Atp2c1 (NM_001253836) Mouse Tagged ORF Clone

CAT#: MR230836

Atp2c1 (myc-DDK-tagged) - Mouse ATPase, Ca++-sequestering (Atp2c1), transcript variant 4

Product Images

Circular map for MR230836

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Atp2c1
• Synonyms: 1700121J11Rik; ATP2C1A; AW061228; BCPM; D930003G21Rik; HHD; pmr1; SPCA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230836 representing NM_001253836
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCCTGTACTGACGTCAAAGAGAGCAAGTGAGTTAGCAGTCAGTGAGGTTGCAGGCCTTCTCCAGG
  CTGATCTTCAGAATGGCTTAAACAAATCTGAAGTTAGTCATAGGCGAGCCTTCCATGGTTGGAATGAATT
  TGATATCAGTGAAGATGAACCATTATGGAAGAAGTATATTTCTCAGTTTAAAAATCCCCTTATCATGCTG
  CTCCTGGCGTCTGCGGTCATCAGCATTTTAATGCGCCAGTTTGATGATGCCGTCAGTATCACTGTGGCAA
  TAGTAATTGTCGTCACTGTGGCCTTTGTTCAGGAATATCGTTCAGAAAAATCTCTAGAAGAATTGAGTAA
  ACTTGTGCCACCAGAATGCCATTGTGTGCGTGAAGGAAAACTGGAGCATACACTTGCCCGAGACTTGGTT
  CCAGGTGACACAGTTTGTCTCTCTGTGGGGGACAGAGTTCCTGCGGACTTACGCTTATTTGAGGCTGTGG
  ATCTTTCTGTGGATGAGTCTAGCTTGACAGGAGAGACAGCTCCGTGCTCTAAAGTGACAGCCCCTCAGCC
  AGCTGCTAATGGAGATCTTGCGTCAAGAAGTAACATCGCCTTCATGGGGACACTGGTCAGATGTGGCAAA
  GCAAAGGGTATTGTCATTGGAACAGGAGAAAATTCTGAATTTGGAGAGGTCTTTAAGATGATGCAGGCAG
  AAGAAGCACCAAAAACTCCTCTGCAGAAGAGCATGGACCTCTTAGGCAAGCAGCTGTCCTTTTACTCCTT
  TGGTATAATAGGTATCATCATGTTGGTTGGCTGGTTACTAGGAAAAGACATTCTGGAAATGTTCACTATT
  AGTGTAAGCTTGGCTGTAGCTGCAATTCCTGAAGGCCTGCCTATTGTGGTCACGGTGACTCTAGCCCTTG
  GTGTTATGAGAATGGTGAAGAAAAGGGCTATTGTAAAGAAATTGCCTATTGTGGAAACACTGGGCTGCTG
  TAATGTGATTTGTTCAGATAAAACTGGAACCCTGACGAAGAACGAGATGACTGTTACTCACATCCTCACT
  TCCGATGGCCTGCATGCTGAGGTCACTGGAGTTGGCTATAATCAGTTTGGTGAAGTGATCGTTGATGGTG
  ATGTTGTTCATGGATTTTATAACCCAGCTGTTAGCAGAATTGTTGAGGCAGGCTGTGTGTGCAATGATGC
  TGTAATTAGGAACAACACTCTGATGGGAAAGCCAACTGAAGGAGCCTTAATCGCTCTTGCTATGAAGATG
  GGTCTTGATGGACTGCAACAAGACTACATCAGGAAAGCTGAATACCCTTTTAGTTCTGAGCAGAAGTGGA
  TGGCTGTTAAGTGTGTGCACCGAACACAGCAGGACAGACCAGAGATTTGTTTTATGAAGGGTGCTTATGA
  GCAGGTGATTAAGTATTGTACTACATACAACAGCAAAGGGCAGACTTTGGCACTTACCCAGCAGCAGAGA
  GATTTGTATCAACAAGAGAAGGCACGGATGGGCTCAGCGGGACTCAGAGTTCTTGCCTTGGCGTCTGGTC
  CGGAACTGGGGCAGCTGACCTTCCTTGGCCTGGTCGGAATCATTGACCCTCCTAGAACTGGTGTGAAGGA
  AGCTGTCACAACACTCATTGCCTCAGGAGTCTCCATCAAAATGATCACTGGAGATTCTCAGGAGACTGCA
  ATTGCCATCGCTAGTCGTCTGGGATTGTACTCTAAGACTTCACAGTCCGTGTCTGGGGAAGAAGTCGATA
  CAATGGAGGTGCAGCACCTTTCACAGATAGTGCCAAAGGTTGCAGTATTTTACAGAGCAAGCCCAAGACA
  CAAGATGAAAATTATTAAGTCTCTACAGAAGAACGGGGCAGTTGTAGCCATGACAGGAGATGGGGTAAAT
  GATGCAGTTGCTCTCAAGGCTGCAGACATTGGAGTTGCGATGGGCCAGACTGGCACCGATGTTTGCAAAG
  AGGCTGCAGACATGATCTTGGTGGATGATGATTTCCAAACCATCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230836 representing NM_001253836
  Red=Cloning site Green=Tags(s)
  
  MIPVLTSKRASELAVSEVAGLLQADLQNGLNKSEVSHRRAFHGWNEFDISEDEPLWKKYISQFKNPLIML
  LLASAVISILMRQFDDAVSITVAIVIVVTVAFVQEYRSEKSLEELSKLVPPECHCVREGKLEHTLARDLV
  PGDTVCLSVGDRVPADLRLFEAVDLSVDESSLTGETAPCSKVTAPQPAANGDLASRSNIAFMGTLVRCGK
  AKGIVIGTGENSEFGEVFKMMQAEEAPKTPLQKSMDLLGKQLSFYSFGIIGIIMLVGWLLGKDILEMFTI
  SVSLAVAAIPEGLPIVVTVTLALGVMRMVKKRAIVKKLPIVETLGCCNVICSDKTGTLTKNEMTVTHILT
  SDGLHAEVTGVGYNQFGEVIVDGDVVHGFYNPAVSRIVEAGCVCNDAVIRNNTLMGKPTEGALIALAMKM
  GLDGLQQDYIRKAEYPFSSEQKWMAVKCVHRTQQDRPEICFMKGAYEQVIKYCTTYNSKGQTLALTQQQR
  DLYQQEKARMGSAGLRVLALASGPELGQLTFLGLVGIIDPPRTGVKEAVTTLIASGVSIKMITGDSQETA
  IAIASRLGLYSKTSQSVSGEEVDTMEVQHLSQIVPKVAVFYRASPRHKMKIIKSLQKNGAVVAMTGDGVN
  DAVALKAADIGVAMGQTGTDVCKEAADMILVDDDFQTIM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001253836
• ORF Size: 2007 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253836.1, NP_001240765.1
• RefSeq Size: 2482 bp
• RefSeq ORF: 2010 bp
• Locus ID: 235574
• Cytogenetics: 9 F1
• MW: 72.8 kDa
• Gene Summary: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium.[UniProtKB/Swiss-Prot Function]