Eef2k (NM_001267711) Mouse Tagged ORF Clone

CAT#: MR230988

Eef2k (myc-DDK-tagged) - Mouse eukaryotic elongation factor-2 kinase (Eef2k), transcript variant 3

Product Images

Circular map for MR230988

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eef2k
• Synonyms: C86191; eEF-2K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230988 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGACGAAGACCTCATCTTCTGCCTGGAAGGTGTTGACGGTGGCAGGTGCTCCCGAGCTGGCCACA
  ATGCGGACTCTGACACAGACAGTGACGATGATGAGGGCTATTTCATCTGCCCCATCACTGATGACCACAT
  GTCCAATCAGAATGTCAGCTCCAAAGTCCAGAGCTACTATAGCAACCTAACAAAAACAGAGTGCGGCTCC
  ACAGGGTCACCAGCCAGCTCCTTCCACTTCAAGGAAGCCTGGAAGCATGCGATCGAGAAAGCCAAGCACA
  TGCCTGACCCCTGGGCTGAATTCCATCTCGAGGACATCGCCACAGAACATGCTACTCGGCACAGGTACAA
  CGCTGTCACCGGGGAATGGCTGAAAGACGAGGTTCTGATCAAGATGGCGTCTCAGCCCTTCGGCCGTGGA
  GCAATGAGGGAGTGCTTCAGGACGAAGAAACTCTCCAACTTCTTGCACGCCCAGCAATGGAAGGGGGCCT
  CCAACTACGTGGCCAAGCGCTACATCGAGCCGGTGGACAGGAGCGTGTACTTTGAGGATGTGCAGCTCCA
  GATGGAGGCGAAGCTCTGGGGGGAGGATTACAATCGGCACAAGCCCCCCAAGCAGGTGGATATCATGCAG
  ATGTGCATCATTGAGCTAAAGGACAGACCAGGCCAGCCCCTCTTCCACTTGGAGCACTACATTGAGGGCA
  AGTACATCAAGTACAATTCCAACTCAGGCTTTGTCCGTGATGACAACATCCGACTAACCCCACAGGCCTT
  CAGCCATTTCACATTTGAGCGTTCTGGTCATCAGCTGATTGTAGTGGACATCCAGGGTGTGGGTGACCTT
  TATACCGACCCACAGATCCACACTGAGAAAGGCACTGACTTTGGAGATGGTAACCTTGGTGTCCGGGGAA
  TGGCTCTCTTCTTCTACTCTCATGCCTGCAACCGGATTTGTCAGAGCATGGGCCTTACGCCCTTTGACCT
  CTCCCCACGGGAACAGGATGCGGTGAATCAGAGCACCAGGCTATTGCAATCAGCCAAGACCATCTTGAGG
  GGGACAGAGGAGAAGTGTGGGAGTCCCCGCATAAGGACACTCTCTAGCAGCCGGCCCCCTTTGCTCCTTC
  GCCTGTCAGAGAACTCCGGGGATGAGAACATGAGTGACGTGACCTTTGACTCTCTGCCTTCCTCCCCGTC
  TTCAGCTACACCACACAGCCAGAAACTGGACCACCTCCATTGGCCAGTGTTTGGTGACCTCGATAACATG
  GGCCCTAGAGACCATGACCGCATGGACAATCACCGGGACTCTGAGAATAGTGGGGACAGTGGGTATCCAA
  GCGAGAAGCGAAGTGACCTGGATGATCCTGAGCCCCGAGAACACGGCCACTCCAACGGCAACCGAAGGCA
  TGAATCTGACGAGGATAGCCTGGGCAGCTCTGGACGGGTCTGTGTGGAGACGTGGAACCTGCTCAATCCC
  TCCCGCCTGCACCTGCCGAGGCCCTCGGCCGTGGCCCTAGAAGTGCAGAGGCTAAATGCCCTGGACCTTG
  GAAGGAAAATCGGGAAGTCTGTTTTGGGGAAGGTCCATTTGGCCATGGTGCGATACCACGAGGGCGGGCG
  CTTCTGCGAGAAGGATGAGGAGTGGGATCGAGAGTCAGCCATCTTCCATCTGGAGCATGCAGCTGACCTG
  GGAGAACTGGAGGCCATCGTGGGCCTAGGCCTCATGTACTCTCAGCTGCCCCACCACATCCTGGCTGATG
  TCTCTCTGAAGGAGACAGAGGAGAACAAGACAAAAGGCTTTGATTACTTACTGAAGGCGGCAGAAGCTGG
  TGACAGGCATTCCATGATTTTAGTGGCCCGAGCTTTTGACACTGGCCTGAACCTCAGCCCAGACAGGTGT
  CAAGACTGGTCGGAAGCCTTGCACTGGTACAACACAGCCCTGGAGACAACAGACTGCGATGAAGGCGGGG
  AGTACGATGGGATACAGGACGAGCCCCAGTACGCACTGCTGGCCAGGGAGGCGGAGATGCTGCTCACCGG
  GGGATTTGGACTGGACAAGAACCCCCAAAGATCAGGAGATTTGTACACCCAGGCAGCTGAGGCAGCAATG
  GAAGCCATGAAGGGCCGGCTAGCCAACCAGTACTACGAGAAGGCGGAAGAGGCCTGGGCCCAGATGGAGG
  AA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230988 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADEDLIFCLEGVDGGRCSRAGHNADSDTDSDDDEGYFICPITDDHMSNQNVSSKVQSYYSNLTKTECGS
  TGSPASSFHFKEAWKHAIEKAKHMPDPWAEFHLEDIATEHATRHRYNAVTGEWLKDEVLIKMASQPFGRG
  AMRECFRTKKLSNFLHAQQWKGASNYVAKRYIEPVDRSVYFEDVQLQMEAKLWGEDYNRHKPPKQVDIMQ
  MCIIELKDRPGQPLFHLEHYIEGKYIKYNSNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDL
  YTDPQIHTEKGTDFGDGNLGVRGMALFFYSHACNRICQSMGLTPFDLSPREQDAVNQSTRLLQSAKTILR
  GTEEKCGSPRIRTLSSSRPPLLLRLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFGDLDNM
  GPRDHDRMDNHRDSENSGDSGYPSEKRSDLDDPEPREHGHSNGNRRHESDEDSLGSSGRVCVETWNLLNP
  SRLHLPRPSAVALEVQRLNALDLGRKIGKSVLGKVHLAMVRYHEGGRFCEKDEEWDRESAIFHLEHAADL
  GELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDYLLKAAEAGDRHSMILVARAFDTGLNLSPDRC
  QDWSEALHWYNTALETTDCDEGGEYDGIQDEPQYALLAREAEMLLTGGFGLDKNPQRSGDLYTQAAEAAM
  EAMKGRLANQYYEKAEEAWAQMEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001267711
• ORF Size: 2172 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001267711.1, NP_001254640.1
• RefSeq Size: 6277 bp
• RefSeq ORF: 2175 bp
• Locus ID: 13631
• UniProt ID: O08796
• Cytogenetics: 7 F2
• MW: 81.7 kDa
• Gene Summary: Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.[UniProtKB/Swiss-Prot Function]