Eef2k (NM_001267711) Mouse Untagged Clone

CAT#: MC228777

Eef2k (untagged) - Mouse eukaryotic elongation factor-2 kinase (Eef2k), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eef2k
• Synonyms: C86191; eEF-2K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228777 representing NM_001267711
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGACGAAGACCTCATCTTCTGCCTGGAAGGTGTTGACGGTGGCAGGTGCTCCCGAGCTGGCCACA
  ATGCGGACTCTGACACAGACAGTGACGATGATGAGGGCTATTTCATCTGCCCCATCACTGATGACCACAT
  GTCCAATCAGAATGTCAGCTCCAAAGTCCAGAGCTACTATAGCAACCTAACAAAAACAGAGTGCGGCTCC
  ACAGGGTCACCAGCCAGCTCCTTCCACTTCAAGGAAGCCTGGAAGCATGCGATCGAGAAAGCCAAGCACA
  TGCCTGACCCCTGGGCTGAATTCCATCTCGAGGACATCGCCACAGAACATGCTACTCGGCACAGGTACAA
  CGCTGTCACCGGGGAATGGCTGAAAGACGAGGTTCTGATCAAGATGGCGTCTCAGCCCTTCGGCCGTGGA
  GCAATGAGGGAGTGCTTCAGGACGAAGAAACTCTCCAACTTCTTGCACGCCCAGCAATGGAAGGGGGCCT
  CCAACTACGTGGCCAAGCGCTACATCGAGCCGGTGGACAGGAGCGTGTACTTTGAGGATGTGCAGCTCCA
  GATGGAGGCGAAGCTCTGGGGGGAGGATTACAATCGGCACAAGCCCCCCAAGCAGGTGGATATCATGCAG
  ATGTGCATCATTGAGCTAAAGGACAGACCAGGCCAGCCCCTCTTCCACTTGGAGCACTACATTGAGGGCA
  AGTACATCAAGTACAATTCCAACTCAGGCTTTGTCCGTGATGACAACATCCGACTAACCCCACAGGCCTT
  CAGCCATTTCACATTTGAGCGTTCTGGTCATCAGCTGATTGTAGTGGACATCCAGGGTGTGGGTGACCTT
  TATACCGACCCACAGATCCACACTGAGAAAGGCACTGACTTTGGAGATGGTAACCTTGGTGTCCGGGGAA
  TGGCTCTCTTCTTCTACTCTCATGCCTGCAACCGGATTTGTCAGAGCATGGGCCTTACGCCCTTTGACCT
  CTCCCCACGGGAACAGGATGCGGTGAATCAGAGCACCAGGCTATTGCAATCAGCCAAGACCATCTTGAGG
  GGGACAGAGGAGAAGTGTGGGAGTCCCCGCATAAGGACACTCTCTAGCAGCCGGCCCCCTTTGCTCCTTC
  GCCTGTCAGAGAACTCCGGGGATGAGAACATGAGTGACGTGACCTTTGACTCTCTGCCTTCCTCCCCGTC
  TTCAGCTACACCACACAGCCAGAAACTGGACCACCTCCATTGGCCAGTGTTTGGTGACCTCGATAACATG
  GGCCCTAGAGACCATGACCGCATGGACAATCACCGGGACTCTGAGAATAGTGGGGACAGTGGGTATCCAA
  GCGAGAAGCGAAGTGACCTGGATGATCCTGAGCCCCGAGAACACGGCCACTCCAACGGCAACCGAAGGCA
  TGAATCTGACGAGGATAGCCTGGGCAGCTCTGGACGGGTCTGTGTGGAGACGTGGAACCTGCTCAATCCC
  TCCCGCCTGCACCTGCCGAGGCCCTCGGCCGTGGCCCTAGAAGTGCAGAGGCTAAATGCCCTGGACCTTG
  GAAGGAAAATCGGGAAGTCTGTTTTGGGGAAGGTCCATTTGGCCATGGTGCGATACCACGAGGGCGGGCG
  CTTCTGCGAGAAGGATGAGGAGTGGGATCGAGAGTCAGCCATCTTCCATCTGGAGCATGCAGCTGACCTG
  GGAGAACTGGAGGCCATCGTGGGCCTAGGCCTCATGTACTCTCAGCTGCCCCACCACATCCTGGCTGATG
  TCTCTCTGAAGGAGACAGAGGAGAACAAGACAAAAGGCTTTGATTACTTACTGAAGGCGGCAGAAGCTGG
  TGACAGGCATTCCATGATTTTAGTGGCCCGAGCTTTTGACACTGGCCTGAACCTCAGCCCAGACAGGTGT
  CAAGACTGGTCGGAAGCCTTGCACTGGTACAACACAGCCCTGGAGACAACAGACTGCGATGAAGGCGGGG
  AGTACGATGGGATACAGGACGAGCCCCAGTACGCACTGCTGGCCAGGGAGGCGGAGATGCTGCTCACCGG
  GGGATTTGGACTGGACAAGAACCCCCAAAGATCAGGAGATTTGTACACCCAGGCAGCTGAGGCAGCAATG
  GAAGCCATGAAGGGCCGGCTAGCCAACCAGTACTACGAGAAGGCGGAAGAGGCCTGGGCCCAGATGGAGG
  AATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001267711
• Insert Size: 2175 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001267711.1, NP_001254640.1
• RefSeq Size: 6277 bp
• RefSeq ORF: 2175 bp
• Locus ID: 13631
• UniProt ID: O08796
• Cytogenetics: 7 F2
• Gene Summary: Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.