Atp2c1 (NM_001253836) Mouse Untagged Clone

CAT#: MC228625

Atp2c1 (untagged) - Mouse ATPase, Ca++-sequestering (Atp2c1), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atp2c1
• Synonyms: 1700121J11Rik; ATP2C1A; AW061228; BCPM; D930003G21Rik; HHD; pmr1; SPCA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228625 representing NM_001253836
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCCTGTACTGACGTCAAAGAGAGCAAGTGAGTTAGCAGTCAGTGAGGTTGCAGGCCTTCTCCAGG
  CTGATCTTCAGAATGGCTTAAACAAATCTGAAGTTAGTCATAGGCGAGCCTTCCATGGTTGGAATGAATT
  TGATATCAGTGAAGATGAACCATTATGGAAGAAGTATATTTCTCAGTTTAAAAATCCCCTTATCATGCTG
  CTCCTGGCGTCTGCGGTCATCAGCATTTTAATGCGCCAGTTTGATGATGCCGTCAGTATCACTGTGGCAA
  TAGTAATTGTCGTCACTGTGGCCTTTGTTCAGGAATATCGTTCAGAAAAATCTCTAGAAGAATTGAGTAA
  ACTTGTGCCACCAGAATGCCATTGTGTGCGTGAAGGAAAACTGGAGCATACACTTGCCCGAGACTTGGTT
  CCAGGTGACACAGTTTGTCTCTCTGTGGGGGACAGAGTTCCTGCGGACTTACGCTTATTTGAGGCTGTGG
  ATCTTTCTGTGGATGAGTCTAGCTTGACAGGAGAGACAGCTCCGTGCTCTAAAGTGACAGCCCCTCAGCC
  AGCTGCTAATGGAGATCTTGCGTCAAGAAGTAACATCGCCTTCATGGGGACACTGGTCAGATGTGGCAAA
  GCAAAGGGTATTGTCATTGGAACAGGAGAAAATTCTGAATTTGGAGAGGTCTTTAAGATGATGCAGGCAG
  AAGAAGCACCAAAAACTCCTCTGCAGAAGAGCATGGACCTCTTAGGCAAGCAGCTGTCCTTTTACTCCTT
  TGGTATAATAGGTATCATCATGTTGGTTGGCTGGTTACTAGGAAAAGACATTCTGGAAATGTTCACTATT
  AGTGTAAGCTTGGCTGTAGCTGCAATTCCTGAAGGCCTGCCTATTGTGGTCACGGTGACTCTAGCCCTTG
  GTGTTATGAGAATGGTGAAGAAAAGGGCTATTGTAAAGAAATTGCCTATTGTGGAAACACTGGGCTGCTG
  TAATGTGATTTGTTCAGATAAAACTGGAACCCTGACGAAGAACGAGATGACTGTTACTCACATCCTCACT
  TCCGATGGCCTGCATGCTGAGGTCACTGGAGTTGGCTATAATCAGTTTGGTGAAGTGATCGTTGATGGTG
  ATGTTGTTCATGGATTTTATAACCCAGCTGTTAGCAGAATTGTTGAGGCAGGCTGTGTGTGCAATGATGC
  TGTAATTAGGAACAACACTCTGATGGGAAAGCCAACTGAAGGAGCCTTAATCGCTCTTGCTATGAAGATG
  GGTCTTGATGGACTGCAACAAGACTACATCAGGAAAGCTGAATACCCTTTTAGTTCTGAGCAGAAGTGGA
  TGGCTGTTAAGTGTGTGCACCGAACACAGCAGGACAGACCAGAGATTTGTTTTATGAAGGGTGCTTATGA
  GCAGGTGATTAAGTATTGTACTACATACAACAGCAAAGGGCAGACTTTGGCACTTACCCAGCAGCAGAGA
  GATTTGTATCAACAAGAGAAGGCACGGATGGGCTCAGCGGGACTCAGAGTTCTTGCCTTGGCGTCTGGTC
  CGGAACTGGGGCAGCTGACCTTCCTTGGCCTGGTCGGAATCATTGACCCTCCTAGAACTGGTGTGAAGGA
  AGCTGTCACAACACTCATTGCCTCAGGAGTCTCCATCAAAATGATCACTGGAGATTCTCAGGAGACTGCA
  ATTGCCATCGCTAGTCGTCTGGGATTGTACTCTAAGACTTCACAGTCCGTGTCTGGGGAAGAAGTCGATA
  CAATGGAGGTGCAGCACCTTTCACAGATAGTGCCAAAGGTTGCAGTATTTTACAGAGCAAGCCCAAGACA
  CAAGATGAAAATTATTAAGTCTCTACAGAAGAACGGGGCAGTTGTAGCCATGACAGGAGATGGGGTAAAT
  GATGCAGTTGCTCTCAAGGCTGCAGACATTGGAGTTGCGATGGGCCAGACTGGCACCGATGTTTGCAAAG
  AGGCTGCAGACATGATCTTGGTGGATGATGATTTCCAAACCATCATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001253836
• Insert Size: 2010 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253836.1, NP_001240765.1
• RefSeq Size: 2482 bp
• RefSeq ORF: 2010 bp
• Locus ID: 235574
• Cytogenetics: 9 F1
• Gene Summary: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) has a multiple differences, compared to variant 1. These differences result in distinct 5' and 3' UTRs and cause translation initiation at a downstream start codon, compared to variant 1. The encoded protein (isoform 3) is shorter than isoform 1.