaling related to cell adhesion and cell spreading. Regulates PTK2/FAK1 activity, focal adhesion integrity, and cell spreading (By similarity).[UniProtKB/Swiss-Prot Function]