C8a (NM_001290645) Mouse Tagged ORF Clone

CAT#: MR230452

C8a (myc-DDK-tagged) - Mouse complement component 8, alpha polypeptide (C8a), transcript variant 2

Product Images

Circular map for MR230452

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C8a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230452 representing NM_001290645
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCCTGGGGTAACTGCACAAGAGAAGGTGAACCATACCGATACCGGAGCCTCTTGCAGCCAAGCA
  AGTTTGGAGGAACCATCTGCAGTGGTGATATCTGGGATGAGGCCAGCTGTGACAGCCCCACACCTTGTCT
  AAGGCAAGCACAGTGTGGACAGGATTTCCAGTGTAGAGAGACAGGTCGCTGCCTGAAACGCCACCTGGTG
  TGTAACGGAGATAACGACTGCCTGGATGGCTCTGATGAGTCCGACTGTGAAGATGTCAGGGTCACTGAGG
  ATGATTGCCATCAGTATGAACCAATCCCAGGATCAGAAAGGGCAGCCTTGGGGTACAACATCCTGACTCA
  AGAGGAAGCTCAGAGTGTTTACGATGCTAAGTATTATGGGGGCCAGTGTGAAACTGTGTACAATGGAGAC
  TGGAGAAAGCTGCGGTACGACCCCACCTGTGAGCGCCTCTACTATGGGGAGGATGAGAAATACTTTCGGA
  AGCCCTACAACTTCCTTAAGTACCACTTTGAAGCCCTGGCGGATACTTCAATCTCCTCAGAGTTTTATGA
  TGATGCGAATGATCTTTTTTTTCACATAAAGAATGGCAAGTCCCACTCAGCTGGTGTAACCGTTGGCGTA
  GCCCCAGTAAAAAGTCCTGTCTCAATAGAGGTGACTGGATCCGGGTCCAAAGCATCTTCATTCCTGAACA
  AGTTAAACAAGTATAATGAGAAGAGATATGGCTTCATGAGAGTGTCTACAAAGATACAGACTGCACAGTT
  TAAGATGAGAAGGAACAATATTGTGCTGGATGAAGGAATGCTGCAGTCATTAATGGAGCTCCCAGAGCAG
  TTCAACTATGGCATGTACGCCAAGTTCATCAATGACTATGGCACCCACTACATCACCTCTGGAACCATGG
  GTGGCATTTATGAATATGTTATGGTGCTTGACAAAGAGAAAATGAAAACTGAAGGTACTACTGTGGATGA
  AGTCCAAAAATGCATTGGCGGTGGCATTGGCATAGGAATTAAGGATAGTACAATTGAAGGAGTAGGTATA
  TCAGGAGAGTTTTGTGAAAACTCTGGAGATGGTGACCGTGACATAAGGAAGAAAATCACAGGAGTGGAGG
  ACATCATTTCTCGGGTGCAAGGTGGAAGTTCTGTCTGGGGCAGTGTCTTGACACACAACAGCAGTGCCAT
  TACATATCAGTCCTGGGGGAGATCGTTAAAGTATAACCCTGTTGTTATCGATTTTGAGATGCAGCCTATC
  TACCAGCTACTGCGGCACACAAACTTGGGACCCCTGGAGACGAAACGTCAGAACCTGCGACGGGCTTTGG
  ACCAGTACCTGATGGAATTTAATGCCTGTCGTTGTGGGCCCTGCTTCAACAATGGGGAACCCATACTTGA
  CGGCACCAACTGCCGGTGTCAGTGTAGTATGGGCCGGCAGGGCCTGGCCTGTGAACGCACGGTGATAGAG
  GGGCTCAAAGACTTCAAAGCCGCTGGCCACTGGAGCTGCTGGAGTTCCTGGTCAGAGTGCAGAGGAGGTT
  CCCAGGAGAGGAGGAGACAGTGCAACAACCCACCACCCAAGAATGGAGGTACCCCATGCCTAGGGAGGAA
  CCTGCAGACCCAGGCCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230452 representing NM_001290645
  Red=Cloning site Green=Tags(s)
  
  MAPWGNCTREGEPYRYRSLLQPSKFGGTICSGDIWDEASCDSPTPCLRQAQCGQDFQCRETGRCLKRHLV
  CNGDNDCLDGSDESDCEDVRVTEDDCHQYEPIPGSERAALGYNILTQEEAQSVYDAKYYGGQCETVYNGD
  WRKLRYDPTCERLYYGEDEKYFRKPYNFLKYHFEALADTSISSEFYDDANDLFFHIKNGKSHSAGVTVGV
  APVKSPVSIEVTGSGSKASSFLNKLNKYNEKRYGFMRVSTKIQTAQFKMRRNNIVLDEGMLQSLMELPEQ
  FNYGMYAKFINDYGTHYITSGTMGGIYEYVMVLDKEKMKTEGTTVDEVQKCIGGGIGIGIKDSTIEGVGI
  SGEFCENSGDGDRDIRKKITGVEDIISRVQGGSSVWGSVLTHNSSAITYQSWGRSLKYNPVVIDFEMQPI
  YQLLRHTNLGPLETKRQNLRRALDQYLMEFNACRCGPCFNNGEPILDGTNCRCQCSMGRQGLACERTVIE
  GLKDFKAAGHWSCWSSWSECRGGSQERRRQCNNPPPKNGGTPCLGRNLQTQAC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290645
• ORF Size: 1629 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290645.2, NP_001277574.1
• RefSeq Size: 3532 bp
• RefSeq ORF: 1632 bp
• Locus ID: 230558
• Cytogenetics: 4 C6
• MW: 61.5 kDa
• Gene Summary: This gene encodes the alpha subunit of complement component C8 that participates in the assembly of the complement membrane attack complex. The encoded preproprotein undergoes proteolytic processing to generate the alpha subunit, which associates with the beta and gamma subunits to form a trimeric complement component, C8. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. This gene is located adjacent to the gene encoding the beta subunit. [provided by RefSeq, Oct 2015]