Cass4 (NM_001276422) Mouse Untagged Clone

CAT#: MC228923

Cass4 (untagged) - Mouse Cas scaffolding protein family member 4 (Cass4), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cass4
• Synonyms: F730031O20Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228923 representing NM_001276422
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGGCACCAGCATCAGAGAGGGTGCGCCCAAGACCCTGTTGGCCAGGGCACTCTACGACAACCATG
  CTGATTGCTCCGACGAGCTTGCTTTCTCCAGAGGGGACATCCTGACCATCGTGGAACAAAATGTGCCAGA
  AAGCGAGGGCTGGTGGAGGTGTCTGCTCCACGGGAGGCAAGGCCTGGCTCCAGCCAACCGCCTCCAAGTC
  CTCAGAGAGACCCCTGCAGACAGACCCTGTCCCCTGCTCCCGAGAGGCCCAGACACAGATCTCACCAGCT
  CCGGGGCGCCTTACCAGGTGCAGGACTTGATCTCCCCGCCACCCCAGGGACCTGTGTATGAACCAATGAG
  GAGCTGGGTGGAGGGGCCGTCACCAGCGACTGCCCAAGTCTACGAATTGCCTGAGTCACCTTCCAGTGCC
  AGGATCATCTGTGAAAAGACGCTGAGCTTCCCCAAGCAGGCCCTCTCTGTGCTTCCCAGACCGACGAGGG
  CCTCGTTGCCGACACTTCCTTCCCAGGTGTATGACGTGCCTGTCCAGAGGCAGGGCTTCTCAACCCTTGA
  GAGGCTGGAGAAGCAGCAGTTCTATGACATACCCACCAGCTCCCAGAAGGCTCTGCTCCACTCCTCCACC
  AGCCAGGCAAGTGGACGGGATGTTACATTGGCACCGACAATGGCCTTCAGACAAGGTGGTGGCTATAACC
  CGTTATCCAGCCCTCAGAAGTCAGAACGGATTCATGACACTCCGGTGTTGCTGGAAAAGGCCGATGTCAG
  AAATGTGTCTATGACCAGCTTCACCAAAGACTCAGGTTCCAGAGCCATTCCAGGCTCCTCTGCTGTCCAC
  ACTGGAGCTGTGGCCCTCTCCCCACAGCTGGGGAACACTGTGCAGAGAAAAAACAGCCTCCCAGAAGAAC
  CTACCTATGCCTTCCCAACGTCCAGGGACCCACTGCCCTCTGATGCAGGTGGCAGCTACAAGGTCCCTTC
  CAGGTTTCTTATTCCCAGAGTGGAGCAACAGAACACCATGCCTAACATTTATGACACCCCTAAAGCCATG
  CAGGGTGTGTCTCATAACGCCCCTAAAGCCATGCAGGGTGTGTCTCTGGCTGGAAAGGAGCTGGAGAGAG
  GCAGAGAGGCTCCAGAAAATTCCCCCTGGATCTCCGGACAGACAAGTTTCCTGTCTCCTGACTCGGACCG
  ATTATCTGTTGCCAGCTCAGACAGCAGGGCTAGTGTGGTGTCTTCCTGCTCCTCCATATCCATGGACTCC
  TCCTCTGGCTCCTCCTCAGAGGACTCCGTCAAGGAGCTATGGATGGACGTGGACTTTGCAAAGGAGACAG
  CAGTGTCTCTGCAACACAAAGTGGCCAGCTCGGCGGCGGGCCTGCTGCTCTTTGTAAGCAGGACTTGGAG
  GTTCAAAGACTCCTTGGAAACCAACATCCACAGAATCCGCAGGGCCGCTGACCACGTAGAGGAATCTGTA
  AGAGAATTTCTGGATTTTGCCCAAGGGGTTGGTGGGACCGCTTGCAATCTCACAGACAGCTACCTTCAGG
  CTAGAATTAGAGATCAGCTCCAGACAATCTCTAGCTCCTACCAGACACTGCTGGATGCCAAGGGAAGCTT
  GGACCGCTGCAATTGGTCCCTGGAAGTTCTTGTGACAGACAAAGTCCAAAACAGCCTGGATGACCTGGAG
  AGGTTTGTCGCCACTGCACGAATAGTTCCAGAAGACGTCAAGAGGTTTACCTCCATCGTCATTGCCAATG
  GGAAGCTCCTTTTTAAACAGAACTGTGAGAAAGGAGAGATGGACTTGAAGTGTGAGAGATGCATCCGGCC
  TCCCCAAAGAGAAACTGAGTCCTACCAAGAGAGCAGCCCCTTCGACAGGCAACCGACAACTGAACACTCC
  TTTGAACTAGCAAGGAAAAACAGAGTGAATGTCTGTTGGCAGAGTCCTCCCCCTCTTACCTCTCCATCTC
  CCAGTGGGCAAAACACCGAGAGGAAGATCCACCTGTCTAAGCATAGCAGACTCTATTTCGGAGCACTCTT
  CAAAGCCATCAGTGTCTTTGCAAGCAGCCTCAGCAACGGCCAGCCCCCCGAGGTCTTCATCACTCAGAGC
  AAGCTAGTCATCACAGTTGGACAGAAGCTGGTGGACACGCTGTGCAGTGAGACCCAGGAGAAAGATGAAC
  GCAATGAGATCCTGTGTGGCAGCAGTCACCTCTGCGGGCTGCTCAAGGACCTGGCGCTGGCCACCAAAAG
  TGCAGTGATCCAGTACCCGAGCCCCAGTGCCCTGAGCCTCCTCCAGTCAGAGGTGGAGAGGCTAGAGCAC
  CACTCTCGGAAATTCAGAGACACGTTGGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276422
• Insert Size: 2343 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276422.1, NP_001263351.1
• RefSeq Size: 3633 bp
• RefSeq ORF: 2343 bp
• Locus ID: 320664
• UniProt ID: Q08EC4
• Cytogenetics: 2 H3
• Gene Summary: Docking protein that plays a role in tyrosine kinase-based signaling related to cell adhesion and cell spreading. Regulates PTK2/FAK1 activity, focal adhesion integrity, and cell spreading (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region and lacks an alternate in-frame exon in the 3' coding region compared to variant 2. It encodes isoform 3, which is shorter than isoform 2.